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. 1991 Jun;173(12):3770–3775. doi: 10.1128/jb.173.12.3770-3775.1991

Properties of the streptomycete temperate bacteriophage FP43.

D R Hahn 1, M A McHenney 1, R H Baltz 1
PMCID: PMC208007  PMID: 2050633

Abstract

FP43 is a temperate bacteriophage for Streptomyces griseofuscus that forms plaques on many Streptomyces species. FP43 virions contain 56 kb of double-strand DNA that is circularly permuted and terminally redundant, and contains 65% G + C. A physical map of the FP43 genome was constructed, and the origin for headful packaging (pac) was localized to an 8.8-kb region of the genome (hft) that mediates high-frequency transduction by FP43 of plasmid pRHB101. The phage attachment site (attP), a replication origin (rep), a region that inhibits plaque formation (pin), and a 3-kb deletion (rpt) that caused a 100-fold reduction in plasmid transduction were mapped.

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Selected References

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  1. Ackermann H. W., Eisenstark A. The present state of phage taxonomy. Intervirology. 1974;3(4):201–219. doi: 10.1159/000149758. [DOI] [PubMed] [Google Scholar]
  2. Anné J., Wohlleben W., Burkardt H. J., Springer R., Pühler A. Morphological and molecular characterization of several actinophages isolated from soil which lyse Streptomyces cattleya or S. venezuelae. J Gen Microbiol. 1984 Oct;130(10):2639–2649. doi: 10.1099/00221287-130-10-2639. [DOI] [PubMed] [Google Scholar]
  3. Baltz R. H. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. J Gen Microbiol. 1978 Jul;107(1):93–102. doi: 10.1099/00221287-107-1-93. [DOI] [PubMed] [Google Scholar]
  4. Birch A. W., Cullum J. Temperature-sensitive mutants of the Streptomyces plasmid pIJ702. J Gen Microbiol. 1985 Jun;131(6):1299–1303. doi: 10.1099/00221287-131-6-1299. [DOI] [PubMed] [Google Scholar]
  5. Cox K. L., Baltz R. H. Restriction of bacteriophage plaque formation in Streptomyces spp. J Bacteriol. 1984 Aug;159(2):499–504. doi: 10.1128/jb.159.2.499-504.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dowding J. E. Characterization of a bacteriophage virulent for Streptomyces coelicolor A3(2). J Gen Microbiol. 1973 May;76(1):163–176. doi: 10.1099/00221287-76-1-163. [DOI] [PubMed] [Google Scholar]
  7. Fishman S. E., Hershberger C. L. Amplified DNA in Streptomyces fradiae. J Bacteriol. 1983 Aug;155(2):459–466. doi: 10.1128/jb.155.2.459-466.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hahn D. R., McHenney M. A., Baltz R. H. Characterization of FP22, a large streptomycete bacteriophage with DNA insensitive to cleavage by many restriction enzymes. J Gen Microbiol. 1990 Dec;136(12):2395–2404. doi: 10.1099/00221287-136-12-2395. [DOI] [PubMed] [Google Scholar]
  9. Jackson E. N., Jackson D. A., Deans R. J. EcoRI analysis of bacteriophage P22 DNA packaging. J Mol Biol. 1978 Jan 25;118(3):365–388. doi: 10.1016/0022-2836(78)90234-6. [DOI] [PubMed] [Google Scholar]
  10. Katz E., Thompson C. J., Hopwood D. A. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol. 1983 Sep;129(9):2703–2714. doi: 10.1099/00221287-129-9-2703. [DOI] [PubMed] [Google Scholar]
  11. Kuhstoss S., Richardson M. A., Rao R. N. Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2. J Bacteriol. 1989 Jan;171(1):16–23. doi: 10.1128/jb.171.1.16-23.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  13. Matsushima P., Baltz R. H. Genetic transformation of Micromonospora rosaria by the Streptomyces plasmid pIJ702. J Antibiot (Tokyo) 1988 Apr;41(4):583–585. doi: 10.7164/antibiotics.41.583. [DOI] [PubMed] [Google Scholar]
  14. Matsushima P., Baltz R. H. Streptomyces lipmanii expresses two restriction systems that inhibit plasmid transformation and bacteriophage plaque formation. J Bacteriol. 1989 Jun;171(6):3128–3132. doi: 10.1128/jb.171.6.3128-3132.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Matsushima P., Cox K. L., Baltz R. H. Highly transformable mutants of Streptomyces fradiae defective in several restriction systems. Mol Gen Genet. 1987 Mar;206(3):393–400. doi: 10.1007/BF00428877. [DOI] [PubMed] [Google Scholar]
  16. Matsushima P., McHenney M. A., Baltz R. H. Efficient transformation of Amycolatopsis orientalis (Nocardia orientalis) protoplasts by Streptomyces plasmids. J Bacteriol. 1987 May;169(5):2298–2300. doi: 10.1128/jb.169.5.2298-2300.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Matsushima P., McHenney M. A., Baltz R. H. Transduction and transformation of plasmid DNA in Streptomyces fradiae strains that express different levels of restriction. J Bacteriol. 1989 Jun;171(6):3080–3084. doi: 10.1128/jb.171.6.3080-3084.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McHenney M. A., Baltz R. H. Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43. J Bacteriol. 1988 May;170(5):2276–2282. doi: 10.1128/jb.170.5.2276-2282.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McHenney M. A., Baltz R. H. Transduction of plasmid DNA in macrolide producing streptomycetes. J Antibiot (Tokyo) 1989 Nov;42(11):1725–1727. doi: 10.7164/antibiotics.42.1725. [DOI] [PubMed] [Google Scholar]
  20. Roberts R. J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2331–2365. doi: 10.1093/nar/18.suppl.2331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Solenberg P. J., Baltz R. H. Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus. J Bacteriol. 1991 Feb;173(3):1096–1104. doi: 10.1128/jb.173.3.1096-1104.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Susskind M. M., Botstein D. Molecular genetics of bacteriophage P22. Microbiol Rev. 1978 Jun;42(2):385–413. doi: 10.1128/mr.42.2.385-413.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Youderian P., Sugiono P., Brewer K. L., Higgins N. P., Elliott T. Packaging specific segments of the Salmonella chromosome with locked-in Mud-P22 prophages. Genetics. 1988 Apr;118(4):581–592. doi: 10.1093/genetics/118.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]

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