Abstract
Cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species play an important role in the interaction of these bacteria with plant hosts. In this study, we show that (i) the neutral cyclic glucans are the biosynthetic precursors of anionic cyclic glucans; (ii) the conversion of neutral to anionic glucans is much more rapid and more extensive in exponentially growing cultures than in cultures in the stationary phase, although the latter synthesize large amounts of glucan; and (iii) the excretion of glucan, as well as the total amount synthesized, is strongly influenced by the medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beringer J. E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974 Sep;84(1):188–198. doi: 10.1099/00221287-84-1-188. [DOI] [PubMed] [Google Scholar]
- Gray G. R. Antibodies to carbohydrates: preparation of antigens by coupling carbohydrates to proteins by reductive amination with cyanoborohydride. Methods Enzymol. 1978;50:155–160. doi: 10.1016/0076-6879(78)50014-1. [DOI] [PubMed] [Google Scholar]
- Jackson B. J., Kennedy E. P. The biosynthesis of membrane-derived oligosaccharides. A membrane-bound phosphoglycerol transferase. J Biol Chem. 1983 Feb 25;258(4):2394–2398. [PubMed] [Google Scholar]
- Kennedy E. P., Rumley M. K., Schulman H., Van Golde L. M. Identification of sn-glycero-1-phosphate and phosphoethanolamine residues linked to the membrane-derived Oligosaccharides of Escherichia coli. J Biol Chem. 1976 Jul 25;251(14):4208–4213. [PubMed] [Google Scholar]
- Miller K. J., Gore R. S., Benesi A. J. Phosphoglycerol substituents present on the cyclic beta-1,2-glucans of Rhizobium meliloti 1021 are derived from phosphatidylglycerol. J Bacteriol. 1988 Oct;170(10):4569–4575. doi: 10.1128/jb.170.10.4569-4575.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller K. J., Kennedy E. P., Reinhold V. N. Osmotic adaptation by gram-negative bacteria: possible role for periplasmic oligosaccharides. Science. 1986 Jan 3;231(4733):48–51. doi: 10.1126/science.3941890. [DOI] [PubMed] [Google Scholar]
- Miller K. J., Reinhold V. N., Weissborn A. C., Kennedy E. P. Cyclic glucans produced by Agrobacterium tumefaciens are substituted with sn-1-phosphoglycerol residues. Biochim Biophys Acta. 1987 Jul 10;901(1):112–118. doi: 10.1016/0005-2736(87)90262-8. [DOI] [PubMed] [Google Scholar]
- Stanfield S. W., Ielpi L., O'Brochta D., Helinski D. R., Ditta G. S. The ndvA gene product of Rhizobium meliloti is required for beta-(1----2)glucan production and has homology to the ATP-binding export protein HlyB. J Bacteriol. 1988 Aug;170(8):3523–3530. doi: 10.1128/jb.170.8.3523-3530.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zevenhuizen L. P., Scholten-Koerselman H. J. Surface carbohydrates of Rhizobium. I. Beta-1, 2-glucans. Antonie Van Leeuwenhoek. 1979;45(2):165–175. doi: 10.1007/BF00418581. [DOI] [PubMed] [Google Scholar]