Abstract
A DNA fragment containing a portion of a DNA damage-inducible gene from Deinococcus radiodurans SARK hybridized to numerous fragments of SARK genomic DNA because of a highly conserved repetitive chromosomal element. The element is of variable length, ranging from 150 to 192 bp, depending on the absence or presence of one or two 21-bp sequences located internally. A putative translational start site of the damage-inducible gene is within the reiterated element. The element contains dyad symmetries that suggest modes of transcriptional and/or translational control.
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- Brendel V., Trifonov E. N. A computer algorithm for testing potential prokaryotic terminators. Nucleic Acids Res. 1984 May 25;12(10):4411–4427. doi: 10.1093/nar/12.10.4411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lennon E., Minton K. W. Gene fusions with lacZ by duplication insertion in the radioresistant bacterium Deinococcus radiodurans. J Bacteriol. 1990 Jun;172(6):2955–2961. doi: 10.1128/jb.172.6.2955-2961.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson G. W., Nicolet C. M., Kalainov D., Friedberg E. C. A yeast excision-repair gene is inducible by DNA damaging agents. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1842–1846. doi: 10.1073/pnas.83.6.1842. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith M. D., Abrahamson R., Minton K. W. Shuttle plasmids constructed by the transformation of an Escherichia coli cloning vector into two Deinococcus radiodurans plasmids. Plasmid. 1989 Sep;22(2):132–142. doi: 10.1016/0147-619x(89)90022-x. [DOI] [PubMed] [Google Scholar]
- Smith M. D., Lennon E., McNeil L. B., Minton K. W. Duplication insertion of drug resistance determinants in the radioresistant bacterium Deinococcus radiodurans. J Bacteriol. 1988 May;170(5):2126–2135. doi: 10.1128/jb.170.5.2126-2135.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]