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. 1991 Aug;173(15):4904–4907. doi: 10.1128/jb.173.15.4904-4907.1991

Role of translation of the pheA leader peptide coding region in attenuation regulation of the Escherichia coli pheA gene.

N Gavini 1, L Pulakat 1
PMCID: PMC208172  PMID: 1856183

Abstract

In Escherichia coli, the expression of the pheA gene is regulated by attenuation of transcription. To study the molecular details of pheA attenuation, we introduced mutations in the pheA leader peptide coding region and analyzed their effects by using pheA promoter-lacZ gene transcription fusions (pheAp-lacZ). Mutations in the ribosome-binding site (pheAe1213) or in the translation initiation codon (pheAe24) of the pheA leader peptide coding region resulted in superattenuation of pheA expression. However, the presence of a stop codon upstream to the tandem phenylalanine codons (pheAe3334) led to an increase in the basal-level expression of pheA. This increase was further enhanced in the presence of prfA release factor mutant. The level of pheA expression in all three mutants was the same when cells were starved for phenylalanine. These results demonstrate that efficient translation of the pheA leader peptide coding region and the position of the ribosome on the leader transcript play decisive roles in the attenuation regulation of pheA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen J. W., Bennett D. C., Umbarger H. E. Specificity of attenuation control in the ilvGMEDA operon of Escherichia coli K-12. J Bacteriol. 1991 Apr;173(7):2328–2340. doi: 10.1128/jb.173.7.2328-2340.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gavini N., Davidson B. E. Regulation of pheA expression by the pheR product in Escherichia coli is mediated through attenuation of transcription. J Biol Chem. 1991 Apr 25;266(12):7750–7753. [PubMed] [Google Scholar]
  3. Gavini N., Davidson B. E. The pheR gene of Escherichia coli encodes tRNA(Phe), not a repressor protein. J Biol Chem. 1990 Dec 15;265(35):21527–21531. [PubMed] [Google Scholar]
  4. Gavini N., Davidson B. E. pheAo mutants of Escherichia coli have a defective pheA attenuator. J Biol Chem. 1990 Dec 15;265(35):21532–21535. [PubMed] [Google Scholar]
  5. Gavini N., Pulakat L. Role of ribosome release in the basal level of expression of the Escherichia coli gene pheA. J Gen Microbiol. 1991 Mar;137(3):679–684. doi: 10.1099/00221287-137-3-679. [DOI] [PubMed] [Google Scholar]
  6. Gowrishankar J., Pittard J. Regulation of phenylalanine biosynthesis in Escherichia coli K-12: control of transcription of the pheA operon. J Bacteriol. 1982 Jun;150(3):1130–1137. doi: 10.1128/jb.150.3.1130-1137.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Im S. W., Pittard J. Phenylalanine biosynthesis in Escherichia coli K-12: mutants derepressed for chorismate mutase P-prephenate dehydratase. J Bacteriol. 1971 Jun;106(3):784–790. doi: 10.1128/jb.106.3.784-790.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kieny M. P., Lathe R., Lecocq J. P. New versatile cloning and sequencing vectors based on bacteriophage M13. Gene. 1983 Dec;26(1):91–99. doi: 10.1016/0378-1119(83)90039-2. [DOI] [PubMed] [Google Scholar]
  9. Landick R., Yanofsky C., Choo K., Phung L. Replacement of the Escherichia coli trp operon attenuation control codons alters operon expression. J Mol Biol. 1990 Nov 5;216(1):25–37. doi: 10.1016/S0022-2836(05)80058-0. [DOI] [PubMed] [Google Scholar]
  10. Miller J. H., Albertini A. M. Effects of surrounding sequence on the suppression of nonsense codons. J Mol Biol. 1983 Feb 15;164(1):59–71. doi: 10.1016/0022-2836(83)90087-6. [DOI] [PubMed] [Google Scholar]
  11. Roesser J. R., Nakamura Y., Yanofsky C. Regulation of basal level expression of the tryptophan operon of Escherichia coli. J Biol Chem. 1989 Jul 25;264(21):12284–12288. [PubMed] [Google Scholar]
  12. Roesser J. R., Yanofsky C. Ribosome release modulates basal level expression of the trp operon of Escherichia coli. J Biol Chem. 1988 Oct 5;263(28):14251–14255. [PubMed] [Google Scholar]
  13. Ryden M., Murphy J., Martin R., Isaksson L., Gallant J. Mapping and complementation studies of the gene for release factor 1. J Bacteriol. 1986 Dec;168(3):1066–1069. doi: 10.1128/jb.168.3.1066-1069.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rydén S. M., Isaksson L. A. A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors. Mol Gen Genet. 1984;193(1):38–45. doi: 10.1007/BF00327411. [DOI] [PubMed] [Google Scholar]
  15. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  16. Simons R. W., Houman F., Kleckner N. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene. 1987;53(1):85–96. doi: 10.1016/0378-1119(87)90095-3. [DOI] [PubMed] [Google Scholar]
  17. Yanofsky C., Kelley R. L., Horn V. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe. J Bacteriol. 1984 Jun;158(3):1018–1024. doi: 10.1128/jb.158.3.1018-1024.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yanofsky C. Transcription attenuation. J Biol Chem. 1988 Jan 15;263(2):609–612. [PubMed] [Google Scholar]
  19. Zurawski G., Brown K., Killingly D., Yanofsky C. Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4271–4275. doi: 10.1073/pnas.75.9.4271. [DOI] [PMC free article] [PubMed] [Google Scholar]

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