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. 1991 May;173(10):3149–3158. doi: 10.1128/jb.173.10.3149-3158.1991

Coralline shape of the bacterial nucleoid after cryofixation.

B Bohrmann 1, W Villiger 1, R Johansen 1, E Kellenberger 1
PMCID: PMC207909  PMID: 2022615

Abstract

A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs.

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Selected References

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