Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1986 Oct;97(2):377–383. doi: 10.1017/s0022172400065463

Further characterization of 41 isolates of adenovirus types 19/37 by serum neutralization and DNA restriction enzyme analysis.

Z D Meng, M L Kennett, S M Rodger, K E Dickson, B N Anderson, I D Gust
PMCID: PMC2083553  PMID: 3023482

Abstract

Forty-one strains of adenovirus type 19/37 (Ad19/37) mainly isolated from patients with keratoconjunctivitis or conjunctivitis between 1974 and 1984 were re-evaluated by serum neutralization (SN), haemagglutination inhibition (HI) and DNA restriction analysis. Of 19 isolates which were neutralized to high titre by antiserum prepared against prototype Ad19, 5 showed cross-reactivity with 32-64 units of Ad37 antiserum, while of 22 strains neutralized by high titre by Ad37 antiserum, 3 showed cross-reactivity with 32 units of Ad19 antiserum. By DNA restriction analysis, all Ad19 isolates were identical to each other and to Ad19A virus. Using endonuclease Bgl 1, three variants were observed among the Ad37 isolates.

Full text

PDF
377

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki K., Kanazono N., Ishi K., Kato K., Ohtsuka H. [Clinico-epidemiological study of keratoconjunctivitis due to adenovirus type 37 (Ad 37) in Sapporo, Japan]. Nippon Ganka Gakkai Zasshi. 1985 Feb;89(2):294–298. [PubMed] [Google Scholar]
  2. Bell E. J., Winton F. W. Letter: Keratoconjunctivitis caused by adenovirus type 19. Br Med J. 1975 Jan 11;1(5949):91–91. doi: 10.1136/bmj.1.5949.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desmyter J., De Jong J. C., Slaterus K. W., Verlaeckt H. Letter: Keratoconjunctivitis caused by Adenovirus Type 19. Br Med J. 1974 Nov 16;4(5941):406–406. doi: 10.1136/bmj.4.5941.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guyer B., O'Day D. M., Hierholzer J. C., Schaffner W. Epidemic keratoconjunctivitis: a community outbreak of mixed adenovirus type 8 and type 19 infection. J Infect Dis. 1975 Aug;132(2):142–150. doi: 10.1093/infdis/132.2.142. [DOI] [PubMed] [Google Scholar]
  5. Hammond G. W., Mauthe G., Joshua J., Hannan C. K. Examination of uncommon clinical isolates of human adenoviruses by restriction endonuclease analysis. J Clin Microbiol. 1985 Apr;21(4):611–616. doi: 10.1128/jcm.21.4.611-616.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hierholzer J. C., Guyer B., O'Day D., Schaffner W. Letter: Adenovirus type 19 keratoconjunctivitis. N Engl J Med. 1974 Jun 20;290(25):1436–1436. doi: 10.1056/nejm197406202902512. [DOI] [PubMed] [Google Scholar]
  7. Irving L. G., Smith F. A. One-year survey of enteroviruses, adenoviruses, and reoviruses isolated from effluent at an activated-sludge purification plant. Appl Environ Microbiol. 1981 Jan;41(1):51–59. doi: 10.1128/aem.41.1.51-59.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Irving L., Kennett M., Lewis F., Birch C., Donaldson A. Adenovirus eye infections in an Australian city, 1972-9. J Hyg (Lond) 1981 Feb;86(1):95–103. doi: 10.1017/s0022172400068789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keenlyside R. A., Hierholzer J. C., D'Angelo L. J. Keratoconjunctivitis associated with adenovirus type 37: an extended outbreak in an ophthalmologist's office. J Infect Dis. 1983 Feb;147(2):191–198. doi: 10.1093/infdis/147.2.191. [DOI] [PubMed] [Google Scholar]
  10. Kemp M. C., Hierholzer J. C., Cabradilla C. P., Obijeski J. F. The changing etiology of epidemic keratoconjunctivitis: antigenic and restriction enzyme analyses of adenovirus types 19 and 37 isolated over a 10-year period. J Infect Dis. 1983 Jul;148(1):24–33. doi: 10.1093/infdis/148.1.24. [DOI] [PubMed] [Google Scholar]
  11. Schaap G. J., de Jong J. C., van Bijsterveld O. P., Beekhuis W. H. A new intermediate adenovirus type causing conjunctivitis. Arch Ophthalmol. 1979 Dec;97(12):2336–2338. doi: 10.1001/archopht.1979.01020020552010. [DOI] [PubMed] [Google Scholar]
  12. Shinagawa M., Matsuda A., Ishiyama T., Goto H., Sato G. A rapid and simple method for preparation of adenovirus DNA from infected cells. Microbiol Immunol. 1983;27(9):817–822. doi: 10.1111/j.1348-0421.1983.tb00638.x. [DOI] [PubMed] [Google Scholar]
  13. Wadell G., Sundell G., de Jong J. C. Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping. J Med Virol. 1981;7(2):119–125. doi: 10.1002/jmv.1890070205. [DOI] [PubMed] [Google Scholar]
  14. Wadell G., de Jong J. C. Restriction endonucleases in identification of a genome type of adenovirus 19 associated with keratoconjunctivitis. Infect Immun. 1980 Feb;27(2):292–296. doi: 10.1128/iai.27.2.292-296.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. de Jong J. C., Wigand R., Wadell G., Keller D., Muzerie C. J., Wermenbol A. G., Schaap G. J. Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J Med Virol. 1981;7(2):105–118. doi: 10.1002/jmv.1890070204. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES