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. 1989 Jan;171(1):349–352. doi: 10.1128/jb.171.1.349-352.1989

Chromosome and cell wall segregation in Streptococcus faecium ATCC 9790.

M L Higgins 1, D Glaser 1, D T Dicker 1, E T Zito 1
PMCID: PMC209594  PMID: 2914850

Abstract

Segregation was studied by measuring the positions of autoradiographic grain clusters in chains formed from single cells containing on average less than one radiolabeled chromosome strand. The degree to which chromosomal and cell wall material cosegregated was quantified by using the methods of S. Cooper and M. Weinberger, dividing the number of chains labeled at the middle. This analysis indicated that in contrast to chromosomal segregation in Escherichia coli and, in some studies, to that in gram-positive rods, chromosomal segregation in Streptococcus faecium was slightly nonrandom and did not vary with growth rate. Results were not significantly affected by strand exchange. In contrast, labeled cell wall segregated predominantly nonrandomly.

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Selected References

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  1. Boothby D., Daneo-Moore L., Higgins M. L., Coyette J., Shockman G. D. Turnover of bacterial cell wall peptidoglycans. J Biol Chem. 1973 Mar 25;248(6):2161–2169. [PubMed] [Google Scholar]
  2. Chai N. C., Lark K. G. Segregation of deoxyribonucleic acid in bacteria: association of the segregating unit with the cell envelope. J Bacteriol. 1967 Aug;94(2):415–421. doi: 10.1128/jb.94.2.415-421.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper S., Schwimmer M., Scanlon S. Probabilistic behavior of DNA segregation in Escherichia coli. J Bacteriol. 1978 Apr;134(1):60–65. doi: 10.1128/jb.134.1.60-65.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper S., Weinberger M. Medium-dependent variation of deoxyribonucleic acid segregation in Escherichia coli. J Bacteriol. 1977 Apr;130(1):118–127. doi: 10.1128/jb.130.1.118-127.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cánovas J. L., Tresguerres E. F., Yousif A. M., López-Sáez J. F., Navarrete M. H. DNA segregation in Escherichia coli cells with 5-bromodeoxyuridine-substituted nucleoids. J Bacteriol. 1984 Apr;158(1):128–133. doi: 10.1128/jb.158.1.128-133.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibson C. W., Daneo-Moore L., Higgins M. L. Analysis of initiation of sites of cell wall growth in Streptococcus faecium during a nutritional shift. J Bacteriol. 1984 Dec;160(3):935–942. doi: 10.1128/jb.160.3.935-942.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Helmstetter C. E., Leonard A. C. Mechanism for chromosome and minichromosome segregation in Escherichia coli. J Mol Biol. 1987 Sep 20;197(2):195–204. doi: 10.1016/0022-2836(87)90118-5. [DOI] [PubMed] [Google Scholar]
  8. Higgins M. L., Shockman G. D. Study of cycle of cell wall assembly in Streptococcus faecalis by three-dimensional reconstructions of thin sections of cells. J Bacteriol. 1976 Sep;127(3):1346–1358. doi: 10.1128/jb.127.3.1346-1358.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hitchins A. D. Polarity and topology of DNA segregation and septation in cells and sporangia of the bacilli. Can J Microbiol. 1978 Oct;24(10):1104–1134. [PubMed] [Google Scholar]
  10. Lark K. G., Bird R. E. Segregation of the conserved units of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1965 Nov;54(5):1444–1450. doi: 10.1073/pnas.54.5.1444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lederberg J. Linear Inheritance in Transductional Clones. Genetics. 1956 Nov;41(6):845–871. doi: 10.1093/genetics/41.6.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lin E. C., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli. J Bacteriol. 1971 Oct;108(1):375–385. doi: 10.1128/jb.108.1.375-385.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Parks L. C., Rigney D., Daneo-Moore L., Higgins M. L. Membrane-DNA attachment sites in Streptococcus faecalis cells grown at different rates. J Bacteriol. 1982 Oct;152(1):191–200. doi: 10.1128/jb.152.1.191-200.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pierucci O., Zuchowski C. Non-random segregation of DNA strands in Escherichia coli B-r. J Mol Biol. 1973 Nov 5;80(3):477–503. doi: 10.1016/0022-2836(73)90417-8. [DOI] [PubMed] [Google Scholar]
  16. Ryter A. Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriol Rev. 1968 Mar;32(1):39–54. doi: 10.1128/br.32.1.39-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ryter A., Hirota Y., Jacob F. DNA-membrane complex and nuclear segregation in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:669–676. doi: 10.1101/sqb.1968.033.01.076. [DOI] [PubMed] [Google Scholar]
  18. Schlaeppi J. M., Karamata D. Cosegregation of cell wall and DNA in Bacillus subtilis. J Bacteriol. 1982 Dec;152(3):1231–1240. doi: 10.1128/jb.152.3.1231-1240.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schlaeppi J. M., Schaefer O., Karamata D. Cell wall and DNA cosegregation in Bacillus subtilis studied by electron microscope autoradiography. J Bacteriol. 1985 Oct;164(1):130–135. doi: 10.1128/jb.164.1.130-135.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sonnenfeld E. M., Koch A. L., Doyle R. J. Cellular location of origin and terminus of replication in Bacillus subtilis. J Bacteriol. 1985 Sep;163(3):895–899. doi: 10.1128/jb.163.3.895-899.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TOENNIES G., ISZARD L., ROGERS N. B., SHOCKMAN G. D. Cell multiplication studied with an electronic particle counter. J Bacteriol. 1961 Dec;82:857–866. doi: 10.1128/jb.82.6.857-866.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

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