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. 1990 Mar;172(3):1663–1666. doi: 10.1128/jb.172.3.1663-1666.1990

High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.

A Schäfer 1, J Kalinowski 1, R Simon 1, A H Seep-Feldhaus 1, A Pühler 1
PMCID: PMC208647  PMID: 2106514

Abstract

We report on the mobilization of shuttle plasmids from gram-negative Escherichia coli to gram-positive corynebacteria mediated by P-type transfer functions. Introduction of plasmids into corynebacteria was markedly enhanced after heat treatment of the recipient cells. High-frequency plasmid transfer was also observed when the restriction system of the recipient was mutated. On the basis of our data, we conclude that efficient DNA transfer from gram-negative to gram-positive bacteria, at least to coryneform bacteria, is conceivable in certain natural ecosystems.

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Selected References

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  1. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERTANI G., WEIGLE J. J. Host controlled variation in bacterial viruses. J Bacteriol. 1953 Feb;65(2):113–121. doi: 10.1128/jb.65.2.113-121.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bailey C. R., Winstanley D. J. Inhibition of restriction in Streptomyces clavuligerus by heat treatment. J Gen Microbiol. 1986 Oct;132(10):2945–2947. doi: 10.1099/00221287-132-10-2945. [DOI] [PubMed] [Google Scholar]
  4. Beringer J. E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974 Sep;84(1):188–198. doi: 10.1099/00221287-84-1-188. [DOI] [PubMed] [Google Scholar]
  5. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bousfield I. J. A taxonomic study of some coryneform bacteria. J Gen Microbiol. 1972 Aug;71(3):441–455. doi: 10.1099/00221287-71-3-441. [DOI] [PubMed] [Google Scholar]
  7. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Datta N., Hedges R. W. Host ranges of R factors. J Gen Microbiol. 1972 May;70(3):453–460. doi: 10.1099/00221287-70-3-453. [DOI] [PubMed] [Google Scholar]
  9. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Engel H. W., Soedirman N., Rost J. A., van Leeuwen W. J., van Embden J. D. Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus. J Bacteriol. 1980 May;142(2):407–413. doi: 10.1128/jb.142.2.407-413.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Engel P. Plasmid transformation of Streptomyces tendae after heat attenuation of restriction. Appl Environ Microbiol. 1987 Jan;53(1):1–3. doi: 10.1128/aem.53.1.1-3.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mazodier P., Petter R., Thompson C. Intergeneric conjugation between Escherichia coli and Streptomyces species. J Bacteriol. 1989 Jun;171(6):3583–3585. doi: 10.1128/jb.171.6.3583-3585.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meselson M., Yuan R. DNA restriction enzyme from E. coli. Nature. 1968 Mar 23;217(5134):1110–1114. doi: 10.1038/2171110a0. [DOI] [PubMed] [Google Scholar]
  14. Mojica-A T., Middleton R. B. Fertility of Salmonella typhimurium crosses with Escherichia coli. J Bacteriol. 1971 Dec;108(3):1161–1167. doi: 10.1128/jb.108.3.1161-1167.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olsen R. H., Shipley P. Host range and properties of the Pseudomonas aeruginosa R factor R1822. J Bacteriol. 1973 Feb;113(2):772–780. doi: 10.1128/jb.113.2.772-780.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Satta G., Gudas L. J., Pardee A. B. Degradation of Escherichia coli DNA: evidence for limitation in vivo by protein X, the recA gene product. Mol Gen Genet. 1979 Jan 5;168(1):69–80. doi: 10.1007/BF00267935. [DOI] [PubMed] [Google Scholar]
  17. Seiler H. Identification key for coryneform bacteria derived by numerical taxonomic studies. J Gen Microbiol. 1983 May;129(5):1433–1471. doi: 10.1099/00221287-129-5-1433. [DOI] [PubMed] [Google Scholar]
  18. Simon R., O'Connell M., Labes M., Pühler A. Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria. Methods Enzymol. 1986;118:640–659. doi: 10.1016/0076-6879(86)18106-7. [DOI] [PubMed] [Google Scholar]
  19. Trieu-Cuot P., Carlier C., Courvalin P. Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli. J Bacteriol. 1988 Sep;170(9):4388–4391. doi: 10.1128/jb.170.9.4388-4391.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Volkert M. R., Spencer D. F., Clark A. J. Indirect and intragenic suppression of the lexA102 mutation in E. coli B/r. Mol Gen Genet. 1979;177(1):129–137. doi: 10.1007/BF00267262. [DOI] [PubMed] [Google Scholar]
  21. Willetts N., Crowther C. Mobilization of the non-conjugative IncQ plasmid RSF1010. Genet Res. 1981 Jun;37(3):311–316. doi: 10.1017/s0016672300020310. [DOI] [PubMed] [Google Scholar]

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