Abstract
Background
Thyroid imaging is helpful in confirming the diagnosis of congenital hypothyroidism and in establishing the aetiology. Although isotope scanning is the standard method of imaging, ultrasound assessment may be complementary.
Aim
To determine the strengths and weaknesses of thyroid ultrasound and isotope scanning in neonates with thyroid stimulating hormone (TSH) elevation.
Methods
Babies from the West of Scotland with raised capillary TSH (>15 mU/l) on neonatal screening between January 1999 and 2004 were recruited. Thyroid dimensions were measured using ultrasonography, and volumes were calculated. Isotope scanning was carried out with a pinhole collimator after an intravenous injection of 99m‐technetium pertechnetate.
Results
40 infants (29 female) underwent scanning at a median of 17 days (range 12 days to 15 months). The final diagnosis was athyreosis (n = 11), ectopia (n = 12), hypoplasia (n = 8; 3 cases of hemi‐agenesis), dyshormonogenesis (n = 5), transient hypothyroidism (n = 2), transient hyperthyrotropinaemia (n = 1) and uncertain status with gland in situ (n = 1). 6 infants had discordant scans with no isotope uptake but visualisation of thyroid tissue on ultrasound. This was attributed to TSH suppression from thyroxine (n = 3); maternal blocking antibodies (n = 1); cystic degeneration of the thyroid (n = 1); and possible TSH receptor defect (n = 1).
Conclusions
Isotope scanning was superior to ultrasound in the detection of ectopic tissue. However, ultrasound detected tissue that was not visualised on isotope scanning, and showed abnormalities of thyroid volume and morphology. We would therefore advocate dual scanning in newborns with TSH elevation as each modality provides different information.
Congenital hypothyroidism is a relatively common congenital disorder with an incidence of 1 in 4350 live births in Scotland.1 It is one of the few preventable causes of mental retardation. Congenital hypothyroidism is usually seen in otherwise healthy term neonates who are found to have a significant increase in the thyroid stimulating hormone (TSH>50 mU/l) on Guthrie screening carried out between 5 and 7 days of age.1 Approximately 80% of congenital hypothyroidism is caused by thyroid dysgenesis due to absence, hypoplasia or ectopia of the gland, which is almost always sporadic in nature. The other 15–20% of cases are caused by a variety of autosomal recessive defects affecting thyroxine (T4) synthesis (dyshormonogenesis). In such cases the gland is nearly always normal or enlarged.
Transient thyroid dysfunction (TTD) is usually seen in association with prematurity, sickness and congenital malformation.2 Congenital hypothyroidism and TTD with increased TSH level cannot always be distinguished on clinical grounds, and current practice is to treat all but the mildest cases with thyroxine and re‐evaluate thyroid status after 2 or 3 years.3 Re‐evaluation constitutes either phasing out T4 treatment or converting to T3 treatment and then stopping for 2 weeks, followed by biochemical evaluation and isotope imaging. We have found this problematic; it is time consuming, technically difficult owing to poor cooperation in young children, and may render the child symptomatic from hypothyroidism.
With true congenital hypothyroidism, there has always been a case for carrying out thyroid imaging to determine the aetiology, as 20% of cases will be due to dyshormonogenesis, which carries a 1 in 4 recurrence risk. Neonatal screening was started in Scotland in 19794 and isotope scanning was regularly carried out until 1983 when enthusiasm began to wane, possibly due to the practical difficulties.5
Since the initial description of the thyroid transcription factor Pax‐8 in 1992,6 advances have been made in the understanding of both normal thyroid development and the aetiology of congenital hypothyroidism. These include the identification of further transcription factors, TTF‐1 in 19957 and TTF‐2 in 1997,8 and the description of people with inactivating TSH receptor defects.9,10 Infants with congenital hypothyroidism due to a thyroid gland in situ show a greater diagnostic yield in terms of specific aetiology.11 Determining the thyroid site and size by imaging is therefore desirable.
Currently, isotope scanning is the gold standard in imaging babies and children with congenital hypothyroidism, and the only reliable method of disclosing an ectopic gland, although it is less helpful in the assessment of thyroid size and morphology. However, most scanning is carried out in adult units and the results may be difficult to interpret, especially in babies who have received iodine in the course of antiseptic procedures, as this interferes with the uptake of isotope.12 Moreover, it is not practical to carry out isotope scanning in sick, preterm infants.
Although ultrasound is a promising technique for thyroid imaging in newborn infants with congenital hypothyroidism,13,14 it is still relatively underutilised, especially in the UK. In 1990, De Bruyn et al15 reported that thyroid ultrasound was only of limited value in the assessment of congenital hypothyroidism. However, there has been renewed interest recently, with several publications showing the value of thyroid ultrasonography in neonates with congenital hypothyroidism.16,17,18,19 For example, additional phenotypic abnormalities in infants with thyroid dysgenesis have been shown, including cysts along the normal pathway of the thyroglossal duct and cysts or thymic tissue in the empty thyroid area.17,20 Normative data for thyroid ultrasonography in newborns have been published by two groups in Germany and Belgium,21,22 and we have determined normative ranges for thyroid volume in 100 (49 male) healthy term infants from Glasgow.23
In theory, thyroid ultrasound would not be able to identify a lingual gland, but Ueda et al24 have carried this out successfully using two basic scanning positions: the midline sagittal and posterior coronal views of the floor of the mouth. However, colour Doppler ultrasonography has recently been shown to be superior to both grey‐scale ultrasound and magnetic resonance imaging scanning for detecting ectopic thyroid tissue and may be a useful diagnostic tool.25
Our objective was to carry out thyroid ultrasound scanning on babies with increased TSH level on Guthrie card screening or venous samples and compare the findings with those on isotope scanning to assess the strengths and weaknesses of each diagnostic modality.
Methods
Patients
After receiving approval from the Yorkhill Ethics Committee (Yorkhill, Glasgow, UK), all West of Scotland paediatricians were informed of the study. The responsible paediatrician was then invited to allow individual patients to participate in the study at the time of referral by the screening laboratory. Thyroxine treatment was started without delay (unless increase in TSH level was mild), and irrespective of when the scans could be carried out (see later).
Parents were asked to bring their infant for dual scanning in the Diagnostic Imaging Department of the Royal Hospital for Sick Children, Glasgow, UK. When intravenous cannula was inserted for administration of an isotope tracer, venous blood was obtained for thyroid function tests. Scans were carried out as early as possible, ideally within 7 days of starting treatment.
Babies with increased TSH level associated with extreme prematurity, sickness or congenital malformation were excluded from the study.
Ultrasound scanning
All infants were scanned by a paediatric radiologist (either SM or ACM) using an ACUSON 128XP 10 System (Acuson, Mountain View, California, USA) or an ATL 5000 HDI system (Bothwell, WA, California, USA). Transverse and longitudinal sections of the thyroid gland were performed using a linear 7.5 MHz probe or CL15‐7 MHz probe with prewarmed coupling gel. The precise anatomical location of the thyroid was determined (transversely inferior to the thyroid cartilage and medial to the common carotid artery and jugular vein); then measurements of the maximum length of the gland from the sagittal images of both thyroid lobes were recorded. The maximum transverse diameter and the maximum depth of each lobe were measured from the transverse images. The thyroid volume was calculated using the formula for a prolate ellipsoid:
Thyroid volume = length×breadth×depth×π/6.
Thyroid volumes were compared with normative data for the newborn.23 The echogenicity of the thyroid was routinely assessed by comparison with the adjacent neck muscles as the thyroid gland is usually slightly hyperechoic, whereas fat is much more echogenic.26 The sonographers were not blinded to the isotope findings by design; however, the isotope reports were not available at the time of ultrasound scanning, as both scans were carried out on the same day.
Isotope scanning
Isotope scanning was carried out with a pinhole collimator after an intravenous injection of 10–20 MBq 99m‐technetium pertechnetate. An initial antero‐posterior scan was performed before the placement of a marker (cobalt pen) in the sternal notch. Lateral and antero‐posterior scans (including the abdomen) were then obtained. The normally sited thyroid gland lies posterior to the marker on the lateral scan. The level of radioisotope uptake by the thyroid was compared with the uptake by the salivary glands and stomach. The scans were interpreted by one of the paediatric radiologists (SM or ACM) in all but five cases.
Biochemistry
Babies were reviewed by their local paediatrician, and venous blood for total T4/free T4 (fT4) and TSH was obtained to confirm the diagnosis. Infants were started on thyroxine treatment at a median or mean (range) dose of 50 or 40.4 (12.5–50) μg/day.
On the day of scanning, venous blood was drawn for TSH and fT4. These were measured using the DPC Immulite 2000 (Diagnostic Products Corporation, California, USA).
The diagnosis of congenital hypothyroidism was made for each patient using all the information available (initial biochemistry, TSH at the time of isotope scan or duration of thyroxine treatment, family history and dual scanning results). Two clinicians (RJP and MDCD) reviewed the clinical, biochemical and radiological data in each case and agreed on a final aetiological diagnosis of congenital hypothyroidism.
Statistical considerations
In view of the small numbers, no formal statistics were applied, and a descriptive analysis was carried out.
Results
Patient, maternal and screening data
During the study period, 76 infants (53 female) had an increased TSH level on Guthrie card screening. Table 1 summarises the clinical details of the infants (n = 40) included in the study (29 female). Of the remaining 36 infants, 15 had a single scan performed (ultrasound or isotope) and 21 had no imaging. The final diagnosis of the single scanned group is as follows: definite congenital hypothyroidism in 6, transient increased TSH level in 4 and uncertain status in 5 (4 with Down's syndrome). The final diagnosis of the unscanned group: definite congenital diagnosis in 6, transient increased TSH level in 9 and status uncertain in 6 (3 with Down's syndrome).
Table 1 Summary of all infants with increased thyroid stimulating hormone level (>15 mU/l) who underwent dual scanning.
| Sex | Guthrie TSH (days) | Ven TSH (days) | Ven T4 (days) | Age at Rx (days) | Ultrasound report | Isotope scanning | Diagnosis | Conc | |
|---|---|---|---|---|---|---|---|---|---|
| Report; TSH/age (days) | Day of Rx | ||||||||
| F | 173 (7) | 477 (13) | 7 (13) | 13 | No tissue | Faint uptake base of tongue; 5.8/27 | 15 | E | Yes |
| F | 152 (8) | 155.3 (16) | 10 (16) | 15 | No tissue | Small area base of tongue; NA/17 | 2 | E | Yes |
| M | 83 (5) | NA (11) | 79* (11) | 11 | Lingual thyroid | Uptake thyroid region; 76.8/14 | 4 | E | Yes |
| F | 465 (8) | 464 (14) | 7.2 (14) | 14 | No tissue | Sublingual thyroid; 26/20 | 7 | E | Yes |
| F | 238 (7) | 514 (11) | 5.7 (11) | 11 | No tissue | Small area midline of tongue; NA/15 | 5 | E | Yes |
| F | 135 (7) | >100 (9) | 66 (9) | 9 | No tissue | Lingual uptake in midline; 70.8/15 | 7 | E | Yes |
| M | 78 (7) | 370 (12) | 9.2 (12) | 12 | No tissue | Small area post. to tongue; 13.2/17 | 6 | E | Yes |
| F | 165 (6) | >100 (9) | 26.7 (9) | 10 | No tissue | Lingual thyroid; NA/15 | 6 | E | Yes |
| F | 42 (5) | 22.6 (12) | 15.7 (12) | 58 | No tissue | Uptake higher, more post; NA/30 | 0 | E | Yes |
| M | 149 (7) | 354.5 (13) | 6.2 (13) | 13 | No tissue | Tiny area base of tongue; 354 (13d)/14 | 2 | E | Yes |
| F | 41 (7) | 20.7 (10) | 16.3 (10) | 155 | No tissue | Small sublingual; NA/49 | 0 | E | Yes |
