Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Oct;174(19):6290–6293. doi: 10.1128/jb.174.19.6290-6293.1992

Maturation of membrane-bound hydrogenase of Alcaligenes eutrophus H16.

C Kortlüke 1, B Friedrich 1
PMCID: PMC207699  PMID: 1400179

Abstract

The formation of the catalytically active membrane-bound hydrogenase (MBH) of Alcaligenes eutrophus H16 requires the genes for the small and large subunits of the enzyme (hoxK and hoxG, respectively) and an accompanying set of accessory genes (C. Kortl ke, K. Horstmann, E. Schwartz, M. Rohde, R. Binsack, and B. Friedrich, J. Bacteriol. 174:6277-6289, 1992). Other genes located in the adjacent pleiotropic region are also required. In the absence of these genes, MBH is synthesized but is catalytically inactive. Immunological analyses revealed that cells containing active MBH produced the small and large subunits of the enzyme in two distinct conformations each; only one of each, presumably the immature form, occurred in cells devoid of MBH activity. The results suggest that the conversion of the two subunits into the catalytically active membrane-associated heterodimer depends on specific maturation processes mediated by hox genes.

Full text

PDF
6290

Images in this article

6292Image
on p.6292

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowien B., Schlegel H. G. Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annu Rev Microbiol. 1981;35:405–452. doi: 10.1146/annurev.mi.35.100181.002201. [DOI] [PubMed] [Google Scholar]
  2. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  3. Ditta G., Stanfield S., Corbin D., Helinski D. R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. doi: 10.1073/pnas.77.12.7347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eberz G., Eitinger T., Friedrich B. Genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in Alcaligenes eutrophus. J Bacteriol. 1989 Mar;171(3):1340–1345. doi: 10.1128/jb.171.3.1340-1345.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eberz G., Friedrich B. Three trans-acting regulatory functions control hydrogenase synthesis in Alcaligenes eutrophus. J Bacteriol. 1991 Mar;173(6):1845–1854. doi: 10.1128/jb.173.6.1845-1854.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eberz G., Hogrefe C., Kortlüke C., Kamienski A., Friedrich B. Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16. J Bacteriol. 1986 Nov;168(2):636–641. doi: 10.1128/jb.168.2.636-641.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedrich B., Heine E., Finck A., Friedrich C. G. Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus. J Bacteriol. 1981 Mar;145(3):1144–1149. doi: 10.1128/jb.145.3.1144-1149.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friedrich C. G. Depression of hydrogenase during limitation of electron donors and derepression of ribulosebisphosphate carboxylase during carbon limitation of Alcaligenes eutrophus. J Bacteriol. 1982 Jan;149(1):203–210. doi: 10.1128/jb.149.1.203-210.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hogrefe C., Römermann D., Friedrich B. Alcaligenes eutrophus hydrogenase genes (Hox). J Bacteriol. 1984 Apr;158(1):43–48. doi: 10.1128/jb.158.1.43-48.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knauf V. C., Nester E. W. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid. 1982 Jul;8(1):45–54. doi: 10.1016/0147-619x(82)90040-3. [DOI] [PubMed] [Google Scholar]
  11. Kortlüke C., Horstmann K., Schwartz E., Rohde M., Binsack R., Friedrich B. A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16. J Bacteriol. 1992 Oct;174(19):6277–6289. doi: 10.1128/jb.174.19.6277-6289.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lorenz B., Schneider K., Kratzin H., Schlegel H. G. Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria. Biochim Biophys Acta. 1989 Mar 16;995(1):1–9. doi: 10.1016/0167-4838(89)90225-2. [DOI] [PubMed] [Google Scholar]
  13. Lutz S., Jacobi A., Schlensog V., Böhm R., Sawers G., Böck A. Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli. Mol Microbiol. 1991 Jan;5(1):123–135. doi: 10.1111/j.1365-2958.1991.tb01833.x. [DOI] [PubMed] [Google Scholar]
  14. Menon N. K., Peck H. D., Jr, Gall J. L., Przybyla A. E. Cloning and sequencing of the genes encoding the large and small subunits of the periplasmic (NiFeSe) hydrogenase of Desulfovibrio baculatus. J Bacteriol. 1987 Dec;169(12):5401–5407. doi: 10.1128/jb.169.12.5401-5407.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SCHLEGEL H. G., KALTWASSER H., GOTTSCHALK G. [A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies]. Arch Mikrobiol. 1961;38:209–222. [PubMed] [Google Scholar]
  16. Schink B., Schlegel H. G. The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties. Biochim Biophys Acta. 1979 Apr 12;567(2):315–324. doi: 10.1016/0005-2744(79)90117-7. [DOI] [PubMed] [Google Scholar]
  17. Srivastava S., Urban M., Friedrich B. Mutagenesis of Alcaligenes eutrophus by insertion of the drug-resistance transposon Tn5. Arch Microbiol. 1982 May;131(3):203–207. doi: 10.1007/BF00405879. [DOI] [PubMed] [Google Scholar]
  18. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES