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. 1991 Apr;173(7):2297–2300. doi: 10.1128/jb.173.7.2297-2300.1991

Pleiotropic effects of a relC mutation in Streptomyces antibioticus.

K S Kelly 1, K Ochi 1, G H Jones 1
PMCID: PMC207782  PMID: 1706704

Abstract

Ochi (Agric. Biol. Chem. 51:829-835, 1987) has isolated a relaxed mutant of Streptomyces antibioticus, designated relC49, relC49 accumulates significantly lower levels of ppGpp than the parent stain, IMRU3720. At its maximum, the ppGpp level in relC49 was only one-fourth that observed in strain IMRU3720. Interestingly, a burst of ppGpp synthesis between 18 and 22 h of growth in IMRU3720 coincided with the onset of actinomycin production in that strain. As shown previously, the activity in protein synthesis of ribosomes from strain IMRU3720 decreases with the age of the culture. The decrease in activity was less pronounced in cultures of relC49. relC49 mycelium contains reduced levels of phenoxazinone synthase, a key enzyme involved in actinomycin biosynthesis. The rel mutation prevents the normal increase in the activity of one of the other enzymes required for production of the antibiotic, 3-hydroxyanthanilate-4-methyltransferase, and a third enzyme, actinomycin synthetase I, appears to be completely absent from relC49 mycelium. Levels of phenoxazinone synthease mRNA were examined by RNA dot blotting with the cloned phenoxazinone synthase gene as a probe. mRNA levels for phenoxazinone synthase were dramatically reduced in relC49 compared with strain IMRU3720. These results are discussed in terms of the possible regulation of the onset of actinomycin production by ppGpp.

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Selected References

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  1. Baracchini E., Glass R., Bremer H. Studies in vivo on Escherichia coli RNA polymerase mutants altered in the stringent response. Mol Gen Genet. 1988 Aug;213(2-3):379–387. doi: 10.1007/BF00339606. [DOI] [PubMed] [Google Scholar]
  2. Chater K. F. Multilevel regulation of Streptomyces differentiation. Trends Genet. 1989 Nov;5(11):372–377. doi: 10.1016/0168-9525(89)90172-8. [DOI] [PubMed] [Google Scholar]
  3. Choy H. A., Jones G. H. Phenoxazinone synthase from Streptomyces antibiotics: purification of the large and small enzyme forms. Arch Biochem Biophys. 1981 Oct 1;211(1):55–65. doi: 10.1016/0003-9861(81)90429-x. [DOI] [PubMed] [Google Scholar]
  4. Fawaz F., Jones G. H. Actinomycin synthesis in Streptomyces antibioticus. Purification and properties of a 3-hydroxyanthranilate 4-methyltransferase. J Biol Chem. 1988 Apr 5;263(10):4602–4606. [PubMed] [Google Scholar]
  5. Jones G. H., Hopwood D. A. Molecular cloning and expression of the phenoxazinone synthase gene from Streptomyces antibioticus. J Biol Chem. 1984 Nov 25;259(22):14151–14157. [PubMed] [Google Scholar]
  6. Jones G. H. Macromolecular synthesis in Streptomyces antibioticus: in vitro systems for aminoacylation and translation from young and old cells. J Bacteriol. 1975 Oct;124(1):364–372. doi: 10.1128/jb.124.1.364-372.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones G. H. Marcaine, a selective inhibitor of eucaryotic aminoacylation. Biochemistry. 1979 Oct 16;18(21):4542–4547. doi: 10.1021/bi00588a014. [DOI] [PubMed] [Google Scholar]
  8. Jones G. H. Regulation of phenoxazinone synthase expression in Streptomyces antibioticus. J Bacteriol. 1985 Sep;163(3):1215–1221. doi: 10.1128/jb.163.3.1215-1221.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kajitani M., Ishihama A. Promoter selectivity of Escherichia coli RNA polymerase. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons. J Biol Chem. 1984 Feb 10;259(3):1951–1957. [PubMed] [Google Scholar]
  10. Keller U. Actinomycin synthetases. Multifunctional enzymes responsible for the synthesis of the peptide chains of actinomycin. J Biol Chem. 1987 Apr 25;262(12):5852–5856. [PubMed] [Google Scholar]
  11. Keller U., Kleinkauf H., Zocher R. 4-Methyl-3-hydroxyanthranilic acid activating enzyme from actinomycin-producing Streptomyces chrysomallus. Biochemistry. 1984 Mar 27;23(7):1479–1484. doi: 10.1021/bi00302a022. [DOI] [PubMed] [Google Scholar]
  12. Nagase T., Ishii S., Imamoto F. Differential transcriptional control of the two tRNA(fMet) genes of Escherichia coli K-12. Gene. 1988 Jul 15;67(1):49–57. doi: 10.1016/0378-1119(88)90007-8. [DOI] [PubMed] [Google Scholar]
  13. Ochi K. Metabolic initiation of differentiation and secondary metabolism by Streptomyces griseus: significance of the stringent response (ppGpp) and GTP content in relation to A factor. J Bacteriol. 1987 Aug;169(8):3608–3616. doi: 10.1128/jb.169.8.3608-3616.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ochi K. Occurrence of the stringent response in Streptomyces sp. and its significance for the initiation of morphological and physiological differentiation. J Gen Microbiol. 1986 Sep;132(9):2621–2631. doi: 10.1099/00221287-132-9-2621. [DOI] [PubMed] [Google Scholar]
  15. Ochi K. Streptomyces relC mutants with an altered ribosomal protein ST-L11 and genetic analysis of a Streptomyces griseus relC mutant. J Bacteriol. 1990 Jul;172(7):4008–4016. doi: 10.1128/jb.172.7.4008-4016.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vara J., Perez-Gonzalez J. A., Jimenez A. Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase. Biochemistry. 1985 Dec 31;24(27):8074–8081. doi: 10.1021/bi00348a036. [DOI] [PubMed] [Google Scholar]

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