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. 1991 Sep;173(17):5568–5572. doi: 10.1128/jb.173.17.5568-5572.1991

A transducing bacteriophage for Caulobacter crescentus uses the paracrystalline surface layer protein as a receptor.

P Edwards 1, J Smit 1
PMCID: PMC208274  PMID: 1885534

Abstract

The bacteriophage phi Cr30, a transducing phage for Caulobacter crescentus strains, required the paracrystalline surface (S) layer for infectivity. Wild-type strains were phage resistant when rsaA, the gene for the 130K S-layer protein, was interrupted with an antibiotic resistance cassette. Strains that had lost the S layer by mutation were phage resistant, as were mutants that produce an S layer but which do not attach the structure to the cell surface. Phage sensitivity was restored to 130K-protein-deficient strains by introducing rsaA on a plasmid. Spontaneous phage-resistant strains produced expected phenotypes as follows (in order of decreasing frequency): S-layer cell attachment defects, no S layer, or an S layer that was wild type in appearance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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