| F | 140 (5) | >100 (8) | 70* (8) | 8 | No tissue | Focal tracer base of tongue; NA/13 | 6 | E | Yes |
| F | 447 (6) | >150 (10) | 29* (10) | 10 | No tissue; cysts on left | No uptake; NA/17 | 8 | A | Yes |
| M | 246 (5) | 141.1 (11) | 5 (11) | 11 | No tissue | No uptake; NA/27 | 7 | A | Yes |
| M | 271 (5) | >100 (11) | 3.7 (11) | 11 | No tissue | No uptake: extravasation; NA/14 | 4 | A | Yes |
| F | 301 (5) | 660 (10) | 8 (10) | 10 | No tissue | No uptake; 59.5/15 | 6 | A | Yes |
| F | 285 (5) | >150 (9) | 4.1 (10) | 8 | No tissue | No uptake; 49.7 (35 days)/17 | 10 | A | Yes |
| F | 159 (5) | >150 (10) | <20* (10) | 10 | No tissue | No uptake; 204/16 | 7 | A | Yes |
| M | 279 (5) | 561 (12) | <6 (12) | 12 | No tissue | No uptake; 44.6/20 | 9 | A | Yes |
| F | 146 (5) | >150 (11) | 3 (11) | 11 | No tissue | No uptake; 70.4/16 | 6 | A | Yes |
| F | 258 (6) | 616.9 (12) | 3.6 (12) | 11 | No tissue | No uptake; 7.1 (14.5 months)/15 months | 15mo | A | Yes |
| F | 221 (6) | >150 (13) | <20* (13) | 13 | No tissue | No uptake; 6.7 (22 days)/16 | 4 | A | Yes |
| F | 163 (5) | >150 (9) | 35* (9) | 9 | No tissue | No uptake; 2.8/23 | 15 | A | Yes |
| F | 27 (7) | 7.4 (16) | 18 (16) | None | Left hemi‐agenesis | Slightly asymmetrical; 7.3/8 months | 0 | L Hemi | Yes |
| F | 54 (7) | 55 (12) | 17 (12) | 15 | Right hemi‐agenesis | Uptake but formless oval; NA/19 | 5 | R Hemi | Yes |
| F | 23 (9) | 16.2 (22) | 14 (22) | 207 | Rounded nodule | Rounded area of uptake; NA/5 months | 0 | Hypo | Yes |
| F | 255 (6) | >150 (8) | 84 (8) | 8 | Small | Faint uptake midline; 11.7/15 | 8 | Hypo | Yes |
| F | 208 (7) | 72.3 (10) | 4.5 (10) | 12 | Large | Good uptake; NA/17 | 6 | D | Yes |
| F | 49 (7) | >100 (14) | 8 (14) | 14 | Large | Globally enlarged; 68.3/20 | 7 | D | Yes |
| M | 16 (4) | 14.8 (36) | 9 (36) | 0 | Large | Normal; NA/2 months | 0 | D | Yes |
| F | 300 (6) | >150 (11) | <20* (11) | 11 | Large | Normal; NA/17 | 7 | D | Yes |
| M | 29 (6) | 55 (10) | 11 (10) | 10 | Normal | Normal ; 5.3/17 | 8 | D | Yes |
| M | 41 (13) | 117.8 (21) | 6.6 (21) | 21 | Normal | Normal; 10.2/28 | 8 | TH (prem) | Yes |
| F | 27 (9) | 33.6 (31) | 92* (31) | None | Small | Normal; 24.4/47 | 0 | TETSH | Yes |
| F | 55 (5) | 77 (10) | 14.1 (10) | 10 | Right hemi‐agenesis | No uptake; NA/16 | 7 | R Hemi | No |
| F | 104 (5) | >150 (10) | <5 (10) | 10 | Small | No uptake; 71.9 (14 days)/16 | 7 | Hypo | No |
| F | 120 (5) | >100 (12) | 9 (12) | 12 | Small | No uptake; 1/19 | 8 | Hypo | No |
| F | 77 (5) | 68.4 (10) | 14.3 (10) | 10 | No right lobe; cysts on left | No uptake; 7.7 (22 days)/21 | 12 | Hypo | No |
| M | 248 (5) | >150 (11) | 40 (11) | 11 | Normal size but hypoechoic | No uptake; 30.8/28 | 18 | TH(mat. abs) | No |
| M | 33 (5) | 11.87 (4) | 220* (4) | 13 | Normal | No uptake; 2.2/20 | 8 | SU | No |
A, athyreosis; conc, concordance; D, dyshormonogenesis; E, ectopia; F, female; hemi, hemi‐agenesis; hypo, hypoplasia; M, male; mat. abs, maternal blocking antibodies; NA, not applicable; prem, prematurity; Rx, start of thyroxine treatment; SU, status uncertain; T4, free thyroxine (pmol/l); TETSH, transient hyperthyrotropinaemia; TH, transient hypothyroidism; TSH, thyroid stimulating hormone; Ven, venous.
