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. 1990 Feb;172(2):735–740. doi: 10.1128/jb.172.2.735-740.1990

Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis.

F M Hulett 1, C Bookstein 1, K Jensen 1
PMCID: PMC208500  PMID: 2105301

Abstract

Two secreted alkaline phosphatase proteins were purified from cultures of Bacillus subtilis JH646MS. The two proteins showed slight differences in subunit molecular weight, substrate specificity, and charge characteristics. A total of 62% of the first 22 amino-terminal amino acids were identical. Both sequences showed conservation of structural features identified in Escherichia coli and human alkaline phosphatases. One alkaline phosphatase was a monomer and the other was a dimer. Southern analysis of genomic DNA with degenerative oligomers based on the amino acid sequences suggest that there are two structural genes for alkaline phosphatase in the genome of B. subtilis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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