Abstract
The presence of reverse gyrase, an unusual ATP-dependent type I topoisomerase first isolated from thermophilic archaebacteria, has been detected in four strains of Thermotogales, an order of extremely thermophilic eubacteria. This result suggests that reverse gyrase plays a key role in high-temperature-living organisms, independently of the evolutionary kingdom to which they belong.
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Selected References
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- Bouthier de la Tour C., Portemer C., Nadal M., Stetter K. O., Forterre P., Duguet M. Reverse gyrase, a hallmark of the hyperthermophilic archaebacteria. J Bacteriol. 1990 Dec;172(12):6803–6808. doi: 10.1128/jb.172.12.6803-6808.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forterre P., Mirambeau G., Jaxel C., Nadal M., Duguet M. High positive supercoiling in vitro catalyzed by an ATP and polyethylene glycol-stimulated topoisomerase from Sulfolobus acidocaldarius. EMBO J. 1985 Aug;4(8):2123–2128. doi: 10.1002/j.1460-2075.1985.tb03902.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kikuchi A., Asai K. Reverse gyrase--a topoisomerase which introduces positive superhelical turns into DNA. Nature. 1984 Jun 21;309(5970):677–681. doi: 10.1038/309677a0. [DOI] [PubMed] [Google Scholar]
- Mead D. A., Szczesna-Skorupa E., Kemper B. Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng. 1986 Oct-Nov;1(1):67–74. doi: 10.1093/protein/1.1.67. [DOI] [PubMed] [Google Scholar]
- Nadal M., Jaxel C., Portemer C., Forterre P., Mirambeau G., Duguet M. Reverse gyrase of Sulfolobus: purification to homogeneity and characterization. Biochemistry. 1988 Dec 27;27(26):9102–9108. doi: 10.1021/bi00426a006. [DOI] [PubMed] [Google Scholar]
- Nakasu S., Kikuchi A. Reverse gyrase; ATP-dependent type I topoisomerase from Sulfolobus. EMBO J. 1985 Oct;4(10):2705–2710. doi: 10.1002/j.1460-2075.1985.tb03990.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
- Williams M. R., Gilson D., Marsh L., Morgan D. A., Nicholson R. I., Elston C. W., Griffiths K., Blamey R. W. The early results from a randomised study of radiotherapy versus Nolvadex (tamoxifen) as initial treatment for stage III breast cancer. Eur J Surg Oncol. 1988 Jun;14(3):235–240. [PubMed] [Google Scholar]
- Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]