The age at neonatal (Guthrie card) screening, venepuncture and Rx is given in days. Guthrie TSH is capillary TSH on neonatal screening.
*Total thyroxine (nmol/l).
The TSH on the day of isotope scan (or nearest available day) is shown.
All but three children were Caucasian; these infants were of Pakistani, Indian and Afghani origin. Two infants (one with dyshormonogenesis and one with uncertain diagnosis with gland in situ) had affected elder siblings who did not undergo dual scanning and are not included in the study. Median (range) gestational age and birth weight was 40 (31.7–42) weeks and 3.2 (1.95–4.68) kg, respectively. Three were preterm and six were low birthweight (<2500 g) infants. Twenty five infants were of appropriate size, 13 were small and 2 were large for gestational age. Apart from one male infant with congenital dislocation of the hip, no congenital anomalies were present in this group.
In the group of infants small for gestational age two mothers had a history of substance misuse and were hepatitis C positive; one mother had epilepsy treated with lamotrigine and another had pre‐existing insulin‐dependent diabetes mellitus. In the group that was appropriate for gestational age one mother received thyroxine for autoimmune thyroid disease and another received heparin for a previous deep venous thrombosis.
For all infants the median (range) age at Guthrie screening was 6 (4–13) days; at notification 11 (8–33) days; and at start of treatment (excluding three patients who did not start thyroxine) 11 (8–207) days. These data are consistent with a recent audit of the Scottish screening programme.5 The median (range) age at dual scanning was 17 days (12 days–15 months). The median (mean) interval between starting thyroxine treatment and undergoing scanning was 7 (17.3) days, with a range of 0–446 days.
Scanning data
Tables 1 and 2 show the scan results for individual babies and aetiological groups, respectively. Thirty infants had increased TSH level >50 mU/l on Guthrie card screening and underwent dual scanning at a median age of 17 days. Ten infants with mild increase in TSH level (15–50 mU/l) were scanned later at a median age of 39 days. A variety of abnormalities were found on scanning the group with mild increase in TSH level: ectopia (n = 2), goitre (n = 3), left hemi‐agenesis (n = 1) and hypoplasia (n = 2). One infant had a thyroid gland in situ of small volume (0.63 cf 0.7–3.3 ml) on ultrasound, but with prominence of the isthmus, and a normal isotope scan. Her increase in TSH level resolved at 2 years of age and was attributed to transient isolated hyperthyrotropinaemia. The one remaining infant, born at 32 weeks, had a normal‐sized thyroid gland in situ on ultrasound and isotope scanning.
Table 2 Clinical data according to aetiological group.
| Diagnosis | No (male) | Guthrie TSH (mU/l) | Age at scans (days) | Day of thyroxine* | TSH at scans (mU/l) |
|---|---|---|---|---|---|
| Athyreosis | 11 (3) | 258 (146–447) | 16.5 (14–27)† | 7 (4–15) | 47.2 (2.8–204) |
| Ectopia | 12 (3) | 144 (41–465) | 16 (13–49) | 5.5 (0–15) | 48 (5.8–35) |
| Hypoplasia | 8 (0) | 66 (23–255) | 19 (15–243) | 7 (0–12) | 7.7 (1–71.9) |
| Dyshormono genesis | 5 (2) | 49 (16–300) | 17 (17–83) | 7 (0–8) | 37 (5–68) |
TSH, thyroid stimulating hormone.
Median (range) for each category is shown.
*The duration of thyroxine (interval between age at treatment and age at scanning) is shown in column 5.
†Except one infant who had scans at 15 months, having started treatment on day 11.
The agreed final diagnosis was athyreosis (n = 11), ectopia (n = 12), hypoplasia (n = 8), dyshormonogenesis (n = 5), transient hypothyroidism (n = 2; associated with prematurity in 1 case and maternal blocking antibodies in the other), transient hyperthyrotropinaemia (n = 1) and uncertain status with gland in situ (n = 1).
The infants tolerated the dual scanning well, with no adverse events recorded other than extravasation of radioisotope in one patient.
Isotope versus ultrasound scanning results
Table 3 compares isotope and ultrasound scan findings. Table 4 compares the duration of thyroxine treatment (ie, interval between age at treatment and age at scanning), initial dose of thyroxine and TSH at the time of scanning between groups with concordant or discordant scan results.
Table 3 Comparison of ultrasound and isotope scan findings.
| Isotope | Ultrasound | ||||||
|---|---|---|---|---|---|---|---|
| Normal | Large | Small | Absent | Ectopic | Abnormal shape | Hemi‐agenesis | |
| Normal uptake | 2 | 3 | 1 | 1 | 1 | ||
| Increased | 1 | ||||||
| Absent | 2 | 3 | 11 | 1 | |||
| Ectopic | 11 | ||||||
| Abnormal shape | 1 | 1 | |||||
| Decreased | 1 | ||||||
Bold values indicate discordant scans.
Table 4 Comparison of clinical data between groups with concordant or discordant scans.
| Scans | No (male) | Guthrie TSH (mU/l) | Age at scans (days) | Day of T4 | Initial T4 dose (mcg/day) | TSH at scan (mU/l) |
|---|---|---|---|---|---|---|
| Concordant | 34 (9) | 156 (16–465) | 17 (13–456) | 6 (0–446) | 50 (0–50) | 35.3 (5.3–354) |
| Discordant | 6 (2) | 80 (33–120) | 17.5 (16–20) | 8 (7–12) | 43.8 (25–50) | 2.2 ((1–71.9) |
T4, thyroxine; TSH, thyroid stimulating hormone.
Median (range) for each category is shown.
Discussion
Isotope scanning, as expected, proved to be superior to ultrasonography in the detection of ectopic thyroid tissue, with a sensitivity of 91.7%. We were thus unable to reproduce Ueda et al's24 100% success rate in showing the lingual thyroid on ultrasound scanning, as only one ectopic thyroid in our series was detected on ultrasonography (9.1%).
Although isotope scanning was superior in detecting ectopic thyroid tissue, ultrasound scanning detected the presence of thyroid tissue, which was not otherwise visualised in 6 (15%) of our 40 patients. The most common reason for this was the presumed suppression of TSH by thyroxine treatment. Other causes included the transfer of maternal blocking auto‐antibodies, cystic degeneration and a postulated TSH receptor defect. Ultrasound scanning can therefore help to identify those neonates with TSH receptor blocking antibody or inactivating TSH receptor defect or with an iodine‐trapping defect.
Also, we were able to show abnormalities in thyroid volume on ultrasonography that were not appreciable on isotope scanning. In cases with a normal isotope scan (n = 4), ultrasound provided evidence of goitre (n = 3) and hypoplasia (n = 1). In these cases ultrasound provided informative data to help determine aetiology. The superiority of ultrasound in determining thyroid volume has been shown previously by van Isselt et al27 in adults with Graves' disease. Ultrasound scanning was shown to be more accurate than both planar scintigraphy and single‐photon emission tomography for determining thyroid volume (using magnetic resonance imaging as the gold standard).27
Our series highlights the value of performing dual scans in infants with persistent mild increased TSH level. We found a morphological abnormality of the thyroid gland in 8 of 10 infants, in whom the increase in TSH level may otherwise have been attributed to isolated hyperthyrotropinaemia of infancy.
We have attributed non‐uptake of radioisotope to thyroxine treatment with subsequent TSH suppression in three patients, and this raises a question about the timing of scanning. It is clearly important to start treatment without delay in infants with marked increase in TSH level (>50 mU/l) but early scans are more important for those infants who have only a modest increase in TSH levels on Guthrie card screening and are likely to have lower TSH at the time of screening. We have been unable to find reports of a specific TSH cut‐off level required for adequate isotope uptake; for cancer screening a value of >30 mU/l is usually sought.28 We were able to see uptake on scans with TSH values as low as 5.3 mU/l. We would recommend taking a blood sample for TSH measurement on the day of scanning to accurately interpret the scan findings. We suggest that isotope scanning be performed within 5 days rather than 7 days of starting thyroxine treatment.
What is already known on this topic
Isotope scanning is the gold standard in imaging infants with congenital hypothyroidism.
Ultrasound scanning can show a thyroid gland in situ and may also identify ectopic tissue.
What this study adds
Ultrasound scanning can detect non‐functioning thyroid tissue not visualised with isotope scanning. It can also show abnormalities in volume and morphology, which cannot be fully appreciated on two‐dimensional isotope scans.
Dual scanning is valuable in all children with increased thyroid stimulating hormone (TSH) level, especially in those with (persistent) mild increased TSH levels, as morphological abnormalities are common.
Scans should be performed as early as possible after notification (preferably within 5 days). A blood sample for TSH measurement on the day of scanning is recommended to accurately interpret the scan results.
Although thyroglobulin could be a useful adjunct in the evaluation of infants with increased TSH levels, we did not consistently measure thyroglobulin in this group of patients.
We are currently establishing normative data in neonates and now include thyroglobulin in the routine evaluation of infants referred with increased TSH levels.
Acknowledgements
We thank the following paediatricians for allowing their patients to be studied: Christine Findlay, Ian Hunter, Lesley Jackson, Sheena Kinmond, Peter MacDonald, Helen Mactier, Mary Ray, John Schulga, Robert Simpson, Jon Staines, Graham Stewart, Tom Turner, Madeleine White and John Whyte.
Abbreviations
TSH - thyroid stimulating hormone
TTD - transient thyroid dysfunction
Footnotes
Competing interests: None.
References
- 1.Ray M, Muir T M, Murray G D.et al Audit of screening programme for congenital hypothyroidism in Scotland 1979–93. Arch Dis Child 199776411–415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Oakley G A, Muir T, Ray M.et al Increased incidence of congenital malformations in children with transient thyroid‐stimulating hormone elevation on neonatal screening. J Pediatr 1998132726–730. [DOI] [PubMed] [Google Scholar]
- 3.Donaldson M D C, Grant D B. Congenital hypothyroidism. Semin Neonatol 1998335–47. [Google Scholar]
- 4.Sutherland R M, Ratcliffe J G, Kennedy R.et al Neonatal screening for hypothyroidism in Scotland: results of a pilot study. Scott Med J 198126229–234. [DOI] [PubMed] [Google Scholar]
- 5.Jones J H, Mackenzie J, Beaton S.et al Improvement in screening performance and diagnosis of congenital hypothyroidism in Scotland 1979–2003. Arch Dis Child. Published Online First: 4 April 2006, doi:10.1136/adc.2005.088427 [DOI] [PMC free article] [PubMed]
- 6.Zannini M, Francis‐Lang H, Plachov D.et al Pax‐8, a paired domain‐containing protein, binds to a sequence overlapping the recognition site of a homeodomain and activates transcription from two thyroid‐specific promoters. Mol Cell Biol 1992124230–4241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Saiardi A, Tassi V, De Filippis V.et al Cloning and sequence analysis of human thyroid transcription factor 1. Biochim Biophys Acta 19951261307–310. [DOI] [PubMed] [Google Scholar]
- 8.Zannini M, Avantaggiato V, Biffali E.et al TTF‐2, a new forkhead protein, shows a temporal expression in the developing thyroid which is consistent with a role in controlling the onset of differentiation. EMBO J 1997163185–3197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Gagne N, Parma J, Deal C.et al Apparent congenital athyreosis contrasting with normal plasma thyroglobulin levels and associated with inactivating mutations in the thyrotropin receptor gene: are athyreosis and ectopic thyroid distinct entities? J Clin Endocrinol Metab 1998831771–1775. [DOI] [PubMed] [Google Scholar]
- 10.Abramowicz M J, Duprez L, Parma J.et al Familial congenital hypothyroidism due to inactivating mutation of the thyrotropin receptor causing profound hypoplasia of the thyroid gland. J Clin Invest 1997993018–3024. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Gruters A, Finke R, Krude H.et al Etiology grouping of permanent congenital hypothyroidism with a thyroid gland in situ. Horm Res 1994413–9. [PubMed] [Google Scholar]
- 12.l'Allemand D, Gruters A, Beyer P.et al Iodine in contrast agents and skin disinfectants is the major cause for hypothyroidism in premature infants during intensive care. Horm Res 19872842–49. [DOI] [PubMed] [Google Scholar]
- 13.Chanoine J P, Toppet V, Body J J.et al Contribution of thyroid ultrasound and serum calcitonin to the diagnosis of congenital hypothyroidism. J Endocrinol Invest 199013103–109. [DOI] [PubMed] [Google Scholar]
- 14.Dammacco F, Dammacco A, Cavallo T.et al Serum thyroglobulin and thyroid ultrasound studies in infants with congenital hypothyroidism. J Pediatr 1985106451–453. [DOI] [PubMed] [Google Scholar]
- 15.De Bruyn R, Ng W K, Taylor J.et al Neonatal hypothyroidism: comparison of radioisotope and ultrasound imaging in 54 cases. Acta Paediatr Scand 1990791194–1198. [DOI] [PubMed] [Google Scholar]
- 16.Kreisner E, Camargo‐Neto E, Maia C R.et al Accuracy of ultrasonography to establish the diagnosis and aetiology of permanent primary congenital hypothyroidism. Clin Endocrinol 200359361–365. [DOI] [PubMed] [Google Scholar]
- 17.Bubuteishvili L, Garel C, Czernichow P.et al Thyroid abnormalities by ultrasonography in neonates with congenital hypothyroidism. J Pediatr 2003143759–764. [DOI] [PubMed] [Google Scholar]
- 18.Marinovic D, Garel C, Czernichow P.et al Ultrasonographic assessment of the ectopic thyroid tissue in children with congenital hypothyroidism. PediatrRadiol200434109–113. [DOI] [PubMed] [Google Scholar]
- 19.Ohnishi H, Inomata H, Watanabe T.et al Clinical utility of thyroid ultrasonography in the diagnosis of congenital hypothyroidism. Endocr J 200249293–297. [DOI] [PubMed] [Google Scholar]
- 20.Marinovic D, Garel C, Czernichow P.et al Additional phenotypic abnormalities with presence of cysts within the empty thyroid area in patients with congenital hypothyroidism with thyroid dysgenesis. J Clin Endocrinol Metab 2003881212–1216. [DOI] [PubMed] [Google Scholar]
- 21.Klingmuller V, Fiedler C, Otten A. Characteristics of thyroid sonography in infants and children. Radiologe 199232320–326. [PubMed] [Google Scholar]
- 22.Chanoine J P, Toppet V, Lagasse R.et al Determination of thyroid volume by ultrasound from the neonatal period to late adolescence. Eur J Pediatr 1991150365–369. [DOI] [PubMed] [Google Scholar]
- 23.Perry R J, Hollman A S, Wood A M.et al Ultrasound of the thyroid gland in the newborn: normative data. Arch Dis Child Fetal Neonatal Ed 200287F209–F211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 24.Ueda D, Yoto Y, Sato T. Ultrasonic assessment of the lingual thyroid gland in children. Pediatr Radiol 199828126–128. [DOI] [PubMed] [Google Scholar]
- 25.Ohnishi H, Sato H, Noda H.et al Color Doppler ultrasonography: diagnosis of ectopic thyroid gland in patients with congenital hypothyroidism caused by thyroid dysgenesis. J Clin Endocrinol Metab 2003885145–5149. [DOI] [PubMed] [Google Scholar]
- 26.Siegel M J. Face and neck. In: Siegel MJ, ed. Pediatric sonography. 3rd edn. Philadephia: Lippincott, Williams and Wilkins, 2002151–152.
- 27.van Isselt J W, de Klerk J M, van Rijk P P.et al Comparison of methods for thyroid volume estimation in patients with Graves' disease. Eur J Nucl Med Mol Imaging 200330525–531. [DOI] [PubMed] [Google Scholar]
- 28.British Thyroid Association and Royal College of Physicians Guidelines for the management of thyroid cancer in adults. Royal College of Physicians: London, 2002 [DOI] [PMC free article] [PubMed]