Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Feb;172(2):648–652. doi: 10.1128/jb.172.2.648-652.1990

Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cells.

D Cavard 1, C Lazdunski 1
PMCID: PMC208488  PMID: 2404946

Abstract

Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins. On the other hand, two colicin A recombinants were split after release from the wild-type bacteria that produced them but not from ompT mutant cells.

Full text

PDF
648

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baty D., Lloubès R., Geli V., Lazdunski C., Howard S. P. Extracellular release of colicin A is non-specific. EMBO J. 1987 Aug;6(8):2463–2468. doi: 10.1002/j.1460-2075.1987.tb02526.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowles L. K., Konisky J. Cleavage of colicin Ia by the Escherichia coli K-12 outer membrane is not mediated by the colicin Ia receptor. J Bacteriol. 1981 Jan;145(1):668–671. doi: 10.1128/jb.145.1.668-671.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brey R. N. Fragmentation of colicins A and E1 by cell surface proteases. J Bacteriol. 1982 Jan;149(1):306–315. doi: 10.1128/jb.149.1.306-315.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caron P. R., Grossman L. Potential role of proteolysis in the control of UvrABC incision. Nucleic Acids Res. 1988 Oct 25;16(20):9641–9650. doi: 10.1093/nar/16.20.9641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cavard D., Crozel V., Gorvel J. P., Pattus F., Baty D., Lazdunski C. A molecular, genetic and immunological approach to the functioning of colicin A, a pore-forming protein. J Mol Biol. 1986 Feb 5;187(3):449–459. doi: 10.1016/0022-2836(86)90445-6. [DOI] [PubMed] [Google Scholar]
  6. Cavard D., Howard S. P., Lazdunski C. Functioning of the colicin A lysis protein is affected by Triton X-100, divalent cations and EDTA. J Gen Microbiol. 1989 Jun;135(6):1715–1726. doi: 10.1099/00221287-135-6-1715. [DOI] [PubMed] [Google Scholar]
  7. Cavard D., Howard S. P., Lloubes R., Lazdunski C. High-level expression of the colicin A lysis protein. Mol Gen Genet. 1989 Jun;217(2-3):511–519. doi: 10.1007/BF02464925. [DOI] [PubMed] [Google Scholar]
  8. Cavard D., Pages J. M., Lazdunski C. J. A protease as a possible sensor of environmental conditions in E. coli outer membrane. Mol Gen Genet. 1982;188(3):508–512. doi: 10.1007/BF00330057. [DOI] [PubMed] [Google Scholar]
  9. Chung C. T., Miller R. H. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res. 1988 Apr 25;16(8):3580–3580. doi: 10.1093/nar/16.8.3580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cole S. T., Saint-Joanis B., Pugsley A. P. Molecular characterisation of the colicin E2 operon and identification of its products. Mol Gen Genet. 1985;198(3):465–472. doi: 10.1007/BF00332940. [DOI] [PubMed] [Google Scholar]
  11. Fiss E. H., Hollifield W. C., Jr, Neilands J. B. Absence of ferric enterobactin receptor modification activity in mutants of Escherichia coli K-12 lacking protein a. Biochem Biophys Res Commun. 1979 Nov 14;91(1):29–34. doi: 10.1016/0006-291x(79)90578-3. [DOI] [PubMed] [Google Scholar]
  12. Grodberg J., Dunn J. J. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification. J Bacteriol. 1988 Mar;170(3):1245–1253. doi: 10.1128/jb.170.3.1245-1253.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grodberg J., Lundrigan M. D., Toledo D. L., Mangel W. F., Dunn J. J. Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12. Nucleic Acids Res. 1988 Feb 11;16(3):1209–1209. doi: 10.1093/nar/16.3.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Knibiehler M., Howard S. P., Baty D., Geli V., Lloubès R., Sauve P., Lazdunski C. Isolation and molecular and functional properties of the amino-terminal domain of colicin A. Eur J Biochem. 1989 Apr 15;181(1):109–113. doi: 10.1111/j.1432-1033.1989.tb14700.x. [DOI] [PubMed] [Google Scholar]
  15. Knibiehler M., Lazdunski C. Conformation of colicin A: apparent difference between cytoplasmic and extracellular polypeptide chain. FEBS Lett. 1987 Jun 1;216(2):183–189. doi: 10.1016/0014-5793(87)80686-5. [DOI] [PubMed] [Google Scholar]
  16. Krone W. J., de Vries P., Koningstein G., de Jonge A. J., de Graaf F. K., Oudega B. Uptake of cloacin DF13 by susceptible cells: removal of immunity protein and fragmentation of cloacin molecules. J Bacteriol. 1986 Apr;166(1):260–268. doi: 10.1128/jb.166.1.260-268.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lazdunski C. J. Pore-forming colicins: synthesis, extracellular release, mode of action, immunity. Biochimie. 1988 Sep;70(9):1291–1296. doi: 10.1016/0300-9084(88)90197-6. [DOI] [PubMed] [Google Scholar]
  18. Masaki H., Ohta T. Colicin E3 and its immunity genes. J Mol Biol. 1985 Mar 20;182(2):217–227. doi: 10.1016/0022-2836(85)90340-7. [DOI] [PubMed] [Google Scholar]
  19. Morlon J., Lloubès R., Varenne S., Chartier M., Lazdunski C. Complete nucleotide sequence of the structural gene for colicin A, a gene translated at non-uniform rate. J Mol Biol. 1983 Oct 25;170(2):271–285. doi: 10.1016/s0022-2836(83)80148-x. [DOI] [PubMed] [Google Scholar]
  20. Ohno-Iwashita Y., Imahori K. Assignment of the functional loci in colicin E2 and E3 molecules by the characterization of their proteolytic fragments. Biochemistry. 1980 Feb 19;19(4):652–659. doi: 10.1021/bi00545a008. [DOI] [PubMed] [Google Scholar]
  21. Pugsley A. P. Colicin E4-CT9 is proteolytically degraded after discharge from producing cells in liquid cultures. J Gen Microbiol. 1983 Mar;129(3):833–840. doi: 10.1099/00221287-129-3-833. [DOI] [PubMed] [Google Scholar]
  22. Pugsley A. P. The ins and outs of colicins. Part I: Production, and translocation across membranes. Microbiol Sci. 1984 Oct;1(7):168–175. [PubMed] [Google Scholar]
  23. Sedgwick B. In vitro proteolytic cleavage of the Escherichia coli Ada protein by the ompT gene product. J Bacteriol. 1989 Apr;171(4):2249–2251. doi: 10.1128/jb.171.4.2249-2251.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sugimura K., Nishihara T. Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT. J Bacteriol. 1988 Dec;170(12):5625–5632. doi: 10.1128/jb.170.12.5625-5632.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yamada M., Ebina Y., Miyata T., Nakazawa T., Nakazawa A. Nucleotide sequence of the structural gene for colicin E1 and predicted structure of the protein. Proc Natl Acad Sci U S A. 1982 May;79(9):2827–2831. doi: 10.1073/pnas.79.9.2827. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES