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. 1991 Oct;173(20):6605–6611. doi: 10.1128/jb.173.20.6605-6611.1991

Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element.

M G Schmidt 1, K M Dolan 1, D B Oliver 1
PMCID: PMC208998  PMID: 1833384

Abstract

The Escherichia coli secA gene, whose translation is responsive to the proficiency of protein export within the cell, is the second gene in a three-gene operon and is flanked by gene X and mutT. By using gene fusion and oligonucleotide-directed mutagenesis techniques, we have localized this translationally regulated site to a region at the end of gene X and the beginning of secA. This region has been shown to bind SecA protein in vitro. These studies open the way for a direct investigation of the mechanism of secA regulation and its coupling to the protein secretion capability of the cell.

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Selected References

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