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. 1989 Jan;171(1):80–82. doi: 10.1128/jb.171.1.80-82.1989

Apparent minimal size required for cell division in Escherichia coli.

N Grossman 1, E Z Ron 1
PMCID: PMC209557  PMID: 2644216

Abstract

The experiments described in this report were designed to find out whether there is a minimal size threshold for cell division or for DNA replication in Escherichia coli. Cells with decreasing size (or mass) were obtained by successive amino acid starvations. Following two starvations, the cells were at least 30% smaller than unstarved newborn cells. The results suggest that this size is below a minimal size threshold for cell division but not for initiation of DNA replication.

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Selected References

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  1. Béjar S., Bouché J. P. Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication. J Bacteriol. 1983 Feb;153(2):604–609. doi: 10.1128/jb.153.2.604-609.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
  4. Dwek R. D., Kobrin L. H., Grossman N., Ron E. Z. Synchronization of cell division in microorganisms by percoll gradients. J Bacteriol. 1980 Oct;144(1):17–21. doi: 10.1128/jb.144.1.17-21.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grossman N., Ron E. Z. Initiation of deoxyribonucleic acid replication in Escherichia coli B: uncoupling from mass/deoxyribonucleic acid ratio. J Bacteriol. 1980 Jul;143(1):100–104. doi: 10.1128/jb.143.1.100-104.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helmstetter C. E. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. doi: 10.1016/0022-2836(68)90424-5. [DOI] [PubMed] [Google Scholar]
  7. Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
  8. Messer W., Bellekes U., Lother H. Effect of dam methylation on the activity of the E. coli replication origin, oriC. EMBO J. 1985 May;4(5):1327–1332. doi: 10.1002/j.1460-2075.1985.tb03780.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parnes J. R., Velan B., Felsenfeld A., Ramanathan L., Ferrini U., Appella E., Seidman J. G. Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253–2257. doi: 10.1073/pnas.78.4.2253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Projan S. J., Carleton S., Novick R. P. Determination of plasmid copy number by fluorescence densitometry. Plasmid. 1983 Mar;9(2):182–190. doi: 10.1016/0147-619x(83)90019-7. [DOI] [PubMed] [Google Scholar]
  11. Ron E. Z., Rozenhak S., Grossman N. Synchronization of cell division in Escherichia coli by amino acid starvation: strain specificity. J Bacteriol. 1975 Jul;123(1):374–376. doi: 10.1128/jb.123.1.374-376.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ron E., Grossman N., Helmstetter C. E. Control of cell division in Escherichia coli: effect of amino acid starvation. J Bacteriol. 1977 Feb;129(2):569–573. doi: 10.1128/jb.129.2.569-573.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. UMBARGER H. E., BROWN B. Isoleucine and valine metabolism in Escherichia coli. V. Antagonism between isoleucine and valine. J Bacteriol. 1955 Aug;70(2):241–248. doi: 10.1128/jb.70.2.241-248.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Woldringh C. L., de Jong M. A., van den Berg W., Koppes L. Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth. J Bacteriol. 1977 Jul;131(1):270–279. doi: 10.1128/jb.131.1.270-279.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]

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