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. 1991 Apr;173(8):2712–2715. doi: 10.1128/jb.173.8.2712-2715.1991

Molecular cloning of the 5-aminolevulinic acid dehydratase gene from Rhodobacter sphaeroides.

A M Delaunay 1, C Huault 1, A P Balangé 1
PMCID: PMC207843  PMID: 2013584

Abstract

A hemB mutant of Escherichia coli was used to clone the gene encoding 5-aminolevulinic acid dehydratase from Rhodobacter sphaeroides after physiological complementation of the mutation. A 2.9-kb DNA fragment was obtained and cloned in both orientations into the unique PstI restriction site of pUC19. This recombinant plasmid encodes a protein (Mr 39,000) that is immunoreactive with antibodies raised against the enzyme from higher plants.

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Selected References

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