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. 1990 Jun;172(6):3269–3277. doi: 10.1128/jb.172.6.3269-3277.1990

The bof gene of bacteriophage P1: DNA sequence and evidence for roles in regulation of phage c1 and ref genes.

T S Schaefer 1, J B Hays 1
PMCID: PMC209135  PMID: 2345146

Abstract

The C1 repressor of bacteriophage P1 acts via 14 or more distinct operators. This repressor represses its own synthesis as well as the synthesis of other gene products. Previously, mutation of an auxiliary regulatory gene, bof, has been shown to increase expression of some C1-regulated P1 genes (e.g., ref) but to decrease expression of others (e.g., ban). In this study the bof gene was isolated on the basis of its ability to depress stimulation of Escherichia coli chromosomal recombination by the P1 ref gene, if and only if a source of C1 was present. C1 alone, but not Bof alone, was partially effective. The bofDNA sequence encodes an 82-codon reading frame that begins with a TTG codon and includes the sites of the bof-1(Am) mutation and a bof::Tn5 null mutation. Expression of ref::lacZ and cl::lacZ fusion genes was partially repressed in trans by a P1 bof-1 prophage or by plasmid-encoded C1 alone, which was in agreement with effects on Ref-stimulated recombination and with previous indirect evidence for c1 autoregulation. Repression of both fusion genes by plasmid-encoded C1 plus Bof or by a P1 bof+ prophage was more complete. When the C1 source also included a 0.7-kilobase region upstream from C1 which encodes the coi gene, repression of both c1::lacZ and ref::lacZ by C1 alone or by C1 plus Bof was much less effective, as if Coi interfered with C1 repressor function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumstark B. R., Stovall S. R., Ashkar S. Interaction of the P1c1 repressor with P1 DNA: localization of repressor binding sites near the c1 gene. Virology. 1987 Feb;156(2):404–413. doi: 10.1016/0042-6822(87)90420-x. [DOI] [PubMed] [Google Scholar]
  3. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chesney R. H., Scott J. R. Superinfection immunity and prophage repression in phage P1. II. Mapping of the immunity-difference and ampicillin-resistance loci of P1 and phi amp. Virology. 1975 Oct;67(2):375–384. doi: 10.1016/0042-6822(75)90439-0. [DOI] [PubMed] [Google Scholar]
  5. Citron M., Velleman M., Schuster H. Three additional operators, Op21, Op68, and Op88, of bacteriophage P1. Evidence for control of the P1 dam methylase by Op68. J Biol Chem. 1989 Feb 25;264(6):3611–3617. [PubMed] [Google Scholar]
  6. Dreiseikelmann B., Velleman M., Schuster H. The c1 repressor of bacteriophage P1. Isolation and characterization of the repressor protein. J Biol Chem. 1988 Jan 25;263(3):1391–1397. [PubMed] [Google Scholar]
  7. Eliason J. L., Sternberg N. Characterization of the binding sites of c1 repressor of bacteriophage P1. Evidence for multiple asymmetric sites. J Mol Biol. 1987 Nov 20;198(2):281–293. doi: 10.1016/0022-2836(87)90313-5. [DOI] [PubMed] [Google Scholar]
  8. Fürste J. P., Pansegrau W., Frank R., Blöcker H., Scholz P., Bagdasarian M., Lanka E. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene. 1986;48(1):119–131. doi: 10.1016/0378-1119(86)90358-6. [DOI] [PubMed] [Google Scholar]
  9. Gren E. J. Recognition of messenger RNA during translational initiation in Escherichia coli. Biochimie. 1984 Jan;66(1):1–29. doi: 10.1016/0300-9084(84)90188-3. [DOI] [PubMed] [Google Scholar]
  10. Heilmann H., Reeve J. N., Pühler A. Identification of the repressor and repressor bypass (antirepressor) polypeptides of bacteriophage P1 synthesized in infected minicells. Mol Gen Genet. 1980 Apr;178(1):149–154. doi: 10.1007/BF00267223. [DOI] [PubMed] [Google Scholar]
  11. Heinzel T., Velleman M., Schuster H. ban operon of bacteriophage P1. Mutational analysis of the c1 repressor-controlled operator. J Mol Biol. 1989 Jan 5;205(1):127–135. doi: 10.1016/0022-2836(89)90370-7. [DOI] [PubMed] [Google Scholar]
  12. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  13. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  14. Johnson B. F. Suppression of the lexC (ssbA) mutation of Escherichia coli by a mutant of bacteriophage P1. Mol Gen Genet. 1982;186(1):122–126. doi: 10.1007/BF00422923. [DOI] [PubMed] [Google Scholar]
