Abstract
The Escherichia coli F factor mediates conjugal transfer of a plasmid such as pBR322 primarily by replicative transposition of transposon gamma delta (Tn1000) from F to that plasmid to form a cointegrate intermediate. Although resolution of this cointegrate always yields a plasmid containing a single gamma delta insertion, the occasional recovery of transposon-free plasmids after conjugal transfer has led to alternative hypotheses for F mobilization. We show here that gamma delta-free plasmids are found after F-mediated conjugal transfer only when the donor plasmid is a dimer and the recipient is Rec+.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balbás P., Soberón X., Merino E., Zurita M., Lomeli H., Valle F., Flores N., Bolivar F. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene. 1986;50(1-3):3–40. doi: 10.1016/0378-1119(86)90307-0. [DOI] [PubMed] [Google Scholar]
- Bedbrook J. R., Ausubel F. M. Recombination between bacterial plasmids leading to the formation of plasmid multimers. Cell. 1976 Dec;9(4 Pt 2):707–716. doi: 10.1016/0092-8674(76)90134-3. [DOI] [PubMed] [Google Scholar]
- Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berg C. M., Liu L., Coon M., Strausbaugh L. D., Gray P., Vartak N. B., Brown M., Talbot D., Berg D. E. pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138–141. doi: 10.1016/0147-619x(89)90057-7. [DOI] [PubMed] [Google Scholar]
- Goto N., Shoji A., Horiuchi S., Nakaya R. Conduction of nonconjugative plasmids by F' lac is not necessarily associated with transposition of the gamma delta sequence. J Bacteriol. 1984 Aug;159(2):590–596. doi: 10.1128/jb.159.2.590-596.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guyer M. S. The gamma delta sequence of F is an insertion sequence. J Mol Biol. 1978 Dec 15;126(3):347–365. doi: 10.1016/0022-2836(78)90045-1. [DOI] [PubMed] [Google Scholar]
- Guyer M. S. Uses of the transposon gamma delta in the analysis of cloned genes. Methods Enzymol. 1983;101:362–369. doi: 10.1016/0076-6879(83)01027-7. [DOI] [PubMed] [Google Scholar]
- Karlovský P., Vasková M. Tn1000 insertional mutagenesis of cloned repressor gene of the phage L: plasmid oligomerization in the presence of F'lac. Folia Microbiol (Praha) 1987;32(3):185–193. doi: 10.1007/BF02881098. [DOI] [PubMed] [Google Scholar]
- Liu L., Whalen W., Das A., Berg C. M. Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene. Nucleic Acids Res. 1987 Nov 25;15(22):9461–9469. doi: 10.1093/nar/15.22.9461. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Potter H., Dressler D. On the mechanism of genetic recombination: the maturation of recombination intermediates. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4168–4172. doi: 10.1073/pnas.74.10.4168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang M. D., Liu L., Wang B. M., Berg C. M. Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene. J Bacteriol. 1987 Sep;169(9):4228–4234. doi: 10.1128/jb.169.9.4228-4234.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whalen W. A., Berg C. M. Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C. J Bacteriol. 1982 May;150(2):739–746. doi: 10.1128/jb.150.2.739-746.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whalen W. A., Wang M. D., Berg C. M. beta-Chloro-L-alanine inhibition of the Escherichia coli alanine-valine transaminase. J Bacteriol. 1985 Dec;164(3):1350–1352. doi: 10.1128/jb.164.3.1350-1352.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]