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. 1992 Oct;174(19):6198–6206. doi: 10.1128/jb.174.19.6198-6206.1992

A three-start helical sheath on the flagellar filament of Caulobacter crescentus.

S Trachtenberg 1, D J DeRosier 1
PMCID: PMC207688  PMID: 1400169

Abstract

An unusual feature in preparations of the Caulobacter crescentus flagellar filaments is that some filaments are surrounded by a set of three windings that form a sheath. We provide evidence that the sheath is composed of subunits having a molecular mass of 24,000 Da. We suggest that the sheath could be composed of protofilaments of flagellin wound around the filament.

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  1. ABRAM D., KOFFLER H. IN VITRO FORMATION OF FLAGELLA-LIKE FILAMENTS AND OTHER STRUCTURES FROM FLAGELLIN. J Mol Biol. 1964 Jul;9:168–185. doi: 10.1016/s0022-2836(64)80098-x. [DOI] [PubMed] [Google Scholar]
  2. Abram D., Vatter A. E., Koffler H. Attachment and structural features of flagella of certain bacilli. J Bacteriol. 1966 May;91(5):2045–2068. doi: 10.1128/jb.91.5.2045-2068.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Charon N. W., Goldstein S. F., Curci K., Limberger R. J. The bent-end morphology of Treponema phagedenis is associated with short, left-handed, periplasmic flagella. J Bacteriol. 1991 Aug;173(15):4820–4826. doi: 10.1128/jb.173.15.4820-4826.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeRosier D. J., Moore P. B. Reconstruction of three-dimensional images from electron micrographs of structures with helical symmetry. J Mol Biol. 1970 Sep 14;52(2):355–369. doi: 10.1016/0022-2836(70)90036-7. [DOI] [PubMed] [Google Scholar]
  5. Driks A., Bryan R., Shapiro L., DeRosier D. J. The organization of the Caulobacter crescentus flagellar filament. J Mol Biol. 1989 Apr 20;206(4):627–636. doi: 10.1016/0022-2836(89)90571-8. [DOI] [PubMed] [Google Scholar]
  6. GLAUERT A. M., KERRIDGE D., HORNE R. W. THE FINE STRUCTURE AND MODE OF ATTACHMENT OF THE SHEATHED FLAGELLUM OF VIBRIO METCHNIKOVII. J Cell Biol. 1963 Aug;18:327–336. doi: 10.1083/jcb.18.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gill P. R., Agabian N. The nucleotide sequence of the Mr = 28,500 flagellin gene of Caulobacter crescentus. J Biol Chem. 1983 Jun 25;258(12):7395–7401. [PubMed] [Google Scholar]
  8. Hainfeld J. F., Wall J. S., Desmond E. J. A small computer system for micrograph analysis. Ultramicroscopy. 1982;8(3):263–270. doi: 10.1016/0304-3991(82)90242-x. [DOI] [PubMed] [Google Scholar]
  9. Hranitzky K. W., Mulholland A., Larson A. D., Eubanks E. R., Hart L. T. Characterization of a flagellar sheath protein of Vibrio cholerae. Infect Immun. 1980 Feb;27(2):597–603. doi: 10.1128/iai.27.2.597-603.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hyman H. C., Trachtenberg S. Point mutations that lock Salmonella typhimurium flagellar filaments in the straight right-handed and left-handed forms and their relation to filament superhelicity. J Mol Biol. 1991 Jul 5;220(1):79–88. doi: 10.1016/0022-2836(91)90382-g. [DOI] [PubMed] [Google Scholar]
  11. Johnson R. C., Ferber D. M., Ely B. Synthesis and assembly of flagellar components by Caulobacter crescentus motility mutants. J Bacteriol. 1983 Jun;154(3):1137–1144. doi: 10.1128/jb.154.3.1137-1144.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones G. W., Freter R. Adhesive properties of Vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infect Immun. 1976 Jul;14(1):240–245. doi: 10.1128/iai.14.1.240-245.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kanto S., Okino H., Aizawa S., Yamaguchi S. Amino acids responsible for flagellar shape are distributed in terminal regions of flagellin. J Mol Biol. 1991 Jun 5;219(3):471–480. doi: 10.1016/0022-2836(91)90187-b. [DOI] [PubMed] [Google Scholar]
