Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Dec;174(23):7770–7777. doi: 10.1128/jb.174.23.7770-7777.1992

Derivation of a physical map of the chromosome of Bordetella pertussis Tohama I.

S Stibitz 1, T L Garletts 1
PMCID: PMC207492  PMID: 1447143

Abstract

We have used pulsed-field gel electrophoresis to derive a restriction map of the chromosome of Bordetella pertussis for the enzymes XbaI, SpeI, PacI, and PmeI, which cleave 25, 16, 2, and 1 times, respectively. The apparent size of the genome is 3,750 kb. The positions of genes for major virulence determinants in the vir regulon and of some housekeeping genes were determined. Apart from the previously known linkage of the vir and fha loci, no significant linkage of virulence genes was demonstrated.

Full text

PDF
7770

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aricò B., Rappuoli R. Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes. J Bacteriol. 1987 Jun;169(6):2847–2853. doi: 10.1128/jb.169.6.2847-2853.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aricò B., Scarlato V., Monack D. M., Falkow S., Rappuoli R. Structural and genetic analysis of the bvg locus in Bordetella species. Mol Microbiol. 1991 Oct;5(10):2481–2491. doi: 10.1111/j.1365-2958.1991.tb02093.x. [DOI] [PubMed] [Google Scholar]
  3. Aricó B., Miller J. F., Roy C., Stibitz S., Monack D., Falkow S., Gross R., Rappuoli R. Sequences required for expression of Bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6671–6675. doi: 10.1073/pnas.86.17.6671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beattie D. T., Shahin R., Mekalanos J. J. A vir-repressed gene of Bordetella pertussis is required for virulence. Infect Immun. 1992 Feb;60(2):571–577. doi: 10.1128/iai.60.2.571-577.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Black W. J., Falkow S. Construction and characterization of Bordetella pertussis toxin mutants. Infect Immun. 1987 Oct;55(10):2465–2470. doi: 10.1128/iai.55.10.2465-2470.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Charles I. G., Dougan G., Pickard D., Chatfield S., Smith M., Novotny P., Morrissey P., Fairweather N. F. Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis. Proc Natl Acad Sci U S A. 1989 May;86(10):3554–3558. doi: 10.1073/pnas.86.10.3554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Domenighini M., Relman D., Capiau C., Falkow S., Prugnola A., Scarlato V., Rappuoli R. Genetic characterization of Bordetella pertussis filamentous haemagglutinin: a protein processed from an unusually large precursor. Mol Microbiol. 1990 May;4(5):787–800. doi: 10.1111/j.1365-2958.1990.tb00649.x. [DOI] [PubMed] [Google Scholar]
  8. Endoh M., Amitani M., Nakase Y. Purification and characterization of heat-labile toxin from Bordetella bronchiseptica. Microbiol Immunol. 1986;30(7):659–673. doi: 10.1111/j.1348-0421.1986.tb02992.x. [DOI] [PubMed] [Google Scholar]
  9. Endoh M., Nagai M., Ueda T., Yoshida Y., Nakase Y. Cytopathic effect of heat-labile toxin of Bordetella parapertussis on aortic smooth muscle cells from pigs or guinea pigs. Microbiol Immunol. 1988;32(4):423–428. doi: 10.1111/j.1348-0421.1988.tb01401.x. [DOI] [PubMed] [Google Scholar]
  10. Favre D., Viret J. F. Nucleotide sequence of the recA gene of Bordetella pertussis. Nucleic Acids Res. 1990 Jul 25;18(14):4243–4243. doi: 10.1093/nar/18.14.4243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gentry-Weeks C. R., Cookson B. T., Goldman W. E., Rimler R. B., Porter S. B., Curtiss R., 3rd Dermonecrotic toxin and tracheal cytotoxin, putative virulence factors of Bordetella avium. Infect Immun. 1988 Jul;56(7):1698–1707. doi: 10.1128/iai.56.7.1698-1707.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glaser P., Ladant D., Sezer O., Pichot F., Ullmann A., Danchin A. The calmodulin-sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia coli. Mol Microbiol. 1988 Jan;2(1):19–30. [PubMed] [Google Scholar]
  13. KASUGA T., NAKASE Y., UKISHIMA K., TAKATSU K. Studies on Haemophilus pertussis. V. Relation between the phase of bacilli and the progress of the whooping-cough. Kitasato Arch Exp Med. 1954 Sep;27(3):57–62. [PubMed] [Google Scholar]
  14. Knapp S., Mekalanos J. J. Two trans-acting regulatory genes (vir and mod) control antigenic modulation in Bordetella pertussis. J Bacteriol. 1988 Nov;170(11):5059–5066. doi: 10.1128/jb.170.11.5059-5066.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kobisch M., Novotny P. Identification of a 68-kilodalton outer membrane protein as the major protective antigen of Bordetella bronchiseptica by using specific-pathogen-free piglets. Infect Immun. 1990 Feb;58(2):352–357. doi: 10.1128/iai.58.2.352-357.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  17. Li L. J., Dougan G., Novotny P., Charles I. G. P.70 pertactin, an outer-membrane protein from Bordetella parapertussis: cloning, nucleotide sequence and surface expression in Escherichia coli. Mol Microbiol. 1991 Feb;5(2):409–417. doi: 10.1111/j.1365-2958.1991.tb02123.x. [DOI] [PubMed] [Google Scholar]
  18. Li Z. M., Hannah J. H., Stibitz S., Nguyen N. Y., Manclark C. R., Brennan M. J. Cloning and sequencing of the structural gene for the porin protein of Bordetella pertussis. Mol Microbiol. 1991 Jul;5(7):1649–1656. doi: 10.1111/j.1365-2958.1991.tb01912.x. [DOI] [PubMed] [Google Scholar]
