Figure 6.
Quantitation of the copy number of αSCSB-NEO construct copy number per cell. DNA from T. vaginalis clones 1 and 2, transformed with the αSCSB-NEO construct and selected using 50 μg/ml paromomycin was slotted onto nitrocellulose and probed with a 32P-labeled neo gene fragment. The amount of DNA equivalent to 1–35 copies of the αSCSB-NEO construct per cell when compared with 1 μg total T. vaginalis DNA was slotted in the left two lanes. T. vaginalis DNA (1 and 0.5 μg of total) from clones 1 and 2 was slotted in the far-right lane. Copy number was calculated by counting the radioactivity in each slot using a liquid scintillation counter.