Abstract
The presence of reverse gyrase, an unusual ATP-dependent type I topoisomerase first isolated from thermophilic archaebacteria, has been detected in four strains of Thermotogales, an order of extremely thermophilic eubacteria. This result suggests that reverse gyrase plays a key role in high-temperature-living organisms, independently of the evolutionary kingdom to which they belong.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bouthier de la Tour C., Portemer C., Nadal M., Stetter K. O., Forterre P., Duguet M. Reverse gyrase, a hallmark of the hyperthermophilic archaebacteria. J Bacteriol. 1990 Dec;172(12):6803–6808. doi: 10.1128/jb.172.12.6803-6808.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forterre P., Mirambeau G., Jaxel C., Nadal M., Duguet M. High positive supercoiling in vitro catalyzed by an ATP and polyethylene glycol-stimulated topoisomerase from Sulfolobus acidocaldarius. EMBO J. 1985 Aug;4(8):2123–2128. doi: 10.1002/j.1460-2075.1985.tb03902.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kikuchi A., Asai K. Reverse gyrase--a topoisomerase which introduces positive superhelical turns into DNA. Nature. 1984 Jun 21;309(5970):677–681. doi: 10.1038/309677a0. [DOI] [PubMed] [Google Scholar]
- Mead D. A., Szczesna-Skorupa E., Kemper B. Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng. 1986 Oct-Nov;1(1):67–74. doi: 10.1093/protein/1.1.67. [DOI] [PubMed] [Google Scholar]
- Nadal M., Jaxel C., Portemer C., Forterre P., Mirambeau G., Duguet M. Reverse gyrase of Sulfolobus: purification to homogeneity and characterization. Biochemistry. 1988 Dec 27;27(26):9102–9108. doi: 10.1021/bi00426a006. [DOI] [PubMed] [Google Scholar]
- Nakasu S., Kikuchi A. Reverse gyrase; ATP-dependent type I topoisomerase from Sulfolobus. EMBO J. 1985 Oct;4(10):2705–2710. doi: 10.1002/j.1460-2075.1985.tb03990.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
- Williams M. R., Gilson D., Marsh L., Morgan D. A., Nicholson R. I., Elston C. W., Griffiths K., Blamey R. W. The early results from a randomised study of radiotherapy versus Nolvadex (tamoxifen) as initial treatment for stage III breast cancer. Eur J Surg Oncol. 1988 Jun;14(3):235–240. [PubMed] [Google Scholar]
- Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]