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. 1990 Jan;172(1):136–142. doi: 10.1128/jb.172.1.136-142.1990

Cell-associated oligosaccharides of Bradyrhizobium spp.

K J Miller 1, R S Gore 1, R Johnson 1, A J Benesi 1, V N Reinhold 1
PMCID: PMC208410  PMID: 2294083

Abstract

We report the initial characterization of the cell-associated oligosaccharides produced by four Bradyrhizobium strains: Bradyrhizobium japonicum USDA 110, USDA 94, and ATCC 10324 and Bradyrhizobium sp. strain 32H1. The cell-associated oligosaccharides of these strains were found to be composed solely of glucose and were predominantly smaller than the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species. Linkage studies and nuclear magnetic resonance analyses demonstrated that the bradyrhizobial glucans are linked primarily by beta-1,6 and beta-1,3 glycosidic bonds. Thus, the bradyrhizobia appear to synthesize cell-associated oligosaccharides of structural character substantially different from that of the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species.

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Selected References

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