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. 1991 Sep;173(17):5573–5577. doi: 10.1128/jb.173.17.5573-5577.1991

Tn5099, a xylE promoter probe transposon for Streptomyces spp.

D R Hahn 1, P J Solenberg 1, R H Baltz 1
PMCID: PMC208275  PMID: 1653213

Abstract

Tn5099, a promoter probe transposon for Streptomyces spp., was constructed by inserting a promoterless xylE gene and a hygromycin resistance gene into IS493. Tn5099 transposed into different sites in the Streptomyces griseofuscus genome, and the xylE reporter gene was expressed in some of the transposition mutants. Strains containing Tn5099 insertions that gave regulated expression of the xylE gene were identified.

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Selected References

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