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. 1991 Oct;173(19):6174–6183. doi: 10.1128/jb.173.19.6174-6183.1991

Isolation and characterization of a new class of cytochrome d terminal oxidase mutants of Escherichia coli.

K L Oden 1, R B Gennis 1
PMCID: PMC208368  PMID: 1655701

Abstract

Cytochrome d terminal oxidase mutants were isolated by using hydroxylamine mutagenesis of pNG2, a pBR322-derived plasmid containing the wild-type cyd operon. The mutagenized plasmid was transformed into a cyo cyd recA strain, and the transformants were screened for the inability to confer aerobic growth on nonfermentable carbon sources. Western blot analysis and visible-light spectroscopy were performed to characterize three independent mutants grown both aerobically and anaerobically. The mutational variants of the cytochrome d complex were stabilized under anaerobic growth conditions. All three mutations perturb the b595 and d heme components of the complex. These mutations were mapped and sequenced and are shown to be located in the N-terminal third of subunit II of the cytochrome d complex. It is proposed that the N terminus of subunit II may interact with subunit I to form an interface that binds the b595 and d heme centers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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