Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Jan;172(1):299–304. doi: 10.1128/jb.172.1.299-304.1990

Formation of UDP-2-acetamido-2-deoxy-L-galactose and UDP-2-acetamido-2-deoxy-L-galacturonic acid by Pseudomonas aeruginosa.

S Singh 1, U Singh 1, S E Hogan 1, D S Feingold 1
PMCID: PMC208432  PMID: 2152905

Abstract

The O-specific polysaccharide from the lipopolysaccharide of Pseudomonas aeruginosa NCTC 8505 (IATS serotype O:3) consists of a tetrasaccharide repeating unit comprising L-rhamnose, N-acetyl-D-glucosamine (GlcNAc), bacillosamine, and N-acetyl-L-galactosaminuronic acid (L-GalNAcA) (Y. Tahara and S. G. Wilkinson, Eur. J. Biochem. 134:299-304, 1983). Incubation of GlcN or UDP-GlcNAc with cell extracts or EDTA-treated cells of P. aeruginosa NCTC 8505 yielded a mixture of UDP-ManNAc, UDP-GalNAc, UDP-GlcNAcA, UDP-ManNAcA, UDP-L-GalNAc, and UDP-L-GalNAcA. The last two compounds, here identified for the first time, may be intermediates in the synthesis of the L-GalNAcA moiety of the O-specific portion of the lipopolysaccharide of P. aeruginosa.

Full text

PDF
299

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVIGAD G., AMARAL D., ASENSIO C., HORECKER B. L. The D-galactose oxidase of Polyporus circinatus. J Biol Chem. 1962 Sep;237:2736–2743. [PubMed] [Google Scholar]
  2. Barber G. A. Observations on the mechanism of the reversible epimerization of GDP-D-mannose to GDP-L-galactose by an enzyme from Chlorella pyrenoidosa. J Biol Chem. 1979 Aug 25;254(16):7600–7603. [PubMed] [Google Scholar]
  3. Biely P., Jeanloz R. W. The isolation and structure identification of uridine 5-(2-acetamido-2-deoxy-alpha-D-glucopyranosyluronic acid pyrophosphate) from Micrococcus lysodeikticus cells. J Biol Chem. 1969 Sep 25;244(18):4929–4935. [PubMed] [Google Scholar]
  4. Bochner B. R., Ames B. N. Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography. J Biol Chem. 1982 Aug 25;257(16):9759–9769. [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. García R. C., Recondo E., Dankert M. Polysaccharide biosynthesis in Acetobacter xylinum. Enzymatic synthesis of lipid diphosphate and monophospate sugars. Eur J Biochem. 1974 Mar 15;43(1):93–105. doi: 10.1111/j.1432-1033.1974.tb03389.x. [DOI] [PubMed] [Google Scholar]
  7. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  8. Kawamura T., Ichihara N., Sugiyama S., Yokota H., Ishimoto N., Ito E. Biosynthesis of UDP-N-acetyl-D-glucosaminuronic acid and UDP-N-acetyl-D-mannosaminuronic acid in Micrococcus luteus. J Biochem. 1985 Jul;98(1):105–116. doi: 10.1093/oxfordjournals.jbchem.a135248. [DOI] [PubMed] [Google Scholar]
  9. Kaya S., Araki Y., Ito E. The structure of the O-polysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585). J Biochem. 1989 Jan;105(1):35–38. doi: 10.1093/oxfordjournals.jbchem.a122613. [DOI] [PubMed] [Google Scholar]
  10. Knirel Y. A., Vinogradov E. V., Shashkov A. S., Dmitriev B. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chains of Ps.aeruginosa O:2 (Lanyi) lipopolysaccharides. Eur J Biochem. 1982 Jun 15;125(1):221–227. doi: 10.1111/j.1432-1033.1982.tb06672.x. [DOI] [PubMed] [Google Scholar]
  11. Lang L., Kornfeld S. A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine. Anal Biochem. 1984 Jul;140(1):264–269. doi: 10.1016/0003-2697(84)90163-5. [DOI] [PubMed] [Google Scholar]
  12. Shibaev V. N. Biosynthesis of bacterial polysaccharide chains composed of repeating units. Adv Carbohydr Chem Biochem. 1986;44:277–339. doi: 10.1016/s0065-2318(08)60080-3. [DOI] [PubMed] [Google Scholar]
  13. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  14. Tahara Y., Wilkinson S. G. The lipopolysaccharide from Pseudomonas aeruginosa NCTC 8505. Structure of the O-specific polysaccharide. Eur J Biochem. 1983 Aug 1;134(2):299–304. doi: 10.1111/j.1432-1033.1983.tb07565.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES