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. 1992 Dec;174(24):8139–8143. doi: 10.1128/jb.174.24.8139-8143.1992

Three clustered origins of replication in a promiscuous-plasmid replicon and their differential use in a PolA+ strain and a delta PolA strain of Escherichia coli K-12.

S K Banerjee 1, B T Luck 1, H Y Kim 1, V N Iyer 1
PMCID: PMC207553  PMID: 1459961

Abstract

A 1,197-bp region of the broad-host-range plasmid pCU1 is adequate for its replication. Analysis of replicating molecules containing this region reveals three clustered origins of vegetative replication and replication proceeds bidirectionally from each in a theta mode. In an Escherichia coli polymerase I deletion mutant, utilization of one of these three origins was not detected. The potentiality for origin utilization may therefore be a determinant of replicon host range.

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Selected References

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