Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Dec;174(24):8156–8157. doi: 10.1128/jb.174.24.8156-8157.1992

Presence of methylated adenine in GATC sequences in chromosomal DNAs from Campylobacter species.

P Edmonds 1, B M Hall 1, W R Edwards 1, K M Hartline 1
PMCID: PMC207557  PMID: 1459965

Abstract

We digested chromosomal DNAs from 12 Campylobacter strains (C. jejuni, 4 strains; C. coli, 2 strains; C. fetus subsp. fetus, 2 strains; C. hyointestinalis, 2 strains; and C. upsaliensis, 2 strains) and from 4 Helicobacter strains (H. pylori, 2 strains; and H. mustelae, 2 strains) with HindIII, SstI, BamHI, DpnI, MboI, and Sau3AI. Restriction fragments were then separated by electrophoresis in 1% agarose or 10% polyacrylamide gels. Only DNAs from three Campylobacter species (C. jejuni, C. coli, and C. upsaliensis) were digested with DpnI (an enzyme that recognizes only methylated adenine in GATC sequences). We used MboI and Sau3AI to confirm these findings.

Full text

PDF
8156

Images in this article

8157Image
on p.8157

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bakker A., Smith D. W. Methylation of GATC sites is required for precise timing between rounds of DNA replication in Escherichia coli. J Bacteriol. 1989 Oct;171(10):5738–5742. doi: 10.1128/jb.171.10.5738-5742.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbeyron T., Kean K., Forterre P. DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage. J Bacteriol. 1984 Nov;160(2):586–590. doi: 10.1128/jb.160.2.586-590.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barras F., Marinus M. G. The great GATC: DNA methylation in E. coli. Trends Genet. 1989 May;5(5):139–143. doi: 10.1016/0168-9525(89)90054-1. [DOI] [PubMed] [Google Scholar]
  4. Blaser M. J., Wells J. G., Feldman R. A., Pollard R. A., Allen J. R. Campylobacter enteritis in the United States. A multicenter study. Ann Intern Med. 1983 Mar;98(3):360–365. doi: 10.7326/0003-4819-98-3-360. [DOI] [PubMed] [Google Scholar]
  5. Bok H. E., Greeff A. S., Crewe-Brown H. H. Incidence of toxigenic Campylobacter strains in South Africa. J Clin Microbiol. 1991 Jun;29(6):1262–1264. doi: 10.1128/jcm.29.6.1262-1264.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Boye E. The hemimethylated replication origin of Escherichia coli can be initiated in vitro. J Bacteriol. 1991 Jul;173(14):4537–4539. doi: 10.1128/jb.173.14.4537-4539.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cerritelli S., Springhorn S. S., Lacks S. A. DpnA, a methylase for single-strand DNA in the Dpn II restriction system, and its biological function. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9223–9227. doi: 10.1073/pnas.86.23.9223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deming M. S., Tauxe R. V., Blake P. A., Dixon S. E., Fowler B. S., Jones T. S., Lockamy E. A., Patton C. M., Sikes R. O. Campylobacter enteritis at a university: transmission from eating chicken and from cats. Am J Epidemiol. 1987 Sep;126(3):526–534. doi: 10.1093/oxfordjournals.aje.a114685. [DOI] [PubMed] [Google Scholar]
  9. Dingman D. W. Presence of N6-methyladenine in GATC sequences of Bacillus popilliae and Bacillus lentimorbus KLN2. J Bacteriol. 1990 Oct;172(10):6156–6159. doi: 10.1128/jb.172.10.6156-6159.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Edmonds P., Patton C. M., Barrett T. J., Morris G. K., Steigerwalt A. G., Brenner D. J. Biochemical and genetic characteristics of atypical Campylobacter fetus subsp. fetus strains isolated from humans in the United States. J Clin Microbiol. 1985 Jun;21(6):936–940. doi: 10.1128/jcm.21.6.936-940.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Edmonds P., Patton C. M., Griffin P. M., Barrett T. J., Schmid G. P., Baker C. N., Lambert M. A., Brenner D. J. Campylobacter hyointestinalis associated with human gastrointestinal disease in the United States. J Clin Microbiol. 1987 Apr;25(4):685–691. doi: 10.1128/jcm.25.4.685-691.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gomez-Eichelmann M. C., Levy-Mustri A., Ramirez-Santos J. Presence of 5-methylcytosine in CC(A/T)GG sequences (Dcm methylation) in DNAs from different bacteria. J Bacteriol. 1991 Dec;173(23):7692–7694. doi: 10.1128/jb.173.23.7692-7694.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hughes C. A., Johnson R. C. Methylated DNA in Borrelia species. J Bacteriol. 1990 Nov;172(11):6602–6604. doi: 10.1128/jb.172.11.6602-6604.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kessler C., Höltke H. J. Specificity of restriction endonucleases and methylases--a review. Gene. 1986;47(1):1–153. doi: 10.1016/0378-1119(86)90245-3. [DOI] [PubMed] [Google Scholar]
  15. Lacks S. A., Mannarelli B. M., Springhorn S. S., Greenberg B. Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism. Cell. 1986 Sep 26;46(7):993–1000. doi: 10.1016/0092-8674(86)90698-7. [DOI] [PubMed] [Google Scholar]
  16. MacNeil D. J. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J Bacteriol. 1988 Dec;170(12):5607–5612. doi: 10.1128/jb.170.12.5607-5612.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Paster B. J., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser G. J., O'Rourke J. L., Taylor N. S., Ferrero R. Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int J Syst Bacteriol. 1991 Jan;41(1):31–38. doi: 10.1099/00207713-41-1-31. [DOI] [PubMed] [Google Scholar]
  18. Patton C. M., Shaffer N., Edmonds P., Barrett T. J., Lambert M. A., Baker C., Perlman D. M., Brenner D. J. Human disease associated with "Campylobacter upsaliensis" (catalase-negative or weakly positive Campylobacter species) in the United States. J Clin Microbiol. 1989 Jan;27(1):66–73. doi: 10.1128/jcm.27.1.66-73.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Penner J. L. The genus Campylobacter: a decade of progress. Clin Microbiol Rev. 1988 Apr;1(2):157–172. doi: 10.1128/cmr.1.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1985;13 (Suppl):r165–r200. doi: 10.1093/nar/13.suppl.r165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol. 1991 Jan;41(1):88–103. doi: 10.1099/00207713-41-1-88. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES