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. 1996 Dec;74(11):1828–1833. doi: 10.1038/bjc.1996.638

Characterisation of immune responses in pancreatic carcinoma patients after mutant p21 ras peptide vaccination.

M K Gjertsen 1, I Saeterdal 1, E Thorsby 1, G Gaudernack 1
PMCID: PMC2077214  PMID: 8956801

Abstract

This is a study of immune responses generated by mutant ras peptide vaccination of patients with pancreatic adenocarcinoma. Responding T cells from one patient were cloned and two CD4+ T-lymphocyte clones (TLC) specific for the 12 Val peptide and restricted by HLA-DR6 or DQ2 were obtained. These class II molecules have not previously been found to bind or present mutant ras peptides to T cells. The DR6-restricted TLC showed marked cytotoxicity against autologous target cells pulsed with the 12 Val peptide. Target cells pulsed with the control peptide were not killed. Responding T cells from another patient showed cross-reactivity towards the homologous ras peptides. Investigation by limiting dilution analysis (LDA) revealed different T-cell precursor frequencies for the immunising, mutant ras peptide (1:28000), compared with the normal ras peptide (1:110000).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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