  14. Krupski G., Götz R., Ober K., Pleier E., Schmitt R. Structure of complex flagellar filaments in Rhizobium meliloti. J Bacteriol. 1985 Apr;162(1):361–366. doi: 10.1128/jb.162.1.361-366.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuwajima G. Construction of a minimum-size functional flagellin of Escherichia coli. J Bacteriol. 1988 Jul;170(7):3305–3309. doi: 10.1128/jb.170.7.3305-3309.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWY J., HANSON J. ELECTRON MICROSCOPE STUDIES OF BACTERIAL FLAGELLA. J Mol Biol. 1965 Feb;11:293–313. doi: 10.1016/s0022-2836(65)80059-6. [DOI] [PubMed] [Google Scholar]
  17. LOWY J., HANSON J. STRUCTURE OF BACTERIAL FLAGELLA. Nature. 1964 May 9;202:538–540. doi: 10.1038/202538a0. [DOI] [PubMed] [Google Scholar]
  18. Lagenaur C., Agabian N. Physical characterization of Caulobacter crescentus flagella. J Bacteriol. 1976 Oct;128(1):435–444. doi: 10.1128/jb.128.1.435-444.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lawn A. M. Comparison of the flagellins from different flagellar morphotypes of Escherichia coli. J Gen Microbiol. 1977 Jul;101(1):112–130. doi: 10.1099/00221287-101-1-121. [DOI] [PubMed] [Google Scholar]
  20. Lawn A. M., Orskov I., Orskov F. Morphological distinction between different H serotypes of Escherichia coli. J Gen Microbiol. 1977 Jul;101(1):111–119. doi: 10.1099/00221287-101-1-111. [DOI] [PubMed] [Google Scholar]
  21. Lotz W., Acker G., Schmitt R. Bacteriophage 7-7-1 adsorbs to the complex flagella of Rhizobium lupini H13-3. J Gen Virol. 1977 Jan;34(1):9–17. doi: 10.1099/0022-1317-34-1-9. [DOI] [PubMed] [Google Scholar]
  22. Maruyama M., Lodderstaedt G., Schmitt R. Purification and biochemical properties of complex flagella isolated from Rhizobium lupini H13-3. Biochim Biophys Acta. 1978 Jul 21;535(1):110–124. doi: 10.1016/0005-2795(78)90038-7. [DOI] [PubMed] [Google Scholar]
  23. Meadows P. S. The attachment of bacteria to solid surfaces. Arch Mikrobiol. 1971;75(4):374–381. doi: 10.1007/BF00407699. [DOI] [PubMed] [Google Scholar]
  24. Minnich S. A., Newton A. Promoter mapping and cell cycle regulation of flagellin gene transcription in Caulobacter crescentus. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1142–1146. doi: 10.1073/pnas.84.5.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Namba K., Yamashita I., Vonderviszt F. Structure of the core and central channel of bacterial flagella. Nature. 1989 Dec 7;342(6250):648–654. doi: 10.1038/342648a0. [DOI] [PubMed] [Google Scholar]
  26. Newton A., Ohta N. Regulation of the cell division cycle and differentiation in bacteria. Annu Rev Microbiol. 1990;44:689–719. doi: 10.1146/annurev.mi.44.100190.003353. [DOI] [PubMed] [Google Scholar]
  27. Norris S. J., Charon N. W., Cook R. G., Fuentes M. D., Limberger R. J. Antigenic relatedness and N-terminal sequence homology define two classes of periplasmic flagellar proteins of Treponema pallidum subsp. pallidum and Treponema phagedenis. J Bacteriol. 1988 Sep;170(9):4072–4082. doi: 10.1128/jb.170.9.4072-4082.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. O'Brien E. J., Bennett P. M. Structure of straight flagella from a mutant Salmonella. J Mol Biol. 1972 Sep 14;70(1):133–152. doi: 10.1016/0022-2836(72)90168-4. [DOI] [PubMed] [Google Scholar]