  19. Livey I., Duggleby C. J., Robinson A. Cloning and nucleotide sequence analysis of the serotype 2 fimbrial subunit gene of Bordetella pertussis. Mol Microbiol. 1987 Sep;1(2):203–209. doi: 10.1111/j.1365-2958.1987.tb00513.x. [DOI] [PubMed] [Google Scholar]
  20. Locht C., Geoffroy M. C., Renauld G. Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families. EMBO J. 1992 Sep;11(9):3175–3183. doi: 10.1002/j.1460-2075.1992.tb05394.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Locht C., Keith J. M. Pertussis toxin gene: nucleotide sequence and genetic organization. Science. 1986 Jun 6;232(4755):1258–1264. doi: 10.1126/science.3704651. [DOI] [PubMed] [Google Scholar]
  22. Maskell D. J., Morrissey P., Dougan G. Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis. J Bacteriol. 1988 Jun;170(6):2467–2471. doi: 10.1128/jb.170.6.2467-2471.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. McClelland M., Jones R., Patel Y., Nelson M. Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 1987 Aug 11;15(15):5985–6005. doi: 10.1093/nar/15.15.5985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mooi F. R., ter Avest A., van der Heide H. G. Structure of the Bordetella pertussis gene coding for the serotype 3 fimbrial subunit. FEMS Microbiol Lett. 1990 Jan 1;54(1-3):327–331. doi: 10.1016/0378-1097(90)90307-c. [DOI] [PubMed] [Google Scholar]
  25. Mooi F. R., van der Heide H. G., ter Avest A. R., Welinder K. G., Livey I., van der Zeijst B. A., Gaastra W. Characterization of fimbrial subunits from Bordetella species. Microb Pathog. 1987 Jun;2(6):473–484. doi: 10.1016/0882-4010(87)90054-4. [DOI] [PubMed] [Google Scholar]
  26. Nicosia A., Perugini M., Franzini C., Casagli M. C., Borri M. G., Antoni G., Almoni M., Neri P., Ratti G., Rappuoli R. Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4631–4635. doi: 10.1073/pnas.83.13.4631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pedroni P., Riboli B., de Ferra F., Grandi G., Toma S., Aricò B., Rappuoli R. Cloning of a novel pilin-like gene from Bordetella pertussis: homology to the fim2 gene. Mol Microbiol. 1988 Jul;2(4):539–543. doi: 10.1111/j.1365-2958.1988.tb00061.x. [DOI] [PubMed] [Google Scholar]
  28. Relman D. A., Domenighini M., Tuomanen E., Rappuoli R., Falkow S. Filamentous hemagglutinin of Bordetella pertussis: nucleotide sequence and crucial role in adherence. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2637–2641. doi: 10.1073/pnas.86.8.2637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Roop R. M., 2nd, Veit H. P., Sinsky R. J., Veit S. P., Hewlett E. L., Kornegay E. T. Virulence factors of Bordetella bronchiseptica associated with the production of infectious atrophic rhinitis and pneumonia in experimentally infected neonatal swine. Infect Immun. 1987 Jan;55(1):217–222. doi: 10.1128/iai.55.1.217-222.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith C. L., Cantor C. R. Purification, specific fragmentation, and separation of large DNA molecules. Methods Enzymol. 1987;155:449–467. doi: 10.1016/0076-6879(87)55030-3. [DOI] [PubMed] [Google Scholar]
  31. Stibitz S., Black W., Falkow S. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene. 1986;50(1-3):133–140. doi: 10.1016/0378-1119(86)90318-5. [DOI] [PubMed] [Google Scholar]
  32. Stibitz S., Weiss A. A., Falkow S. Genetic analysis of a region of the Bordetella pertussis chromosome encoding filamentous hemagglutinin and the pleiotropic regulatory locus vir. J Bacteriol. 1988 Jul;170(7):2904–2913. doi: 10.1128/jb.170.7.2904-2913.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Stibitz S., Yang M. S. Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis. J Bacteriol. 1991 Jul;173(14):4288–4296. doi: 10.1128/jb.173.14.4288-4296.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Weiss A. A., Falkow S. Transposon insertion and subsequent donor formation promoted by Tn501 in Bordetella pertussis. J Bacteriol. 1983 Jan;153(1):304–309. doi: 10.1128/jb.153.1.304-309.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Weiss A. A., Hewlett E. L., Myers G. A., Falkow S. Tn5-induced mutations affecting virulence factors of Bordetella pertussis. Infect Immun. 1983 Oct;42(1):33–41. doi: 10.1128/iai.42.1.33-41.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Weiss A. A., Melton A. R., Walker K. E., Andraos-Selim C., Meidl J. J. Use of the promoter fusion transposon Tn5 lac to identify mutations in Bordetella pertussis vir-regulated genes. Infect Immun. 1989 Sep;57(9):2674–2682. doi: 10.1128/iai.57.9.2674-2682.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Willems R. J., van der Heide H. G., Mooi F. R. Characterization of a Bordetella pertussis fimbrial gene cluster which is located directly downstream of the filamentous haemagglutinin gene. Mol Microbiol. 1992 Sep;6(18):2661–2671. doi: 10.1111/j.1365-2958.1992.tb01443.x. [DOI] [PubMed] [Google Scholar]
  38. Willems R., Paul A., van der Heide H. G., ter Avest A. R., Mooi F. R. Fimbrial phase variation in Bordetella pertussis: a novel mechanism for transcriptional regulation. EMBO J. 1990 Sep;9(9):2803–2809. doi: 10.1002/j.1460-2075.1990.tb07468.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES