Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Nov;173(22):7084–7091. doi: 10.1128/jb.173.22.7084-7091.1991

Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis.

A Raymond-Denise 1, N Guillen 1
PMCID: PMC209213  PMID: 1657879

Abstract

A Bacillus subtilis strain deficient in homologous recombination was isolated from a library of Tn917lac insertion mutants. The interrupted locus consists of an open reading frame encoding a 22,823-dalton polypeptide. Analysis of the deduced amino acid sequence revealed 34% identity and 47.3% similarity with the LexA protein from Escherichia coli. The gene was designated dinR. It is located between the recA and thyA genetic markers, at 162 degrees on the B. subtilis chromosome. The dinR gene was shown to be expressed during the entire B. subtilis cellular cycle with at least a threefold increase when cells develop competence. In addition, the use of a merodiploid strain, in which a copy of the wild-type dinR gene coexists with a dinR-lacZ transcriptional fusion, demonstrated that dinR is an SOS gene and that the SOS-induced expression of dinR occurred only when a wild-type copy of dinR was present. In addition, DinR seems to regulate the expression of dinC, another SOS gene.

Full text

PDF
7084

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albano M., Hahn J., Dubnau D. Expression of competence genes in Bacillus subtilis. J Bacteriol. 1987 Jul;169(7):3110–3117. doi: 10.1128/jb.169.7.3110-3117.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheo D. L., Bayles K. W., Yasbin R. E. Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis. J Bacteriol. 1991 Mar;173(5):1696–1703. doi: 10.1128/jb.173.5.1696-1703.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ftouhi N., Guillén N. Genetic analysis of fusion recombinants in Bacillus subtilis: function of the recE gene. Genetics. 1990 Nov;126(3):487–496. doi: 10.1093/genetics/126.3.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guillen N., Weinrauch Y., Dubnau D. A. Cloning and characterization of the regulatory Bacillus subtilis competence genes comA and comB. J Bacteriol. 1989 Oct;171(10):5354–5361. doi: 10.1128/jb.171.10.5354-5361.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Horii T., Ogawa T., Ogawa H. Nucleotide sequence of the lexA gene of E. coli. Cell. 1981 Mar;23(3):689–697. doi: 10.1016/0092-8674(81)90432-3. [DOI] [PubMed] [Google Scholar]
  6. Hurstel S., Granger-Schnarr M., Daune M., Schnarr M. In vitro binding of LexA repressor to DNA: evidence for the involvement of the amino-terminal domain. EMBO J. 1986 Apr;5(4):793–798. doi: 10.1002/j.1460-2075.1986.tb04283.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lamerichs R. M., Padilla A., Boelens R., Kaptein R., Ottleben G., Rüterjans H., Granger-Schnarr M., Oertel P., Schnarr M. The amino-terminal domain of LexA repressor is alpha-helical but differs from canonical helix-turn-helix proteins: a two-dimensional 1H NMR study. Proc Natl Acad Sci U S A. 1989 Sep;86(18):6863–6867. doi: 10.1073/pnas.86.18.6863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  9. Little J. W., Hill S. A. Deletions within a hinge region of a specific DNA-binding protein. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2301–2305. doi: 10.1073/pnas.82.8.2301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Love P. E., Lyle M. J., Yasbin R. E. DNA-damage-inducible (din) loci are transcriptionally activated in competent Bacillus subtilis. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6201–6205. doi: 10.1073/pnas.82.18.6201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Love P. E., Yasbin R. E. Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5204–5208. doi: 10.1073/pnas.83.14.5204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lovett C. M., Jr, Love P. E., Yasbin R. E. Competence-specific induction of the Bacillus subtilis RecA protein analog: evidence for dual regulation of a recombination protein. J Bacteriol. 1989 May;171(5):2318–2322. doi: 10.1128/jb.171.5.2318-2322.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lovett C. M., Jr, Love P. E., Yasbin R. E., Roberts J. W. SOS-like induction in Bacillus subtilis: induction of the RecA protein analog and a damage-inducible operon by DNA damage in Rec+ and DNA repair-deficient strains. J Bacteriol. 1988 Apr;170(4):1467–1474. doi: 10.1128/jb.170.4.1467-1474.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lovett C. M., Jr, Roberts J. W. Purification of a RecA protein analogue from Bacillus subtilis. J Biol Chem. 1985 Mar 25;260(6):3305–3313. [PubMed] [Google Scholar]
  15. Moran C. P., Jr, Lang N., LeGrice S. F., Lee G., Stephens M., Sonenshein A. L., Pero J., Losick R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982;186(3):339–346. doi: 10.1007/BF00729452. [DOI] [PubMed] [Google Scholar]
  16. Ochman H., Gerber A. S., Hartl D. L. Genetic applications of an inverse polymerase chain reaction. Genetics. 1988 Nov;120(3):621–623. doi: 10.1093/genetics/120.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perry K. L., Elledge S. J., Mitchell B. B., Marsh L., Walker G. C. umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4331–4335. doi: 10.1073/pnas.82.13.4331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rudner R. Mutagenesis during transformation of Bacillus subtilis. I. An increase in "selfing' resulting from hybrid donor DNAs. Mutat Res. 1981 Oct;83(3):321–337. doi: 10.1016/0027-5107(81)90015-4. [DOI] [PubMed] [Google Scholar]
  19. Schnarr M., Granger-Schnarr M., Hurstel S., Pouyet J. The carboxy-terminal domain of the LexA repressor oligomerises essentially as the entire protein. FEBS Lett. 1988 Jul 4;234(1):56–60. doi: 10.1016/0014-5793(88)81302-4. [DOI] [PubMed] [Google Scholar]
  20. Stranathan M. C., Bayles K. W., Yasbin R. E. The nucleotide sequence of the recE+ gene of Bacillus subtilis. Nucleic Acids Res. 1990 Jul 25;18(14):4249–4249. doi: 10.1093/nar/18.14.4249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]
  22. Walker G. C. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984 Mar;48(1):60–93. doi: 10.1128/mr.48.1.60-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yasbin R. E. DNA repair in Bacillus subtilis. II. Activation of the inducible system in competent bacteria. Mol Gen Genet. 1977 Jun 8;153(2):219–225. [PubMed] [Google Scholar]
  24. Youngman P., Perkins J. B., Losick R. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions. Mol Gen Genet. 1984;195(3):424–433. doi: 10.1007/BF00341443. [DOI] [PubMed] [Google Scholar]
  25. de Vos W. M., de Vries S. C., Venema G. Cloning and expression of the Escherichia coli recA gene in Bacillus subtilis. Gene. 1983 Nov;25(2-3):301–308. doi: 10.1016/0378-1119(83)90234-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES