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. 1990 Mar;172(3):1436–1440. doi: 10.1128/jb.172.3.1436-1440.1990

Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.

V François 1, A Conter 1, J M Louarn 1
PMCID: PMC208617  PMID: 2155201

Abstract

Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for the plasmid-chromosome fusion.

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Selected References

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  1. Berg C. M., Liu L., Wang B., Wang M. D. Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids. J Bacteriol. 1988 Jan;170(1):468–470. doi: 10.1128/jb.170.1.468-470.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernardi A., Bernardi F. Complete sequence of an IS element present in pSC101. Nucleic Acids Res. 1981 Jun 25;9(12):2905–2911. doi: 10.1093/nar/9.12.2905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cesareni G., Muesing M. A., Polisky B. Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6313–6317. doi: 10.1073/pnas.79.20.6313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chandler M., Roulet E., Silver L., Boy de la Tour E., Caro L. Tn10 mediated integration of the plasmid R100.1 into the bacterial chromosome: inverse transposition. Mol Gen Genet. 1979 May 23;173(1):23–30. doi: 10.1007/BF00267687. [DOI] [PubMed] [Google Scholar]
  6. Chandler M., Silver L., Roth Y., Caro L. Chromosome replication in an Hfr strain of Escherichia coli. J Mol Biol. 1976 Jun 25;104(2):517–523. doi: 10.1016/0022-2836(76)90285-0. [DOI] [PubMed] [Google Scholar]
  7. Churchward G., Linder P., Caro L. The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101. Nucleic Acids Res. 1983 Aug 25;11(16):5645–5659. doi: 10.1093/nar/11.16.5645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fellay R., Frey J., Krisch H. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene. 1987;52(2-3):147–154. doi: 10.1016/0378-1119(87)90041-2. [DOI] [PubMed] [Google Scholar]
  9. Froehlich B. J., Tatti K., Scott J. R. Evidence for positive regulation of plasmid prophage P1 replication: integrative suppression by copy mutants. J Bacteriol. 1983 Oct;156(1):205–211. doi: 10.1128/jb.156.1.205-211.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gamas P., Burger A. C., Churchward G., Caro L., Galas D., Chandler M. Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF. Mol Gen Genet. 1986 Jul;204(1):85–89. doi: 10.1007/BF00330192. [DOI] [PubMed] [Google Scholar]
  11. Guyer M. S., Reed R. R., Steitz J. A., Low K. B. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):135–140. doi: 10.1101/sqb.1981.045.01.022. [DOI] [PubMed] [Google Scholar]
  12. Hamilton C. M., Aldea M., Washburn B. K., Babitzke P., Kushner S. R. New method for generating deletions and gene replacements in Escherichia coli. J Bacteriol. 1989 Sep;171(9):4617–4622. doi: 10.1128/jb.171.9.4617-4622.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Harayama S., Oguchi T., Iino T. The E. coli K-12 chromosome flanked by two IS10 sequences transposes. Mol Gen Genet. 1984;197(1):62–66. doi: 10.1007/BF00327923. [DOI] [PubMed] [Google Scholar]
  14. Hashimoto-Gotoh T., Sekiguchi M. Mutations of temperature sensitivity in R plasmid pSC101. J Bacteriol. 1977 Aug;131(2):405–412. doi: 10.1128/jb.131.2.405-412.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hasunuma K., Sekiguchi M. Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function. Mol Gen Genet. 1977 Sep 9;154(3):225–230. doi: 10.1007/BF00571277. [DOI] [PubMed] [Google Scholar]
  16. Louarn J. M., Bouché J. P., Legendre F., Louarn J., Patte J. Characterization and properties of very large inversions of the E. coli chromosome along the origin-to-terminus axis. Mol Gen Genet. 1985;201(3):467–476. doi: 10.1007/BF00331341. [DOI] [PubMed] [Google Scholar]
  17. Maloy S. R., Nunn W. D. Selection for loss of tetracycline resistance by Escherichia coli. J Bacteriol. 1981 Feb;145(2):1110–1111. doi: 10.1128/jb.145.2.1110-1111.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Raleigh E. A., Kleckner N. Multiple IS10 rearrangements in Escherichia coli. J Mol Biol. 1984 Mar 15;173(4):437–461. doi: 10.1016/0022-2836(84)90390-5. [DOI] [PubMed] [Google Scholar]
  19. Rebollo J. E., François V., Louarn J. M. Detection and possible role of two large nondivisible zones on the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9391–9395. doi: 10.1073/pnas.85.24.9391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Singer M., Baker T. A., Schnitzler G., Deischel S. M., Goel M., Dove W., Jaacks K. J., Grossman A. D., Erickson J. W., Gross C. A. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev. 1989 Mar;53(1):1–24. doi: 10.1128/mr.53.1.1-24.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vocke C., Bastia D. DNA-protein interaction at the origin of DNA replication of the plasmid pSC101. Cell. 1983 Dec;35(2 Pt 1):495–502. doi: 10.1016/0092-8674(83)90183-6. [DOI] [PubMed] [Google Scholar]

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