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. 1990 Jun;172(6):2955–2961. doi: 10.1128/jb.172.6.2955-2961.1990

Gene fusions with lacZ by duplication insertion in the radioresistant bacterium Deinococcus radiodurans.

E Lennon 1, K W Minton 1
PMCID: PMC209094  PMID: 2160933

Abstract

Deinococcus radiodurans is the most-studied species of a eubacterial family characterized by extreme resistance to DNA damage. We have focused on developing molecular biological techniques to investigate the genetics of this organism. We report construction of lacZ gene fusions by a method involving both in vitro splicing and the natural transformation of D. radiodurans. Numerous fusion strains were identified by expression of beta-galactosidase. Among these fusion strains, several were inducible by exposure to the DNA-damaging agent mitomycin C, and four of the inducible fusion constructs were cloned in Escherichia coli. Hybridization studies indicate that one of the damage-inducible genes contains a sequence reiterated throughout the D. radiodurans chromosome. Survival measurements show that two of the fusion strains have increased sensitivity to mitomycin C, suggesting that the fusions within these strains inactivate repair functions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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