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. 1991 Oct;173(20):6446–6452. doi: 10.1128/jb.173.20.6446-6452.1991

The absence of branched-chain amino acid and growth rate control at the internal ilvEp promoter of the ilvGMEDA operon.

E H Harms 1, H E Umbarger 1
PMCID: PMC208979  PMID: 1917871

Abstract

The question of whether the promoter ilvEp, located in the coding region of ilvM, the second structural gene in the ilvGMEDA operon, is subject to either amino acid- or growth rate-mediated regulation is examined. The experiments described here were performed with ilvEp-cat and ilvEp-lac fusions carried as single copies on the chromosome. The activity of the ilvEp promoter was found to respond neither to the availability of branched-chain amino acids nor to a wide range of growth rates between 35 to 390 min. In the absence of any known role for the products of the ilvGMEDA operon when repressing levels of branched-chain amino acids are present, there appears to be only a gratuitous role for the transcription at ilvEp.

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Selected References

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