Abstract
In the circular plasmid pKD1, which stably replicates in Kluyveromyces lactis, the three open reading frames encode a site-specific recombinase (gene A) and two proteins involved in mitotic stability (genes B and C). A recombination analysis of plasmids in which gene B or C is inactivated reveals that unlike the 2 microns plasmid of Saccharomyces cerevisiae, these genes are also required for the site specificity of plasmid recombination.
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- Babineau D., Vetter D., Andrews B. J., Gronostajski R. M., Proteau G. A., Beatty L. G., Sadowski P. D. The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene. J Biol Chem. 1985 Oct 5;260(22):12313–12319. [PubMed] [Google Scholar]
- Bianchi M. M., Santarelli R., Frontali L. Plasmid functions involved in the stable propagation of the pKD1 circular plasmid in Kluyveromyces lactis. Curr Genet. 1991 Mar;19(3):155–161. doi: 10.1007/BF00336481. [DOI] [PubMed] [Google Scholar]
- Broach J. R., Guarascio V. R., Jayaram M. Recombination within the yeast plasmid 2mu circle is site-specific. Cell. 1982 May;29(1):227–234. doi: 10.1016/0092-8674(82)90107-6. [DOI] [PubMed] [Google Scholar]
- Chen X. J., Bianchi M. M., Suda K., Fukuhara H. The host range of the pKD1-derived plasmids in yeast. Curr Genet. 1989 Aug;16(2):95–98. doi: 10.1007/BF00393401. [DOI] [PubMed] [Google Scholar]
- Falcone C., Saliola M., Chen X. J., Frontali L., Fukuhara H. Analysis of a 1.6-micron circular plasmid from the yeast Kluyveromyces drosophilarum: structure and molecular dimorphism. Plasmid. 1986 May;15(3):248–252. doi: 10.1016/0147-619x(86)90044-2. [DOI] [PubMed] [Google Scholar]
- Futcher A. B. Copy number amplification of the 2 micron circle plasmid of Saccharomyces cerevisiae. J Theor Biol. 1986 Mar 21;119(2):197–204. doi: 10.1016/s0022-5193(86)80074-1. [DOI] [PubMed] [Google Scholar]
- Kikuchi Y. Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance. Cell. 1983 Dec;35(2 Pt 1):487–493. doi: 10.1016/0092-8674(83)90182-4. [DOI] [PubMed] [Google Scholar]
- Murray J. A., Scarpa M., Rossi N., Cesareni G. Antagonistic controls regulate copy number of the yeast 2 mu plasmid. EMBO J. 1987 Dec 20;6(13):4205–4212. doi: 10.1002/j.1460-2075.1987.tb02768.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sadowski P. Site-specific recombinases: changing partners and doing the twist. J Bacteriol. 1986 Feb;165(2):341–347. doi: 10.1128/jb.165.2.341-347.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Som T., Armstrong K. A., Volkert F. C., Broach J. R. Autoregulation of 2 micron circle gene expression provides a model for maintenance of stable plasmid copy levels. Cell. 1988 Jan 15;52(1):27–37. doi: 10.1016/0092-8674(88)90528-4. [DOI] [PubMed] [Google Scholar]
- Veit B. E., Fangman W. L. Copy number and partition of the Saccharomyces cerevisiae 2 micron plasmid controlled by transcription regulators. Mol Cell Biol. 1988 Nov;8(11):4949–4957. doi: 10.1128/mcb.8.11.4949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Volkert F. C., Broach J. R. Site-specific recombination promotes plasmid amplification in yeast. Cell. 1986 Aug 15;46(4):541–550. doi: 10.1016/0092-8674(86)90879-2. [DOI] [PubMed] [Google Scholar]
- Volkert F. C., Wilson D. W., Broach J. R. Deoxyribonucleic acid plasmids in yeasts. Microbiol Rev. 1989 Sep;53(3):299–317. doi: 10.1128/mr.53.3.299-317.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wesolowski-Louvel M., Tanguy-Rougeau C., Fukuhara H. A nuclear gene required for the expression of the linear DNA-associated killer system in the yeast Kluyveromyces lactis. Yeast. 1988 Mar;4(1):71–81. doi: 10.1002/yea.320040108. [DOI] [PubMed] [Google Scholar]