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. 1989 Jan;171(1):74–79. doi: 10.1128/jb.171.1.74-79.1989

Termination of DNA replication is required for cell division in Escherichia coli.

N Grossman 1, E Rosner 1, E Z Ron 1
PMCID: PMC209556  PMID: 2644214

Abstract

The correlation between termination of DNA replication and cell division in Escherichia coli was studied under conditions in which DNA replication was slowed down without inducing SOS functions. The experimental system used involved amino acid starvation of synchronized cells in the presence of methionine. The results further support the essential correlation between termination of DNA replication and initiation of division processes.

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Selected References

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  1. Burton P., Holland I. B. Two pathways of division inhibition in UV-irradiated E. coli. Mol Gen Genet. 1983;190(2):309–314. doi: 10.1007/BF00330656. [DOI] [PubMed] [Google Scholar]
  2. Béjar S., Bouché J. P. A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli. Mol Gen Genet. 1985;201(2):146–150. doi: 10.1007/BF00425651. [DOI] [PubMed] [Google Scholar]
  3. Béjar S., Bouché J. P. Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication. J Bacteriol. 1983 Feb;153(2):604–609. doi: 10.1128/jb.153.2.604-609.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dix D. E., Helmstetter C. E. Coupling between chromosome completion and cell division in Escherichia coli. J Bacteriol. 1973 Sep;115(3):786–795. doi: 10.1128/jb.115.3.786-795.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dwek R. D., Kobrin L. H., Grossman N., Ron E. Z. Synchronization of cell division in microorganisms by percoll gradients. J Bacteriol. 1980 Oct;144(1):17–21. doi: 10.1128/jb.144.1.17-21.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dwek R., Or-Gad S., Rozenhak S., Ron E. Z. Two new cell division mutants in Escherichia coli map near the terminus of chromosome replication. Mol Gen Genet. 1984;193(2):379–381. doi: 10.1007/BF00330697. [DOI] [PubMed] [Google Scholar]
  8. Grossman N., Ron E. Z. Initiation of deoxyribonucleic acid replication in Escherichia coli B: uncoupling from mass/deoxyribonucleic acid ratio. J Bacteriol. 1980 Jul;143(1):100–104. doi: 10.1128/jb.143.1.100-104.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Helmstetter C. E. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. doi: 10.1016/0022-2836(68)90424-5. [DOI] [PubMed] [Google Scholar]
  10. Hill T. M., Henson J. M., Kuempel P. L. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754–1758. doi: 10.1073/pnas.84.7.1754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huisman O., D'Ari R., George J. Inducible sfi dependent division inhibition in Escherichia coli. Mol Gen Genet. 1980;177(4):629–636. doi: 10.1007/BF00272673. [DOI] [PubMed] [Google Scholar]
  12. Inouye M., Pardee A. B. Requirement of polyamines for bacterial division. J Bacteriol. 1970 Mar;101(3):770–776. doi: 10.1128/jb.101.3.770-776.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jaffé A., D'Ari R., Norris V. SOS-independent coupling between DNA replication and cell division in Escherichia coli. J Bacteriol. 1986 Jan;165(1):66–71. doi: 10.1128/jb.165.1.66-71.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jones N. C., Donachie W. D. Chromosome replication, transcription and control of cell division in Escherichia coli. Nat New Biol. 1973 May 23;243(125):100–103. [PubMed] [Google Scholar]
  15. Koppes L. H., Woldringh C. L., Nanninga N. Size variations and correlation of different cell cycle events in slow-growing Escherichia coli. J Bacteriol. 1978 May;134(2):423–433. doi: 10.1128/jb.134.2.423-433.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Loehr J., Hanawalt P. On the termination of the DNA replication cycle in Escherichia coli. J Mol Biol. 1977 Nov 25;117(1):85–94. doi: 10.1016/0022-2836(77)90024-9. [DOI] [PubMed] [Google Scholar]
  17. Lombardini J. B., Talalay P. Formation, functions and regulatory importance of S-adenosyl-L-methionine. Adv Enzyme Regul. 1970;9:349–384. doi: 10.1016/s0065-2571(71)80054-7. [DOI] [PubMed] [Google Scholar]
  18. Marunouchi T., Messer W. Replication of a specific terminal chromosome segment in Escherichia coli which is required for cell division. J Mol Biol. 1973 Jun 25;78(1):211–228. doi: 10.1016/0022-2836(73)90439-7. [DOI] [PubMed] [Google Scholar]
  19. Messer W., Bellekes U., Lother H. Effect of dam methylation on the activity of the E. coli replication origin, oriC. EMBO J. 1985 May;4(5):1327–1332. doi: 10.1002/j.1460-2075.1985.tb03780.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Parnes J. R., Velan B., Felsenfeld A., Ramanathan L., Ferrini U., Appella E., Seidman J. G. Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253–2257. doi: 10.1073/pnas.78.4.2253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pato M. L. Alterations of the rate of movement of deoxyribonucleic acid replication forks. J Bacteriol. 1975 Jul;123(1):272–277. doi: 10.1128/jb.123.1.272-277.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pierucci O., Helmstetter C. E. Chromosome replication, protein synthesis and cell division in Escherichia coli. Fed Proc. 1969 Nov-Dec;28(6):1755–1760. [PubMed] [Google Scholar]
  23. Projan S. J., Carleton S., Novick R. P. Determination of plasmid copy number by fluorescence densitometry. Plasmid. 1983 Mar;9(2):182–190. doi: 10.1016/0147-619x(83)90019-7. [DOI] [PubMed] [Google Scholar]
  24. Ron E., Grossman N., Helmstetter C. E. Control of cell division in Escherichia coli: effect of amino acid starvation. J Bacteriol. 1977 Feb;129(2):569–573. doi: 10.1128/jb.129.2.569-573.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tormo A., Martínez-Salas E., Vicente M. Involvement of the ftsA gene product in late stages of the Escherichia coli cell cycle. J Bacteriol. 1980 Feb;141(2):806–813. doi: 10.1128/jb.141.2.806-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Trueba F. J., Woldringh C. L. Changes in cell diameter during the division cycle of Escherichia coli. J Bacteriol. 1980 Jun;142(3):869–878. doi: 10.1128/jb.142.3.869-878.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. UMBARGER H. E., BROWN B. Isoleucine and valine metabolism in Escherichia coli. V. Antagonism between isoleucine and valine. J Bacteriol. 1955 Aug;70(2):241–248. doi: 10.1128/jb.70.2.241-248.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Woldringh C. L., de Jong M. A., van den Berg W., Koppes L. Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth. J Bacteriol. 1977 Jul;131(1):270–279. doi: 10.1128/jb.131.1.270-279.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]
  30. de Massy B., Béjar S., Louarn J., Louarn J. M., Bouché J. P. Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759–1763. doi: 10.1073/pnas.84.7.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]

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