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. 1990 Mar;172(3):1663–1666. doi: 10.1128/jb.172.3.1663-1666.1990

High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.

A Schäfer 1, J Kalinowski 1, R Simon 1, A H Seep-Feldhaus 1, A Pühler 1
PMCID: PMC208647  PMID: 2106514

Abstract

We report on the mobilization of shuttle plasmids from gram-negative Escherichia coli to gram-positive corynebacteria mediated by P-type transfer functions. Introduction of plasmids into corynebacteria was markedly enhanced after heat treatment of the recipient cells. High-frequency plasmid transfer was also observed when the restriction system of the recipient was mutated. On the basis of our data, we conclude that efficient DNA transfer from gram-negative to gram-positive bacteria, at least to coryneform bacteria, is conceivable in certain natural ecosystems.

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Selected References

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