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. 1991 Jun;173(11):3559–3563. doi: 10.1128/jb.173.11.3559-3563.1991

Chloramphenicol resistance mutations in the single 23S rRNA gene of the archaeon Halobacterium halobium.

A S Mankin 1, R A Garrett 1
PMCID: PMC207972  PMID: 2045374

Abstract

A broad range of antibiotics affecting protein biosynthesis were screened for their ability to inhibit growth of the archaeon Halobacterium halobium. Only a few drugs, including chloramphenicol, produced inhibitory effects. Mutants which showed increased resistance to chloramphenicol were isolated; of the nine tested, eight exhibited a C----U transition at position 2471 and the ninth had an A----C transversion at position 2088 of 23S rRNA. A double mutant containing both C----U (position 2471) and A----C (position 2088) mutations was isolated, but the level of its chloramphenicol resistance did not exceed that of either single-point mutant. Inferences are made concerning the functional significance of the conserved nucleotides in rRNAs.

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Selected References

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