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. 1991 Jun;173(12):3914–3917. doi: 10.1128/jb.173.12.3914-3917.1991

Bacterial DNA supercoiling and [ATP]/[ADP] ratio: changes associated with salt shock.

L S Hsieh 1, J Rouviere-Yaniv 1, K Drlica 1
PMCID: PMC208027  PMID: 1646791

Abstract

When Escherichia coli K-12 was shifted from a medium lacking salt to one containing 0.5 M NaCl, both the [ATP]/[ADP] ratio and negative supercoiling of plasmid DNA increased within a few minutes. After about 10 min both declined, eventually reaching a level slightly above that observed with cells growing exponentially in the absence of salt. Since in vitro the [ATP]/[ADP] ratio influences the level of supercoiling generated by gyrase (H. Westerhoff, M. O'Dea, A. Maxwell, and M. Gellert, Cell Biophys. 12:157-181, 1988), the physiological response of supercoiling to salt shock is most easily explained by the sensitivity of gyrase to changes in the intracellular [ATP]/[ADP] ratio. This raises the possibility that the [ATP]/[ADP] ratio is an important factor in the control of supercoiling.

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Selected References

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