Abstract
A pULB113 (RP4::mini-Mu cts) plasmid was used to generate a library of prime plasmids carrying fragments of the Methylobacillus flagellatum genome. The genes carried by these prime plasmids were identified by complementation after transfer to suitably marked Escherichia coli and Pseudomonas aeruginosa strains. The hybrid plasmids were used for complementation mapping with a range of E. coli, M. flagellatum, and P. aeruginosa mutants. A preliminary map of the M. flagellatum genome section with seven groups of linked markers was obtained. Three of seven groups contain an overlapping sequence of cloned genes and can be considered as one large group of linked genes. A high-frequency-of-recombination donor of M. flagellatum (strain MFK64) mobilized the chromosome in a polarized manner from a single transfer origin. The donor was used to construct a time-of-entry map of the M. flagellatum chromosome. This was achieved by determining the time of entry of six randomly dispersed markers, four of which are included in known groups of linked markers. The linear map of M. flagellatum reported here consists of 44 markers.
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- Clark A. J. The beginning of a genetic analysis of recombination proficiency. J Cell Physiol. 1967 Oct;70(2 Suppl):165–180. doi: 10.1002/jcp.1040700412. [DOI] [PubMed] [Google Scholar]
- Haas D., Watson J., Krieg R., Leisinger T. Isolation of an Hfr donor of Pseudomonas aeruginosa PAO by insertion of the plasmid RP1 into the tryptophan synthase gene. Mol Gen Genet. 1981;182(2):240–244. doi: 10.1007/BF00269664. [DOI] [PubMed] [Google Scholar]
- Holloway B. W., Krishnapillai V., Morgan A. F. Chromosomal genetics of Pseudomonas. Microbiol Rev. 1979 Mar;43(1):73–102. doi: 10.1128/mr.43.1.73-102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holloway B. W., Morgan A. F. Genome organization in Pseudomonas. Annu Rev Microbiol. 1986;40:79–105. doi: 10.1146/annurev.mi.40.100186.000455. [DOI] [PubMed] [Google Scholar]
- Howard-Flanders P. Genes that control DNA repair and genetic recombination in Escherichia coli. Adv Biol Med Phys. 1968;12:299–317. doi: 10.1016/b978-1-4831-9928-3.50016-3. [DOI] [PubMed] [Google Scholar]
- Lejeune P., Mergeay M., Van Gijsegem F., Faelen M., Gerits J., Toussaint A. Chromosome transfer and R-prime plasmid formation mediated by plasmid pULB113 (RP4::mini-Mu) in Alcaligenes eutrophus CH34 and Pseudomonas fluorescens 6.2. J Bacteriol. 1983 Sep;155(3):1015–1026. doi: 10.1128/jb.155.3.1015-1026.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore A. T., Nayudu M., Holloway B. W. Genetic mapping in Methylophilus methylotrophus AS1. J Gen Microbiol. 1983 Mar;129(3):785–799. doi: 10.1099/00221287-129-3-785. [DOI] [PubMed] [Google Scholar]
- O'Hoy K., Krishnapillai V. Recalibration of the Pseudomonas aeruginosa strain PAO chromosome map in time units using high-frequency-of-recombination donors. Genetics. 1987 Apr;115(4):611–618. doi: 10.1093/genetics/115.4.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Résibois A., Toussaint A., van Gijsegem F., Faelen M. Physical characterization of mini-mu and mini-D108. Gene. 1981 Jun-Jul;14(1-2):103–113. doi: 10.1016/0378-1119(81)90152-9. [DOI] [PubMed] [Google Scholar]
- Serebrijski I. G., Kazakova S. M., Tsygankov Y. D. Construction of Hfr-like donors of the obligate methanol-oxidizing bacterium Methylobacillus flagellatum KT. FEMS Microbiol Lett. 1989 May;50(1-2):203–206. doi: 10.1016/0378-1097(89)90486-2. [DOI] [PubMed] [Google Scholar]