  15. Konrad E. B. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications. J Bacteriol. 1977 Apr;130(1):167–172. doi: 10.1128/jb.130.1.167-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laufer C. S., Hays J. B., Windle B. E., Schaefer T. S., Lee E. H., Hays S. L., McClure M. R. Enhancement of Escherichia coli plasmid and chromosomal recombination by the Ref function of bacteriophage P1. Genetics. 1989 Nov;123(3):465–476. doi: 10.1093/genetics/123.3.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lu S. D., Lu D., Gottesman M. Stimulation of IS1 excision by bacteriophage P1 ref function. J Bacteriol. 1989 Jun;171(6):3427–3432. doi: 10.1128/jb.171.6.3427-3432.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lurz R., Heisig A., Velleman M., Dobrinski B., Schuster H. The ban operon of bacteriophage P1. Localization of the promoter controlled by P1 repressor. J Biol Chem. 1987 Dec 5;262(34):16575–16579. [PubMed] [Google Scholar]
  19. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  20. Osborne F. A., Stovall S. R., Baumstark B. R. The c1 genes of P1 and P7. Nucleic Acids Res. 1989 Oct 11;17(19):7671–7680. doi: 10.1093/nar/17.19.7671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Prentki P., Karch F., Iida S., Meyer J. The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication. Gene. 1981 Sep;14(4):289–299. doi: 10.1016/0378-1119(81)90161-x. [DOI] [PubMed] [Google Scholar]
  22. Ptashne M., Jeffrey A., Johnson A. D., Maurer R., Meyer B. J., Pabo C. O., Roberts T. M., Sauer R. T. How the lambda repressor and cro work. Cell. 1980 Jan;19(1):1–11. doi: 10.1016/0092-8674(80)90383-9. [DOI] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Scott J. R. Immunity and repression in bacteriophages P1 and P7. Curr Top Microbiol Immunol. 1980;90:49–65. doi: 10.1007/978-3-642-67717-5_3. [DOI] [PubMed] [Google Scholar]
  25. Scott J. R., Kropf M., Mendelson L. Clear plaque mutants of phage P7. Virology. 1977 Jan;76(1):39–46. doi: 10.1016/0042-6822(77)90279-3. [DOI] [PubMed] [Google Scholar]
  26. Scott J. R., West B. W., Laping J. L. Superinfection immunity and prophage repression in phage P1. IV. The c1 repressor bypass function and the role of c4 repressor in immunity. Virology. 1978 Apr;85(2):587–600. doi: 10.1016/0042-6822(78)90463-4. [DOI] [PubMed] [Google Scholar]
  27. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  28. Simons R. W., Houman F., Kleckner N. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene. 1987;53(1):85–96. doi: 10.1016/0378-1119(87)90095-3. [DOI] [PubMed] [Google Scholar]
  29. Smith T. A., Hays J. B. Repair and recombination of nonreplicating UV-irradiated phage DNA in E. coli II. Stimulation of RecF-dependent recombination by excision repair of cyclobutane pyrimidine dimers and of other photoproducts. Mol Gen Genet. 1985;201(3):393–401. doi: 10.1007/BF00331329. [DOI] [PubMed] [Google Scholar]
  30. Sternberg N., Austin S., Hamilton D., Yarmolinsky M. Analysis of bacteriophage P1 immunity by using lambda-P1 recombinants constructed in vitro. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5594–5598. doi: 10.1073/pnas.75.11.5594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sternberg N., Austin S., Yarmolinsky M. Regulatory circuits in bacteriophage P1 as analyzed by physical dissection and reconstruction. Contrib Microbiol Immunol. 1979;6:89–99. [PubMed] [Google Scholar]
  32. Touati-Schwartz D. A new pleiotropic bacteriophage P1 mutation, bof, affecting c1 repression activity, the expression of plasmid incompatibility and the expression of certain constitutive prophage genes. Mol Gen Genet. 1979 Jul 13;174(2):189–202. doi: 10.1007/BF00268355. [DOI] [PubMed] [Google Scholar]
  33. Wandersman C., Yarmolinsky M. Bipartite control of immunity conferred by the related heteroimmune plasmid prophages, P1 and P7 (formerly phi Amp). Virology. 1977 Mar;77(1):386–400. doi: 10.1016/0042-6822(77)90435-4. [DOI] [PubMed] [Google Scholar]
  34. Windle B. E., Hays J. B. A phage P1 function that stimulates homologous recombination of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3885–3889. doi: 10.1073/pnas.83.11.3885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Windle B. E., Laufer C. S., Hays J. B. Sequence and deletion analysis of the recombination enhancement gene (ref) of bacteriophage P1: evidence for promoter-operator and attenuator-antiterminator control. J Bacteriol. 1988 Oct;170(10):4881–4889. doi: 10.1128/jb.170.10.4881-4889.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Windle B. E. Phage lambda and plasmid expression vectors with multiple cloning sites and lacZ alpha-complementation. Gene. 1986;45(1):95–99. doi: 10.1016/0378-1119(86)90136-8. [DOI] [PubMed] [Google Scholar]
  37. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  38. Zhang H., Scholl R., Browse J., Somerville C. Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res. 1988 Feb 11;16(3):1220–1220. doi: 10.1093/nar/16.3.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]

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