  29. Schmitt R., Raska I., Mayer F. Plain and complex flagella of Pseudomonas rhodos: analysis of fine structure and composition. J Bacteriol. 1974 Feb;117(2):844–857. doi: 10.1128/jb.117.2.844-857.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Shirakihara Y., Wakabayashi T. Three-dimensional image reconstruction of straight flagella from a mutant Salmonella typhimurium. J Mol Biol. 1979 Jul 5;131(3):485–507. doi: 10.1016/0022-2836(79)90004-4. [DOI] [PubMed] [Google Scholar]
  31. Sjoblad R. D., Emala C. W., Doetsch R. N. Invited review: bacterial flagellar sheaths: structures in search of a function. Cell Motil. 1983;3(1):93–103. doi: 10.1002/cm.970030108. [DOI] [PubMed] [Google Scholar]
  32. Trachtenberg S., DeRosier D. J. A molecular switch: subunit rotations involved in the right-handed to left-handed transitions of Salmonella typhimurium flagellar filaments. J Mol Biol. 1991 Jul 5;220(1):67–77. doi: 10.1016/0022-2836(91)90381-f. [DOI] [PubMed] [Google Scholar]
  33. Trachtenberg S., DeRosier D. J., Aizawa S., Macnab R. M. Pairwise perturbation of flagellin subunits. The structural basis for the differences between plain and complex bacterial flagellar filaments. J Mol Biol. 1986 Aug 20;190(4):569–576. doi: 10.1016/0022-2836(86)90242-1. [DOI] [PubMed] [Google Scholar]
  34. Trachtenberg S., DeRosier D. J., Macnab R. M. Three-dimensional structure of the complex flagellar filament of Rhizobium lupini and its relation to the structure of the plain filament. J Mol Biol. 1987 Jun 5;195(3):603–620. doi: 10.1016/0022-2836(87)90185-9. [DOI] [PubMed] [Google Scholar]
  35. Trachtenberg S., DeRosier D. J. Three-dimensional reconstruction of the flagellar filament of Caulobacter crescentus. A flagellin lacking the outer domain and its amino acid sequence lacking an internal segment. J Mol Biol. 1988 Aug 20;202(4):787–808. doi: 10.1016/0022-2836(88)90559-1. [DOI] [PubMed] [Google Scholar]
  36. Trachtenberg S., DeRosier D. J. Three-dimensional structure of the frozen-hydrated flagellar filament. The left-handed filament of Salmonella typhimurium. J Mol Biol. 1987 Jun 5;195(3):581–601. doi: 10.1016/0022-2836(87)90184-7. [DOI] [PubMed] [Google Scholar]
  37. Trachtenberg S., Hammel I. The rigidity of bacterial flagellar filaments and its relation to filament polymorphism. J Struct Biol. 1992 Jul-Aug;109(1):18–27. doi: 10.1016/1047-8477(92)90063-g. [DOI] [PubMed] [Google Scholar]
  38. Wagenknecht T., DeRosier D. J., Aizawa S., Macnab R. M. Flagellar hook structures of Caulobacter and Salmonella and their relationship to filament structure. J Mol Biol. 1982 Nov 25;162(1):69–87. doi: 10.1016/0022-2836(82)90162-0. [DOI] [PubMed] [Google Scholar]
  39. Wagenknecht T., DeRosier D., Shapiro L., Weissborn A. Three-dimensional reconstruction of the flagellar hook from Caulobacter crescentus. J Mol Biol. 1981 Sep 25;151(3):439–465. doi: 10.1016/0022-2836(81)90005-x. [DOI] [PubMed] [Google Scholar]
  40. Wall J. S., Hainfeld J. F. Mass mapping with the scanning transmission electron microscope. Annu Rev Biophys Biophys Chem. 1986;15:355–376. doi: 10.1146/annurev.bb.15.060186.002035. [DOI] [PubMed] [Google Scholar]
  41. Weissborn A., Steinmann H. M., Shapiro L. Characterization of the proteins of the Caulobacter crescentus flagellar filament. Peptide analysis and filament organization. J Biol Chem. 1982 Feb 25;257(4):2066–2074. [PubMed] [Google Scholar]
  42. Yang G. C., Schrank G. D., Freeman B. A. Purification of flagellar cores of Vibrio cholerae. J Bacteriol. 1977 Feb;129(2):1121–1128. doi: 10.1128/jb.129.2.1121-1128.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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