Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Jun;173(11):3605–3608. doi: 10.1128/jb.173.11.3605-3608.1991

Further characterizations of bleomycin-sensitive (blm) mutants of Saccharomyces cerevisiae with implications for a radiomimetic model.

C W Moore 1
PMCID: PMC207981  PMID: 1710619

Abstract

Direct selection for 12 mutations (blm) conferring hypersensitivities to lethal effects of bleomycins in Saccharomyces cerevisiae resulted in mutants exhibiting cross-hypersensitivity to ionizing radiation and hydrogen peroxide. Remaining mutations did not confer cross-hypersensitivity to radiation. All blm mutations were recessive, except codominant blm3-1, and were assigned to seven complementation groups.

Full text

PDF
3605

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burger R. M., Peisach J., Horwitz S. B. Activated bleomycin. A transient complex of drug, iron, and oxygen that degrades DNA. J Biol Chem. 1981 Nov 25;256(22):11636–11644. [PubMed] [Google Scholar]
  2. D'Andrea A. D., Haseltine W. A. Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3608–3612. doi: 10.1073/pnas.75.8.3608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Demple B., Johnson A., Fung D. Exonuclease III and endonuclease IV remove 3' blocks from DNA synthesis primers in H2O2-damaged Escherichia coli. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7731–7735. doi: 10.1073/pnas.83.20.7731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DiGiuseppe J. A., Dresler S. L. Bleomycin-induced DNA repair synthesis in permeable human fibroblasts: mediation of long-patch and short-patch repair by distinct DNA polymerases. Biochemistry. 1989 Nov 28;28(24):9515–9520. doi: 10.1021/bi00450a040. [DOI] [PubMed] [Google Scholar]
  5. DiGiuseppe J. A., Hunting D. J., Dresler S. L. Aphidicolin-sensitive DNA repair synthesis in human fibroblasts damaged with bleomycin is distinct from UV-induced repair. Carcinogenesis. 1990 Jun;11(6):1021–1026. doi: 10.1093/carcin/11.6.1021. [DOI] [PubMed] [Google Scholar]
  6. Floyd R. A. DNA-ferrous iron catalyzed hydroxyl free radical formation from hydrogen peroxide. Biochem Biophys Res Commun. 1981 Apr 30;99(4):1209–1215. doi: 10.1016/0006-291x(81)90748-8. [DOI] [PubMed] [Google Scholar]
  7. Giloni L., Takeshita M., Johnson F., Iden C., Grollman A. P. Bleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage. J Biol Chem. 1981 Aug 25;256(16):8608–8615. [PubMed] [Google Scholar]
  8. Grollman A. P., Takeshita M. Interactions of bleomycin with DNA. Adv Enzyme Regul. 1980;18:67–83. doi: 10.1016/0065-2571(80)90009-6. [DOI] [PubMed] [Google Scholar]
  9. Henner W. D., Grunberg S. M., Haseltine W. A. Enzyme action at 3' termini of ionizing radiation-induced DNA strand breaks. J Biol Chem. 1983 Dec 25;258(24):15198–15205. [PubMed] [Google Scholar]
  10. Henner W. D., Rodriguez L. O., Hecht S. M., Haseltine W. A. gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini. J Biol Chem. 1983 Jan 25;258(2):711–713. [PubMed] [Google Scholar]
  11. Johnson A. W., Demple B. Yeast DNA 3'-repair diesterase is the major cellular apurinic/apyrimidinic endonuclease: substrate specificity and kinetics. J Biol Chem. 1988 Dec 5;263(34):18017–18022. [PubMed] [Google Scholar]
  12. Johnson A. W., Demple B. Yeast DNA diesterase for 3'-fragments of deoxyribose: purification and physical properties of a repair enzyme for oxidative DNA damage. J Biol Chem. 1988 Dec 5;263(34):18009–18016. [PubMed] [Google Scholar]
  13. Keller T. J., Oppenheimer N. J. Enhanced bleomycin-mediated damage of DNA opposite charged nicks. A model for bleomycin-directed double strand scission of DNA. J Biol Chem. 1987 Nov 5;262(31):15144–15150. [PubMed] [Google Scholar]
  14. Meneghini R., Hoffmann M. E. The damaging action of hydrogen peroxide on DNA of human fibroblasts is mediated by a non-dialyzable compound. Biochim Biophys Acta. 1980 Jun 27;608(1):167–173. doi: 10.1016/0005-2787(80)90144-6. [DOI] [PubMed] [Google Scholar]
  15. Moore C. W. Control of in vivo (cellular) phleomycin sensitivity by nuclear genotype, growth phase, and metal ions. Cancer Res. 1982 Mar;42(3):929–933. [PubMed] [Google Scholar]
  16. Moore C. W. Isolation and partial characterization of mutants of Saccharomyces cerevisiae altered in sensitivities to lethal effects of bleomycins. J Antibiot (Tokyo) 1980 Nov;33(11):1369–1375. doi: 10.7164/antibiotics.33.1369. [DOI] [PubMed] [Google Scholar]
  17. Moore C. W. Ligase-deficient yeast cells exhibit defective DNA rejoining and enhanced gamma ray sensitivity. J Bacteriol. 1982 Jun;150(3):1227–1233. doi: 10.1128/jb.150.3.1227-1233.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moore C. W. Responses of radiation-sensitive mutants of Saccharomyces cerevisiae to lethal effects of bleomycin. Mutat Res. 1978 Aug;51(2):165–180. doi: 10.1016/s0027-5107(78)80016-5. [DOI] [PubMed] [Google Scholar]
  19. Moore C. W., Schmick A. Genetic effects of impure and pure saccharin in yeast. Science. 1979 Sep 7;205(4410):1007–1010. doi: 10.1126/science.382356. [DOI] [PubMed] [Google Scholar]
  20. Moore C. W., Sherman F. Role of DNA sequences in genetic recombination in the iso-1-cytochrome c gene of yeast. I. Discrepancies between physical distances and genetic distances determined by five mapping procedures. Genetics. 1975 Mar;79(3):397–418. doi: 10.1093/genetics/79.3.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Moore C. W. cdc9 ligase-defective mutants of Saccharomyces cerevisiae exhibit lowered resistance to lethal effects of bleomycin. J Bacteriol. 1982 Sep;151(3):1617–1620. doi: 10.1128/jb.151.3.1617-1620.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Murugesan N., Xu C., Ehrenfeld G. M., Sugiyama H., Kilkuskie R. E., Rodriguez L. O., Chang L. H., Hecht S. M. Analysis of products formed during bleomycin-mediated DNA degradation. Biochemistry. 1985 Oct 8;24(21):5735–5744. doi: 10.1021/bi00342a008. [DOI] [PubMed] [Google Scholar]
  23. Regan J. D., Setlow R. B. Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens. Cancer Res. 1974 Dec;34(12):3318–3325. [PubMed] [Google Scholar]
  24. Shiloh Y., Tabor E., Becker Y. Abnormal response of ataxia-telangiectasia cells to agents that break the deoxyribose moiety of DNA via a targeted free radical mechanism. Carcinogenesis. 1983 Oct;4(10):1317–1322. doi: 10.1093/carcin/4.10.1317. [DOI] [PubMed] [Google Scholar]
  25. Takeshita M., Grollman A. P., Ohtsubo E., Ohtsubo H. Interaction of bleomycin with DNA. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5983–5987. doi: 10.1073/pnas.75.12.5983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES