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. 1990 Jun;172(6):3172–3179. doi: 10.1128/jb.172.6.3172-3179.1990

Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography.

B L Levinson 1, K J Kasyan 1, S S Chiu 1, T C Currier 1, J M González Jr 1
PMCID: PMC209122  PMID: 2345141

Abstract

An improved high-performance liquid chromatography separation was developed to detect and quantify beta-exotoxin production in Bacillus thuringiensis culture supernatants. Exotoxin production was assigned to a plasmid in five strains, from three subspecies (B. thuringiensis subsp. thuringiensis serotype 1, B. thuringiensis subsp. tolworthi serotype 9, and B. thuringiensis subsp. darmstadiensis serotype 10). A new exotoxin, called type II beta-exotoxin in this report, was discovered in B. thuringiensis subsp. morrisoni serotype 8ab, purified, and partially characterized. This material is more specific than type I beta-exotoxin and is very active against the Colorado potato beetle, Leptinotarsa decemlineata.

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Selected References

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  1. Benz G. On the chemical nature of the heat-stable exotoxin of Bacillus thuringiensis. Experientia. 1966 Feb 15;22(2):81–82. doi: 10.1007/BF01900160. [DOI] [PubMed] [Google Scholar]
  2. Bond R. P., Boyce C. B., French S. J. A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner. Biochem J. 1969 Sep;114(3):477–488. doi: 10.1042/bj1140477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donovan W. P., Gonzalez J. M., Jr, Gilbert M. P., Dankocsik C. Isolation and characterization of EG2158, a new strain of Bacillus thuringiensis toxic to coleopteran larvae, and nucleotide sequence of the toxin gene. Mol Gen Genet. 1988 Nov;214(3):365–372. doi: 10.1007/BF00330468. [DOI] [PubMed] [Google Scholar]
  4. González J. M., Jr, Brown B. J., Carlton B. C. Transfer of Bacillus thuringiensis plasmids coding for delta-endotoxin among strains of B. thuringiensis and B. cereus. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6951–6955. doi: 10.1073/pnas.79.22.6951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. González J. M., Jr, Dulmage H. T., Carlton B. C. Correlation between specific plasmids and delta-endotoxin production in Bacillus thuringiensis. Plasmid. 1981 May;5(3):352–365. doi: 10.1016/0147-619x(81)90010-x. [DOI] [PubMed] [Google Scholar]
  6. Haufler M., Kunz S. E. Laboratory evaluation of an exotoxin from Bacillus thuringiensis subsp. morrisoni to horn fly larvae (Diptera: Muscidae) and mice. J Econ Entomol. 1985 Jun;78(3):613–616. doi: 10.1093/jee/78.3.613. [DOI] [PubMed] [Google Scholar]
  7. Heimpel A. M. A critical review of Bacillus thuringiensis var. thuringiensis Berliner and other crystalliferous bacteria. Annu Rev Entomol. 1967;12:287–322. doi: 10.1146/annurev.en.12.010167.001443. [DOI] [PubMed] [Google Scholar]
  8. Heimpel A. M. A taxonomic key proposed for the species of the "crystalliferous bacteria". J Invertebr Pathol. 1967 Sep;9(3):364–373. doi: 10.1016/0022-2011(67)90072-9. [DOI] [PubMed] [Google Scholar]
  9. Höfte H., Whiteley H. R. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev. 1989 Jun;53(2):242–255. doi: 10.1128/mr.53.2.242-255.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson D. E. Inhibition of RNA polymerase from Bacillus thuringiensis and Escherichia coli by beta-exotoxin. Can J Microbiol. 1978 May;24(5):537–543. doi: 10.1139/m78-087. [DOI] [PubMed] [Google Scholar]
  11. Kim T. Y., Huang H. T. The beta-exotoxins of bacillus thuringiensis. I. Isolation and characterization. J Invertebr Pathol. 1970 Jan;15(1):100–108. doi: 10.1016/0022-2011(70)90103-5. [DOI] [PubMed] [Google Scholar]
  12. Klier A., Lecadet M. M., de Barjac H. Inhibition des ARN-polymérasas ADN-dépendantes de B. thuringiensis par l'exotoxine de B. thuringiensis. C R Acad Sci Hebd Seances Acad Sci D. 1974 Dec 17;277(24):2805–2808. [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  15. Matsudaira P. T., Burgess D. R. SDS microslab linear gradient polyacrylamide gel electrophoresis. Anal Biochem. 1978 Jul 1;87(2):386–396. doi: 10.1016/0003-2697(78)90688-7. [DOI] [PubMed] [Google Scholar]
  16. Sebesta K., Sternbach H. The specificity of inhibition of DNA-dependent RNA polymerase by Bacillus thuringiensis exotoxin. FEBS Lett. 1970 Jun 27;8(5):233–235. doi: 10.1016/0014-5793(70)80273-3. [DOI] [PubMed] [Google Scholar]
  17. Yamamoto T., Iizuka T. Two types of entomocidal toxins in the parasporal crystals of Bacillus thuringiensis kurstaki. Arch Biochem Biophys. 1983 Nov;227(1):233–241. doi: 10.1016/0003-9861(83)90366-1. [DOI] [PubMed] [Google Scholar]
  18. de Barjac H., Burgerjon A., Bonnefoi A. The production of heat-stable toxin by nine serotypes of Bacillus thuringiensis. J Invertebr Pathol. 1966 Dec;8(4):537–538. doi: 10.1016/0022-2011(66)90083-8. [DOI] [PubMed] [Google Scholar]
  19. de Barjac H., Cosmao Dumanoir V. Comparaison entre les exotoxines thermostables de différents sérotypes de Bacillus thuringiensis. C R Acad Sci Hebd Seances Acad Sci D. 1974 May 27;278(22):2843–2845. [PubMed] [Google Scholar]

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