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. 1990 Jan;172(1):310–316. doi: 10.1128/jb.172.1.310-316.1990

Sequence analysis of the inversion region containing the pilin genes of Moraxella bovis.

K A Fulks 1, C F Marrs 1, S P Stevens 1, M R Green 1
PMCID: PMC208434  PMID: 2403542

Abstract

Moraxella bovis EPP63 is able to produce two antigenically distinct pili called Q and I pili (previously called beta and alpha pili). Hybridization studies have shown that the transition between the types is due to inversion of a 2.1-kilobase segment of chromosomal DNA. We present the sequence of a 4.1-kilobase region of cloned DNA spanning the entire inversion region in orientation 1 (Q pilin expressed). Comparison of this sequence with the sequence of the polymerase chain reaction-amplified genomic DNA from orientation 2 (I pilin expressed) allows the site-specific region of recombination to be localized to a 26-base-pair region in which sequence similarity to the left inverted repeat of the Salmonella typhimurium hin system was previously noted. In addition, 50% sequence similarity was seen in a 60-base-pair segment of our sequence to the recombinational enhancer of bacteriophage P1, an inversion system related to the hin system of S. typhimurium. Finally, two open reading frames representing potential genes were identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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