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. 1991 Apr;173(8):2473–2480. doi: 10.1128/jb.173.8.2473-2480.1991

A novel L-serine deaminase activity in Escherichia coli K-12.

H Su 1, E B Newman 1
PMCID: PMC207810  PMID: 2013569

Abstract

We demonstrate here that Escherichia coli K-12 synthesizes two different L-serine deaminases (L-SD) catalyzing the nonoxidative deamination of L-serine to pyruvate, one coded for by the previously described sdaA gene and a second, hitherto undescribed enzyme which we call L-SD2. A strain carrying a null mutation in sdaA made no detectable L-SD in minimal medium, but had activity in Luria broth. We describe a mutation, sdaX, which affects the regulation of L-SD2 and permits its expression in minimal medium, and an insertion mutation, sdaB, which abolishes L-SD2 activity completely. Both mutations lie near 60.5 min on the E. coli genetic map. The two L-SD enzymes have similar enzyme parameters, and both require posttranslational activation.

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Selected References

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  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990 Jun;54(2):130–197. doi: 10.1128/mr.54.2.130-197.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  3. Bremer E., Silhavy T. J., Weinstock G. M. Transposable lambda placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli. J Bacteriol. 1985 Jun;162(3):1092–1099. doi: 10.1128/jb.162.3.1092-1099.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark R. L., Neidhardt F. C. Roles of the two lysyl-tRNA synthetases of Escherichia coli: analysis of nucleotide sequences and mutant behavior. J Bacteriol. 1990 Jun;172(6):3237–3243. doi: 10.1128/jb.172.6.3237-3243.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cosloy S. D., McFall E. L-Serine-sensitive mutants of Escherichia coli K-12. J Bacteriol. 1970 Sep;103(3):840–841. doi: 10.1128/jb.103.3.840-841.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doy C. H., Brown K. D. Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W. Biochim Biophys Acta. 1965 Jul 8;104(2):377–389. doi: 10.1016/0304-4165(65)90343-0. [DOI] [PubMed] [Google Scholar]
  8. Egan R. M., Phillips A. T. Requirements for induction of the biodegradative threonine dehydratase in Escherichia coli. J Bacteriol. 1977 Nov;132(2):370–376. doi: 10.1128/jb.132.2.370-376.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goss T. J., Datta P. Escherichia coli K-12 mutation that inactivates biodegradative threonine dehydratase by transposon Tn5 insertion. J Bacteriol. 1984 Jun;158(3):826–831. doi: 10.1128/jb.158.3.826-831.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greene R. C., Hunter J. S., Coch E. H. Properties of metK mutants of Escherichia coli K-12. J Bacteriol. 1973 Jul;115(1):57–67. doi: 10.1128/jb.115.1.57-67.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Groisman E. A., Casadaban M. J. Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J Bacteriol. 1986 Oct;168(1):357–364. doi: 10.1128/jb.168.1.357-364.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Groisman E. A., Castilho B. A., Casadaban M. J. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1480–1483. doi: 10.1073/pnas.81.5.1480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hirshfield I. N., Bloch P. L., Van Bogelen R. A., Neidhardt F. C. Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli. J Bacteriol. 1981 Apr;146(1):345–351. doi: 10.1128/jb.146.1.345-351.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Isenberg S., Newman E. B. Studies on L-serine deaminase in Escherichia coli K-12. J Bacteriol. 1974 Apr;118(1):53–58. doi: 10.1128/jb.118.1.53-58.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  16. Newman E. B., Dumont D., Walker C. In vitro and in vivo activation of L-serine deaminase in Escherichia coli K-12. J Bacteriol. 1985 Jun;162(3):1270–1275. doi: 10.1128/jb.162.3.1270-1275.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Newman E. B., Malik N., Walker C. L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants. J Bacteriol. 1982 May;150(2):710–715. doi: 10.1128/jb.150.2.710-715.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Newman E. B., Miller B., Colebrook L. D., Walker C. A mutation in Escherichia coli K-12 results in a requirement for thiamine and a decrease in L-serine deaminase activity. J Bacteriol. 1985 Jan;161(1):272–276. doi: 10.1128/jb.161.1.272-276.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Newman E. B., Morris J. F., Walker C., Kapoor V. A mutation affecting L-serine and energy metabolism in E. coli K12. Mol Gen Genet. 1981;182(1):143–147. doi: 10.1007/BF00422781. [DOI] [PubMed] [Google Scholar]
  20. PARDEE A. B., PRESTIDGE L. S. Induced formation of serine and threonine deaminases by Escherichia coli. J Bacteriol. 1955 Dec;70(6):667–674. doi: 10.1128/jb.70.6.667-674.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Russel M., Holmgren A. Construction and characterization of glutaredoxin-negative mutants of Escherichia coli. Proc Natl Acad Sci U S A. 1988 Feb;85(4):990–994. doi: 10.1073/pnas.85.4.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Satishchandran C., Taylor J. C., Markham G. D. Novel Escherichia coli K-12 mutants impaired in S-adenosylmethionine synthesis. J Bacteriol. 1990 Aug;172(8):4489–4496. doi: 10.1128/jb.172.8.4489-4496.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Singer M., Baker T. A., Schnitzler G., Deischel S. M., Goel M., Dove W., Jaacks K. J., Grossman A. D., Erickson J. W., Gross C. A. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev. 1989 Mar;53(1):1–24. doi: 10.1128/mr.53.1.1-24.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Su H. S., Lang B. F., Newman E. B. L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene. J Bacteriol. 1989 Sep;171(9):5095–5102. doi: 10.1128/jb.171.9.5095-5102.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tuan L. R., D'Ari R., Newman E. B. The leucine regulon of Escherichia coli K-12: a mutation in rblA alters expression of L-leucine-dependent metabolic operons. J Bacteriol. 1990 Aug;172(8):4529–4535. doi: 10.1128/jb.172.8.4529-4535.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Umbarger H. E. Threonine deaminases. Adv Enzymol Relat Areas Mol Biol. 1973;37:349–395. doi: 10.1002/9780470122822.ch6. [DOI] [PubMed] [Google Scholar]
  27. Uzan M., Danchin A. Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants. Mol Gen Genet. 1978 Sep 20;165(1):21–30. doi: 10.1007/BF00270372. [DOI] [PubMed] [Google Scholar]
  28. Winans S. C., Elledge S. J., Krueger J. H., Walker G. C. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli. J Bacteriol. 1985 Mar;161(3):1219–1221. doi: 10.1128/jb.161.3.1219-1221.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wood J. M. Genetics of L-proline utilization in Escherichia coli. J Bacteriol. 1981 Jun;146(3):895–901. doi: 10.1128/jb.146.3.895-901.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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