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. 1992 Dec;174(24):8057–8064. doi: 10.1128/jb.174.24.8057-8064.1992

Role of PhoU in phosphate transport and alkaline phosphatase regulation.

M Muda 1, N N Rao 1, A Torriani 1
PMCID: PMC207544  PMID: 1459954

Abstract

The negative regulatory function of PhoU in alkaline phosphatase (AP) was suggested by the behavior of K10 phoU35 carrying a missense mutation whose product was detected by immunoblotting. To define more clearly the regulatory function of this protein for the synthesis of AP, we constructed a null mutation. The constitutive synthesis of AP in this phoU deletion strain confirmed the negative role of PhoU. However, the expression of the PhoU protein from an isopropyl-beta-D-thiogalactopyranoside-inducible promoter had no effect on the repression of AP synthesis. Furthermore, the involvement of PhoU in free-Pi uptake was demonstrated. These results provide evidence that PhoU participates in Pi transport and in the regulatory role of the phosphate-specific transport system.

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Selected References

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