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. 1990 Jan;172(1):507–510. doi: 10.1128/jb.172.1.507-510.1990

Neurospora endo-exonuclease is immunochemically related to the recC gene product of Escherichia coli.

M J Fraser 1, H Koa 1, T Y Chow 1
PMCID: PMC208465  PMID: 2152915

Abstract

Immunochemical cross-reaction between the endo-exonuclease of Neurospora crassa, an enzyme previously implicated in recombination and recombinational DNA repair, and the recC-encoded polypeptide of Escherichia coli has been detected by immunoblotting extracts of strains of E. coli having a deletion that includes the recBCD genes but carrying multicopy plasmids bearing all three of the recBCD genes or only one or two of these genes. It was predicted that homology would also be found at the amino acid sequence level between the recC polypeptide and both nuclear and mitochondrial endo-exonucleases of Saccharomyces cerevisiae, which cross-react with antibodies raised to the N. crassa endo-exonuclease. Since the gene for the S. cerevisiae mitochondrial enzyme, NUC1, has been cloned and sequenced and the predicted amino acid sequence is known, this sequence was aligned with the predicted amino acid sequence of the recC polypeptide. Extensive homology was found by aligning 306 of the 329 amino acids of the yeast mitochondrial nuclease sequence with the carboxy-terminal one-quarter of the amino acid sequence of the recC polypeptide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almasan A., Mishra N. C. Molecular characterization of the mitochondrial DNA of a new stopper mutant ER-3 of Neurospora crassa. Genetics. 1988 Dec;120(4):935–945. doi: 10.1093/genetics/120.4.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbour S. D., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes. Proc Natl Acad Sci U S A. 1970 Apr;65(4):955–961. doi: 10.1073/pnas.65.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chaudhury A. M., Smith G. R. Escherichia coli recBC deletion mutants. J Bacteriol. 1984 Nov;160(2):788–791. doi: 10.1128/jb.160.2.788-791.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chow T. Y., Fraser M. J. Purification and properties of single strand DNA-binding endo-exonuclease of Neurospora crassa. J Biol Chem. 1983 Oct 10;258(19):12010–12018. [PubMed] [Google Scholar]
  5. Chow T. Y., Resnick M. A. An endo-exonuclease activity of yeast that requires a functional RAD52 gene. Mol Gen Genet. 1988 Jan;211(1):41–48. doi: 10.1007/BF00338391. [DOI] [PubMed] [Google Scholar]
  6. Chow T. Y., Resnick M. A. Purification and characterization of an endo-exonuclease from Saccharomyces cerevisiae that is influenced by the RAD52 gene. J Biol Chem. 1987 Dec 25;262(36):17659–17667. [PubMed] [Google Scholar]
  7. Dake E., Hofmann T. J., McIntire S., Hudson A., Zassenhaus H. P. Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae. J Biol Chem. 1988 Jun 5;263(16):7691–7702. [PubMed] [Google Scholar]
  8. Finch P. W., Storey A., Brown K., Hickson I. D., Emmerson P. T. Complete nucleotide sequence of recD, the structural gene for the alpha subunit of Exonuclease V of Escherichia coli. Nucleic Acids Res. 1986 Nov 11;14(21):8583–8594. doi: 10.1093/nar/14.21.8583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Finch P. W., Storey A., Chapman K. E., Brown K., Hickson I. D., Emmerson P. T. Complete nucleotide sequence of the Escherichia coli recB gene. Nucleic Acids Res. 1986 Nov 11;14(21):8573–8582. doi: 10.1093/nar/14.21.8573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Finch P. W., Wilson R. E., Brown K., Hickson I. D., Tomkinson A. E., Emmerson P. T. Complete nucleotide sequence of the Escherichia coli recC gene and of the thyA-recC intergenic region. Nucleic Acids Res. 1986 Jun 11;14(11):4437–4451. doi: 10.1093/nar/14.11.4437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fraser M. J., Chow T. Y., Cohen H., Koa H. An immunochemical study of Neurospora nucleases. Biochem Cell Biol. 1986 Feb;64(2):106–116. doi: 10.1139/o86-018. [DOI] [PubMed] [Google Scholar]
  12. Fraser M. J., Cohen H. Intracellular localization of Neurospora crassa endo-exonuclease and its putative precursor. J Bacteriol. 1983 Apr;154(1):460–470. doi: 10.1128/jb.154.1.460-470.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fraser M. J., Hatahet Z., Huang X. T. The actions of Neurospora endo-exonuclease on double strand DNAs. J Biol Chem. 1989 Aug 5;264(22):13093–13101. [PubMed] [Google Scholar]
  14. Goldmark P. J., Linn S. Purification and properties of the recBC DNase of Escherichia coli K-12. J Biol Chem. 1972 Mar 25;247(6):1849–1860. [PubMed] [Google Scholar]
  15. Ramotar D., Auchincloss A. H., Fraser M. J. Nuclear endo-exonuclease of Neurospora crassa. Evidence for a role in DNA repair. J Biol Chem. 1987 Jan 5;262(1):425–431. [PubMed] [Google Scholar]
  16. Smith G. R. Homologous recombination in procaryotes. Microbiol Rev. 1988 Mar;52(1):1–28. doi: 10.1128/mr.52.1.1-28.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomkinson A. E., Linn S. Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria. Nucleic Acids Res. 1986 Dec 22;14(24):9579–9593. doi: 10.1093/nar/14.24.9579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vincent R. D., Hofmann T. J., Zassenhaus H. P. Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae. Nucleic Acids Res. 1988 Apr 25;16(8):3297–3312. doi: 10.1093/nar/16.8.3297. [DOI] [PMC free article] [PubMed] [Google Scholar]

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