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. 1991 May;173(10):3238–3242. doi: 10.1128/jb.173.10.3238-3242.1991

Characterization and generation of Escherichia coli adenylate cyclase deletion mutants.

S Shah 1, A Peterkofsky 1
PMCID: PMC207922  PMID: 2022622

Abstract

Escherichia coli delta cya-283 is a 75-bp in-frame deletion overlapping the 5' end of delta cya-854; delta cya-201 is a 41-bp frameshift deletion overlapping the 3' end of delta cya-854. Sequence repeats were found at the boundaries of delta cya-283 and delta cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene.

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Selected References

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