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. 1990 Feb;172(2):1133–1136. doi: 10.1128/jb.172.2.1133-1136.1990

Regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by cyclic AMP-dependent protein kinase.

A J Kinney 1, M Bae-Lee 1, S S Panghaal 1, M J Kelley 1, P M Gaynor 1, G M Carman 1
PMCID: PMC208547  PMID: 2153654

Abstract

The addition of cyclic AMP (cAMP) to Saccharomyces cerevisiae cyr1 mutant cells resulted in an increase in the rate of phosphatidylinositol synthesis at the expense of phosphatidylserine synthesis. The decrease in phosphatidylserine synthesis correlated with the down regulation of phosphatidylserine synthase activity by cAMP-dependent protein kinase phosphorylation. The increase in phosphatidylinositol synthesis was not due to the regulation of phosphatidylinositol synthase by cAMP-dependent protein kinase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailis A. M., Poole M. A., Carman G. M., Henry S. A. The membrane-associated enzyme phosphatidylserine synthase is regulated at the level of mRNA abundance. Mol Cell Biol. 1987 Jan;7(1):167–176. doi: 10.1128/mcb.7.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carman G. M., Henry S. A. Phospholipid biosynthesis in yeast. Annu Rev Biochem. 1989;58:635–669. doi: 10.1146/annurev.bi.58.070189.003223. [DOI] [PubMed] [Google Scholar]
  3. Carman G. M., Matas J. Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae. Can J Microbiol. 1981 Nov;27(11):1140–1149. doi: 10.1139/m81-179. [DOI] [PubMed] [Google Scholar]
  4. Culbertson M. R., Henry S. A. Inositol-requiring mutants of Saccharomyces cerevisiae. Genetics. 1975 May;80(1):23–40. doi: 10.1093/genetics/80.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fischl A. S., Carman G. M. Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase. J Bacteriol. 1983 Apr;154(1):304–311. doi: 10.1128/jb.154.1.304-311.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fischl A. S., Homann M. J., Poole M. A., Carman G. M. Phosphatidylinositol synthase from Saccharomyces cerevisiae. Reconstitution, characterization, and regulation of activity. J Biol Chem. 1986 Mar 5;261(7):3178–3183. [PubMed] [Google Scholar]
  7. Henry S. A., Atkinson K. D., Kolat A. I., Culbertson M. R. Growth and metabolism of inositol-starved Saccharomyces cerevisiae. J Bacteriol. 1977 Apr;130(1):472–484. doi: 10.1128/jb.130.1.472-484.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Homann M. J., Poole M. A., Gaynor P. M., Ho C. T., Carman G. M. Effect of growth phase on phospholipid biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1987 Feb;169(2):533–539. doi: 10.1128/jb.169.2.533-539.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson K. E., Cameron S., Toda T., Wigler M., Zoller M. J. Expression in Escherichia coli of BCY1, the regulatory subunit of cyclic AMP-dependent protein kinase from Saccharomyces cerevisiae. Purification and characterization. J Biol Chem. 1987 Jun 25;262(18):8636–8642. [PubMed] [Google Scholar]
  10. Kelley M. J., Bailis A. M., Henry S. A., Carman G. M. Regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by inositol. Inositol is an inhibitor of phosphatidylserine synthase activity. J Biol Chem. 1988 Dec 5;263(34):18078–18085. [PubMed] [Google Scholar]
  11. Kelley M. J., Carman G. M. Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae. J Biol Chem. 1987 Oct 25;262(30):14563–14570. [PubMed] [Google Scholar]
  12. Kinney A. J., Carman G. M. Phosphorylation of yeast phosphatidylserine synthase in vivo and in vitro by cyclic AMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7962–7966. doi: 10.1073/pnas.85.21.7962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klig L. S., Homann M. J., Carman G. M., Henry S. A. Coordinate regulation of phospholipid biosynthesis in Saccharomyces cerevisiae: pleiotropically constitutive opi1 mutant. J Bacteriol. 1985 Jun;162(3):1135–1141. doi: 10.1128/jb.162.3.1135-1141.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Krebs E. G., Beavo J. A. Phosphorylation-dephosphorylation of enzymes. Annu Rev Biochem. 1979;48:923–959. doi: 10.1146/annurev.bi.48.070179.004423. [DOI] [PubMed] [Google Scholar]
  15. Letts V. A., Henry S. A. Regulation of phospholipid synthesis in phosphatidylserine synthase-deficient (chol) mutants of Saccharomyces cerevisiae. J Bacteriol. 1985 Aug;163(2):560–567. doi: 10.1128/jb.163.2.560-567.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Letts V. A., Klig L. S., Bae-Lee M., Carman G. M., Henry S. A. Isolation of the yeast structural gene for the membrane-associated enzyme phosphatidylserine synthase. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7279–7283. doi: 10.1073/pnas.80.23.7279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Matsumoto K., Uno I., Ishikawa T. Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase. Cell. 1983 Feb;32(2):417–423. doi: 10.1016/0092-8674(83)90461-0. [DOI] [PubMed] [Google Scholar]
  18. Matsumoto K., Uno I., Oshima Y., Ishikawa T. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2355–2359. doi: 10.1073/pnas.79.7.2355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Otaka E., Kumazaki T., Matsumoto K. In vivo phosphorylation of Saccharomyces cerevisiae ribosomal protein S10 by cyclic-AMP-dependent protein kinase. J Bacteriol. 1986 Aug;167(2):713–715. doi: 10.1128/jb.167.2.713-715.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Poole M. A., Homann M. J., Bae-Lee M. S., Carman G. M. Regulation of phosphatidylserine synthase from Saccharomyces cerevisiae by phospholipid precursors. J Bacteriol. 1986 Nov;168(2):668–672. doi: 10.1128/jb.168.2.668-672.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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