Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Jun;172(6):2833–2838. doi: 10.1128/jb.172.6.2833-2838.1990

Ultrastructural study of surface components of Streptococcus suis.

M Jacques 1, M Gottschalk 1, B Foiry 1, R Higgins 1
PMCID: PMC209078  PMID: 1971617

Abstract

The presence of capsular material on cells of nine reference strains of Streptococcus suis representing serotypes 1 to 8 and 1/2 was determined by transmission electron microscopy after polycationic ferritin labeling, immunostabilization, or fixation with a combination of glutaraldehyde and lysine. All the cells of the reference strains examined were covered with a layer of capsular material whose thickness varied between 20 to 30 nm and 350 to 375 nm when examined by immunostabilization. Capsular material from cells exposed to homologous antiserum was usually thicker than that from polycationic ferritin-labeled cells or cells fixed with glutaraldehyde-lysine. Negative staining revealed detectable surface structures on S. suis strains. All strains carried peritichous, thin, and flexible fimbriae with a diameter of approximately 2 nm and a length of up to 250 nm. This study indicated that morphological differences of surface structure exist among S. suis reference strains.

Full text

PDF
2833

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arends J. P., Zanen H. C. Meningitis caused by Streptococcus suis in humans. Rev Infect Dis. 1988 Jan-Feb;10(1):131–137. doi: 10.1093/clinids/10.1.131. [DOI] [PubMed] [Google Scholar]
  2. Baker C. J., Kasper D. L. Microcapsule of type III strains of group B Streptococcus: production and morphology. Infect Immun. 1976 Jan;13(1):189–194. doi: 10.1128/iai.13.1.189-194.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bayer M. E., Thurow H. Polysaccharide capsule of Escherichia coli: microscope study of its size, structure, and sites of synthesis. J Bacteriol. 1977 May;130(2):911–936. doi: 10.1128/jb.130.2.911-936.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birdsell D. C., Doyle R. J., Morgenstern M. Organization of teichoic acid in the cell wall of Bacillus subtilis. J Bacteriol. 1975 Feb;121(2):726–734. doi: 10.1128/jb.121.2.726-734.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Costerton J. W., Irvin R. T., Cheng K. J. The bacterial glycocalyx in nature and disease. Annu Rev Microbiol. 1981;35:299–324. doi: 10.1146/annurev.mi.35.100181.001503. [DOI] [PubMed] [Google Scholar]
  6. DEMOOR C. E. SEPTICAEMIC INFECTIONS IN PIGS, CAUSED BY HAEMOLYTIC STREPTOCOCCI OF NEW LANCEFIELD GROUPS DESIGNATED R, S, AND T. Antonie Van Leeuwenhoek. 1963;29:272–280. doi: 10.1007/BF02046069. [DOI] [PubMed] [Google Scholar]
  7. Elliott S. D. Streptococcal infection in young pigs. I. An immunochemical study of the causative agent (PM streptococcus). J Hyg (Lond) 1966 Jun;64(2):205–212. doi: 10.1017/s0022172400040468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Elliott S. D., Tai J. Y. The type-specific polysaccharides of Streptococcus suis. J Exp Med. 1978 Dec 1;148(6):1699–1704. doi: 10.1084/jem.148.6.1699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Erickson E. D., Doster A. R., Pokorny T. S. Isolation of Streptococcus suis from swine in Nebraska. J Am Vet Med Assoc. 1984 Sep 15;185(6):666–668. [PubMed] [Google Scholar]
  10. Handley P. S., Carter P. L., Fielding J. Streptococcus salivarius strains carry either fibrils or fimbriae on the cell surface. J Bacteriol. 1984 Jan;157(1):64–72. doi: 10.1128/jb.157.1.64-72.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Handley P. S., Carter P. L., Wyatt J. E., Hesketh L. M. Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera. Infect Immun. 1985 Jan;47(1):217–227. doi: 10.1128/iai.47.1.217-227.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Handley P. S., Jacob A. E. Some structural and physiological properties of fimbriae of Streptococcus faecalis. J Gen Microbiol. 1981 Dec;127(2):289–293. doi: 10.1099/00221287-127-2-289. [DOI] [PubMed] [Google Scholar]
  13. Jacques M., Foiry B. Electron microscopic visualization of capsular material of Pasteurella multocida types A and D labeled with polycationic ferritin. J Bacteriol. 1987 Aug;169(8):3470–3472. doi: 10.1128/jb.169.8.3470-3472.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jacques M., Foiry B., Higgins R., Mittal K. R. Electron microscopic examination of capsular material from various serotypes of Actinobacillus pleuropneumoniae. J Bacteriol. 1988 Jul;170(7):3314–3318. doi: 10.1128/jb.170.7.3314-3318.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jacques M., Graham L. Improved preservation of bacterial capsule for electron microscopy. J Electron Microsc Tech. 1989 Feb;11(2):167–169. doi: 10.1002/jemt.1060110212. [DOI] [PubMed] [Google Scholar]
  16. Kasper D. L. Bacterial capsule--old dogmas and new tricks. J Infect Dis. 1986 Mar;153(3):407–415. doi: 10.1093/infdis/153.3.407. [DOI] [PubMed] [Google Scholar]
  17. Kasper D. L., Baker C. J. Electron microscopic definition of surface antigens of group B Streptococcus. J Infect Dis. 1979 Feb;139(2):147–151. doi: 10.1093/infdis/139.2.147. [DOI] [PubMed] [Google Scholar]
  18. Kurl D. N., Haataja S., Finne J. Hemagglutination activities of group B, C, D, and G streptococci: demonstration of novel sugar-specific cell-binding activities in Streptococcus suis. Infect Immun. 1989 Feb;57(2):384–389. doi: 10.1128/iai.57.2.384-389.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lambe D. W., Jr, Mayberry-Carson K. J., Ferguson K. P. Morphological stabilization of the glycocalyces of 23 strains of five Bacteroides species using specific antisera. Can J Microbiol. 1984 Jun;30(6):809–819. doi: 10.1139/m84-124. [DOI] [PubMed] [Google Scholar]
  20. Luft J. H. Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec. 1971 Nov;171(3):347–368. doi: 10.1002/ar.1091710302. [DOI] [PubMed] [Google Scholar]
  21. Mittal K. R., Higgins R., Larivière S. Identification and serotyping of Haemophilus pleuropneumoniae by coagglutination test. J Clin Microbiol. 1983 Dec;18(6):1351–1354. doi: 10.1128/jcm.18.6.1351-1354.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Molinari A., Orefici G., Donelli G., Von Hunolstein C., Paradisi S., Arancia G. Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy. Histochem J. 1988 Sep;20(9):526–530. doi: 10.1007/BF01002651. [DOI] [PubMed] [Google Scholar]
  23. Nalbandian J., Freedman M. L., Tanzer J. M., Lovelace S. M. Ultrastructure of Mutants of Streptococcus mutans with Reference to Agglutination, Adhesion, and Extracellular Polysaccharide. Infect Immun. 1974 Nov;10(5):1170–1179. doi: 10.1128/iai.10.5.1170-1179.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ottow J. C. Ecology, physiology, and genetics of fimbriae and pili. Annu Rev Microbiol. 1975;29:79–108. doi: 10.1146/annurev.mi.29.100175.000455. [DOI] [PubMed] [Google Scholar]
  25. Perch B., Kjems E., Slot P., Pedersen K. B. Biochemical and serological properties of R, S, and RS streptococci. Acta Pathol Microbiol Scand B. 1981 Jun;89(3):167–171. doi: 10.1111/j.1699-0463.1981.tb00171_89b.x. [DOI] [PubMed] [Google Scholar]
  26. Perch B., Pedersen K. B., Henrichsen J. Serology of capsulated streptococci pathogenic for pigs: six new serotypes of Streptococcus suis. J Clin Microbiol. 1983 Jun;17(6):993–996. doi: 10.1128/jcm.17.6.993-996.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rýc M., Jelínková J., Motlová J., Wagner M. Immuno-electronmicroscopic demonstration of capsules on group-B streptococci of new serotypes and type candidates. J Med Microbiol. 1988 Feb;25(2):147–149. doi: 10.1099/00222615-25-2-147. [DOI] [PubMed] [Google Scholar]
  28. Shockman G. D., Barrett J. F. Structure, function, and assembly of cell walls of gram-positive bacteria. Annu Rev Microbiol. 1983;37:501–527. doi: 10.1146/annurev.mi.37.100183.002441. [DOI] [PubMed] [Google Scholar]
  29. Sihvonen L., Kurl D. N., Henrichsen J. Streptococcus suis isolated from pigs in Finland. Acta Vet Scand. 1988;29(1):9–13. doi: 10.1186/BF03548386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Skov Sørensen U. B., Blom J., Birch-Andersen A., Henrichsen J. Ultrastructural localization of capsules, cell wall polysaccharide, cell wall proteins, and F antigen in pneumococci. Infect Immun. 1988 Aug;56(8):1890–1896. doi: 10.1128/iai.56.8.1890-1896.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tomasz A. Surface components of Streptococcus pneumoniae. Rev Infect Dis. 1981 Mar-Apr;3(2):190–211. doi: 10.1093/clinids/3.2.190. [DOI] [PubMed] [Google Scholar]
  32. Touil F., Higgins R., Nadeau M. Isolation of Streptococcus suis from diseased pigs in Canada. Vet Microbiol. 1988 Jun;17(2):171–177. doi: 10.1016/0378-1135(88)90008-9. [DOI] [PubMed] [Google Scholar]
  33. Wagner M., Wagner B., Kubín V. R. Immunoelectron microscopic study of the location of group-specific and type-specific polysaccharide antigens on isolated walls of group B streptococci. J Gen Microbiol. 1980 Oct;120(2):369–376. doi: 10.1099/00221287-120-2-369. [DOI] [PubMed] [Google Scholar]
  34. Weerkamp A. H., Handley P. S., Baars A., Slot J. W. Negative staining and immunoelectron microscopy of adhesion-deficient mutants of Streptococcus salivarius reveal that the adhesive protein antigens are separate classes of cell surface fibril. J Bacteriol. 1986 Mar;165(3):746–755. doi: 10.1128/jb.165.3.746-755.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Windsor R. S., Elliott S. D. Streptococcal infection in young pigs. IV. An outbreak of streptococcal meningitis in weaned pigs. J Hyg (Lond) 1975 Aug;75(1):69–78. doi: 10.1017/s0022172400047070. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES