Abstract
During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of protein synthesis uniquely induces the heat shock response. Several hours later, lambda prophage was induced and the cells lysed.
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- Bahl H., Echols H., Straus D. B., Court D., Crowl R., Georgopoulos C. P. Induction of the heat shock response of E. coli through stabilization of sigma 32 by the phage lambda cIII protein. Genes Dev. 1987 Mar;1(1):57–64. doi: 10.1101/gad.1.1.57. [DOI] [PubMed] [Google Scholar]
- Blumenthal R. M., Reeh S., Pedersen S. Regulation of transcription factor rho and the alpha subunit of RNA polymerase in Escherichia coli B/r. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2285–2288. doi: 10.1073/pnas.73.7.2285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bothwell A. L., Garber R. L., Altman S. Nucleotide sequence and in vitro processing of a precursor molecule to Escherichia coli 4.5 S RNA. J Biol Chem. 1976 Dec 10;251(23):7709–7716. [PubMed] [Google Scholar]
- Bourgaize D. B., Fournier M. J. Initiation of translation is impaired in E. coli cells deficient in 4.5S RNA. Nature. 1987 Jan 15;325(6101):281–284. doi: 10.1038/325281a0. [DOI] [PubMed] [Google Scholar]
- Brown S., Fournier M. J. The 4.5 S RNA gene of Escherichia coli is essential for cell growth. J Mol Biol. 1984 Sep 25;178(3):533–550. doi: 10.1016/0022-2836(84)90237-7. [DOI] [PubMed] [Google Scholar]
- Brown S. Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli. Cell. 1987 Jun 19;49(6):825–833. doi: 10.1016/0092-8674(87)90620-9. [DOI] [PubMed] [Google Scholar]
- Brown S. Time of action of 4.5 S RNA in Escherichia coli translation. J Mol Biol. 1989 Sep 5;209(1):79–90. doi: 10.1016/0022-2836(89)90171-x. [DOI] [PubMed] [Google Scholar]
- Goff S. A., Goldberg A. L. Production of abnormal proteins in E. coli stimulates transcription of lon and other heat shock genes. Cell. 1985 Jun;41(2):587–595. doi: 10.1016/s0092-8674(85)80031-3. [DOI] [PubMed] [Google Scholar]
- Griffin B. E. Studies and sequences of Escherichia coli 4.5 S RNA. J Biol Chem. 1975 Jul 25;250(14):5426–5437. [PubMed] [Google Scholar]
- Hsu L. M., Zagorski J., Fournier M. J. Cloning and sequence analysis of the Escherichia coli 4.5 S RNA gene. J Mol Biol. 1984 Sep 25;178(3):509–531. doi: 10.1016/0022-2836(84)90236-5. [DOI] [PubMed] [Google Scholar]
- Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. II. Noncoordinate accumulation during stringent control. J Biol Chem. 1973 Jul 25;248(14):5033–5041. [PubMed] [Google Scholar]
- Ikemura T., Shimura Y., Sakano H., Ozeki H. Precursor molecules of Escherichia coli transfer RNAs accumulated in a temperature-sensitive mutant. J Mol Biol. 1975 Jul 25;96(1):69–86. doi: 10.1016/0022-2836(75)90182-5. [DOI] [PubMed] [Google Scholar]
- Lee S. Y., Bailey S. C., Apirion D. Small stable RNAs from Escherichia coli: evidence for the existence of new molecules and for a new ribonucleoprotein particle containing 6S RNA. J Bacteriol. 1978 Feb;133(2):1015–1023. doi: 10.1128/jb.133.2.1015-1023.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Straus D. B., Walter W. A., Gross C. A. The heat shock response of E. coli is regulated by changes in the concentration of sigma 32. Nature. 1987 Sep 24;329(6137):348–351. doi: 10.1038/329348a0. [DOI] [PubMed] [Google Scholar]
- Tilly K., Erickson J., Sharma S., Georgopoulos C. Heat shock regulatory gene rpoH mRNA level increases after heat shock in Escherichia coli. J Bacteriol. 1986 Dec;168(3):1155–1158. doi: 10.1128/jb.168.3.1155-1158.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VanBogelen R. A., Kelley P. M., Neidhardt F. C. Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli. J Bacteriol. 1987 Jan;169(1):26–32. doi: 10.1128/jb.169.1.26-32.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wanner B. L., Kodaira R., Neidhardt F. C. Physiological regulation of a decontrolled lac operon. J Bacteriol. 1977 Apr;130(1):212–222. doi: 10.1128/jb.130.1.212-222.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]